##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccI AclI AcyI AgeI
AhdI Aor51HI ApaI AscI AspEI
AspI AviII AvrII BbeI BcgI
BcnI BlnI BlpI Bpu1102I BsaHI
BsiEI BsiWI BsmBI BspDI BsrFI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I FauI
FseI FspI HaeII HhaI Hin1I
HinP1I KasI KspI MluI NaeI
NarI NciI NcoI NgoMIV NheI
NotI PflFI PinAI PmeI PshAI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
Acc65I 89 GGTACC G^GTACC
AccII 48 CGCG CG^CG
AccIII 71 TCCGGA T^CCGGA
AflII 235 CTTAAG C^TTAAG
Alw44I 4297 GTGCAC G^TGCAC
AlwNI 3958 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 905 CCTTAGG CC^T[ACGT]AGG
ApaLI 4297 GTGCAC G^TGCAC
Asp700I 1199 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 89 GGTACC G^GTACC
AvaI 1644 CTCGAG C^[CT]CG[GA]G
BalI 658 TGGCCA TGG^CCA
BanI 89 GGTACC G^G[CT][GA]CC
BanII 3710 GAGCTC G[GA]GC[CT]^C
BbrPI 1603 CACGTG CAC^GTG
BbvCI 2212 GCTGAGG CCTCAGC(-5/-2)
BfrI 235 CTTAAG C^TTAAG
BpmI 2396 CTCCAG CTGGAG(16/14)
BsaI 526 GAGACC GGTCTC(1/5)
BsaWI 71 TCCGGA [AT]^CCGG[AT]
BseAI 71 TCCGGA T^CCGGA
BsmI 3348 GCATTC GAATGC(1/-1)
BsoBI 1644 CTCGAG C^[CT]CG[GA]G
BspEI 71 TCCGGA T^CCGGA
BspLU11I 3553 ACATGT A^CATGT
BsrBI 14 GAGCGG CCGCTC(-3/-3)
BstAPI 1435 GCATTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3354 TTCGAA TT^CGAA
BstUI 48 CGCG CG^CG
BstXI 1656 CCACCATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 905 CCTTAGG CC^T[ACGT]AGG
Cac8I 2361 GCAAGC GC[ACGT]^[ACGT]GC
DrdI 2638 GACAGTAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 658 TGGCCA [CT]^GGCC[GA]
EarI 2695 CTCTTC CTCTTC(1/4)
Eco81I 905 CCTTAGG CC^T[ACGT]AGG
HapII 72 CCGG C^CGG
HgaI 51 GACGC GACGC(5/10)
HindIII 3780 AAGCTT A^AGCTT
HpaI 3855 GTTAAC GTT^AAC
HpaII 72 CCGG C^CGG
KpnI 89 GGTACC GGTAC^C
Ksp632I 2695 CTCTTC CTCTTC(1/4)
MluNI 658 TGGCCA TGG^CCA
MroI 71 TCCGGA T^CCGGA
MscI 658 TGGCCA TGG^CCA
MspI 72 CCGG C^CGG
MvnI 48 CGCG CG^CG
NdeI 2687 CATATG CA^TATG
NruI 47 TCGCGA TCG^CGA
NspV 3354 TTCGAA TT^CGAA
PacI 2506 TTAATTAA TTAAT^TAA
PaeR7I 1644 CTCGAG C^TCGAG
PleI 3954 GAGTC GAGTC(4/5)
PmaCI 1603 CACGTG CAC^GTG
PmlI 1603 CACGTG CAC^GTG
SacI 3710 GAGCTC GAGCT^C
SexAI 85 ACCTGGT A^CC[AT]GGT
SfiI 772 GGCCTCTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 3354 TTCGAA TT^CGAA
XbaI 3515 TCTAGA T^CTAGA
XhoI 1644 CTCGAG C^TCGAG
XmnI 1199 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AflIII 802 ACGTGT A^C[GA][CT]GT
3553 ACATGT
BclI 947 TGATCA T^GATCA
4381 TGATCA
BglI 773 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1841 GCCCAAATGGC
BglII 1422 AGATCT A^GATCT
2149 AGATCT
BsaAI 801 TACGTG [CT]AC^GT[GA]
1603 CACGTG
BsaBI 871 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2167 GATTGGGATC
BseRI 1967 GAGGAG GAGGAG(10/8)
2576 GAGGAG
BsgI 4522 CTGCAC GTGCAG(16/14)
3004 GTGCAG
Bsp1407I 1429 TGTACA T^GTACA
3965 TGTACA
BspHI 809 TCATGA T^CATGA
3415 TCATGA
BspMI 1458 ACCTGC ACCTGC(4/8)
2521 GCAGGT
BsrDI 1313 CATTGC GCAATG(2/0)
2807 GCAATG
BsrGI 1429 TGTACA T^GTACA
3965 TGTACA
DraII 666 GGGTCCT [GA]G^G[ACGT]CC[CT]
2523 AGGTCCT
DraIII 1868 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
4287 CACTCTGTG
EcoO109I 666 GGGTCCT [GA]G^G[ACGT]CC[CT]
2523 AGGTCCT
EcoRI 2500 GAATTC G^AATTC
3882 GAATTC
EcoRV 1037 GATATC GAT^ATC
2080 GATATC
EcoT22I 3622 ATGCAT ATGCA^T
4351 ATGCAT
FbaI 947 TGATCA T^GATCA
4381 TGATCA
HincII 2018 GTCAAC GT[CT]^[GA]AC
3855 GTTAAC
HindII 2018 GTCAAC GT[CT]^[GA]AC
3855 GTTAAC
MamI 871 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2167 GATTGGGATC
MfeI 479 CAATTG C^AATTG
3832 CAATTG
MunI 479 CAATTG C^AATTG
3832 CAATTG
NsiI 3622 ATGCAT ATGCA^T
4351 ATGCAT
PflMI 530 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3685 CCATTTTGTGG
PpuMI 666 GGGTCCT [GA]G^G[AT]CC[CT]
2523 AGGTCCT
PstI 1460 CTGCAG CTGCA^G
3315 CTGCAG
PvuII 303 CAGCTG CAG^CTG
2768 CAGCTG
RcaI 809 TCATGA T^CATGA
3415 TCATGA
ScaI 2400 AGTACT AGT^ACT
3008 AGTACT
SfcI 1460 CTGCAG C^T[GA][CT]AG
3315 CTGCAG
SspBI 1429 TGTACA T^GTACA
3965 TGTACA
SspI 1509 AATATT AAT^ATT
1825 AATATT
StuI 771 AGGCCT AGG^CCT
4596 AGGCCT
Tsp45I 231 GTCAC ^GT[GC]AC
982 GTCAC
Van91I 530 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3685 CCATTTTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AspHI AvaII
BamHI BbsI BbvI BciVI BfaI
BmyI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cfr13I
DdeI DpnII DraI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XcmI XhoII