##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccII AccIII AflII AgeI
AscI AspI AvrII BamHI BbrPI
BcgI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BspEI
BsrBI BsrFI BssHII BssSI BstBI
BstEII BstPI BstUI CelII Cfr10I
CpoI DrdI EagI EclXI Eco52I
EcoO65I FauI FseI KspI MluI
MroI MspA1I MvnI NaeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PvuI PvuII RsrII
SacI SacII SfiI SfuI SgrAI
SmaI SnaBI SpeI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 1700 GGTACC G^GTACC
AclI 4349 AACGTT AA^CGTT
AflIII 3822 ACATGT A^C[GA][CT]GT
AhdI 958 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2230 GTGCAC G^TGCAC
Aor51HI 1378 AGCGCT AGC^GCT
ApaI 2345 GGGCCC GGGCC^C
ApaLI 2230 GTGCAC G^TGCAC
Asp718I 1700 GGTACC G^GTACC
AspEI 958 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 427 CCCGAG C^[CT]CG[GA]G
AviII 3654 TGCGCA TGC^GCA
BalI 6428 TGGCCA TGG^CCA
BbvCI 3167 GCTGAGG CCTCAGC(-5/-2)
BclI 5343 TGATCA T^GATCA
BsaI 3050 GGTCTC GGTCTC(1/5)
BseRI 2218 CTCCTC GAGGAG(10/8)
BsoBI 427 CCCGAG C^[CT]CG[GA]G
BspDI 1754 ATCGAT AT^CGAT
BspHI 5966 TCATGA T^CATGA
BspLU11I 3822 ACATGT A^CATGT
Bst1107I 4317 GTATAC GTA^TAC
BstZ17I 4317 GTATAC GTA^TAC
ClaI 1754 ATCGAT AT^CGAT
EaeI 6428 TGGCCA [CT]^GGCC[GA]
Eam1105I 958 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1378 AGCGCT AGC^GCT
EcoNI 339 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4193 GAATTC G^AATTC
EcoRV 324 GATATC GAT^ATC
EcoT22I 1946 ATGCAT ATGCA^T
FbaI 5343 TGATCA T^GATCA
FspI 3654 TGCGCA TGC^GCA
HpaI 6434 GTTAAC GTT^AAC
KpnI 1700 GGTACC GGTAC^C
MfeI 2753 CAATTG C^AATTG
MluNI 6428 TGGCCA TGG^CCA
MscI 6428 TGGCCA TGG^CCA
MunI 2753 CAATTG C^AATTG
NcoI 4236 CCATGG C^CATGG
NsiI 1946 ATGCAT ATGCA^T
Psp1406I 4349 AACGTT AA^CGTT
RcaI 5966 TCATGA T^CATGA
SalI 2314 GTCGAC G^TCGAC
SapI 3560 GCTCTTC GCTCTTC(1/4)
SbfI 378 CCTGCAGG CCTGCA^GG
SexAI 1106 ACCTGGT A^CC[AT]GGT
SphI 4222 GCATGC GCATG^C
Sse8387I 378 CCTGCAGG CCTGCA^GG
SwaI 3944 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 1684 GGCGCC G[GA]^CG[CT]C
3626 GGCGCC
AlwNI 382 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1064 CAGTGACTG
AocI 339 CCTCAGG CC^T[ACGT]AGG
400 CCTCAGG
AseI 5112 ATTAAT AT^TAAT
5386 ATTAAT
AsnI 5112 ATTAAT AT^TAAT
5386 ATTAAT
Asp700I 2455 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4123 GAATAAGTTC
BbeI 1684 GGCGCC GGCGC^C
3626 GGCGCC
BbsI 718 GAAGAC GAAGAC(2/6)
1035 GTCTTC
BcnI 1308 CCGGG CC^[GC]GG
1689 CCCGG
BglII 939 AGATCT A^GATCT
5717 AGATCT
BpuAI 718 GAAGAC GAAGAC(2/6)
1035 GTCTTC
BsaBI 2864 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3003 GATGGGGATC
BsaHI 1684 GGCGCC G[GA]^CG[CT]C
3626 GGCGCC
Bsu36I 339 CCTCAGG CC^T[ACGT]AGG
400 CCTCAGG
DraIII 1270 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3704 CACGGGGTG
Eco81I 339 CCTCAGG CC^T[ACGT]AGG
400 CCTCAGG
HapII 1308 CCGG C^CGG
1690 CCGG
Hin1I 1684 GGCGCC G[GA]^CG[CT]C
3626 GGCGCC
HpaII 1308 CCGG C^CGG
1690 CCGG
KasI 1684 GGCGCC G^GCGCC
3626 GGCGCC
MamI 2864 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3003 GATGGGGATC
MspI 1308 CCGG C^CGG
1690 CCGG
NarI 1684 GGCGCC GG^CGCC
3626 GGCGCC
NciI 1308 CCGGG CC^[GC]GG
1689 CCCGG
NdeI 4921 CATATG CA^TATG
5091 CATATG
PflMI 1077 CCATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4544 CCAAAAGATGG
PshBI 5112 ATTAAT AT^TAAT
5386 ATTAAT
SfoI 1684 GGCGCC GGC^GCC
3626 GGCGCC
SmlI 2301 CTTGAG C^T[CT][GA]AG
3299 CTTGAG
StuI 1988 AGGCCT AGG^CCT
2120 AGGCCT
TaqI 1755 TCGA T^CGA
2315 TCGA
TthHB8I 1755 TCGA T^CGA
2315 TCGA
Van91I 1077 CCATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4544 CCAAAAGATGG
XmnI 2455 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4123 GAATAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BanI
BanII BbvI BciVI BfaI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HgaI HhaI HinP1I HincII HindII
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SspBI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XbaI
XcmI XhoII