##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AvrII       BamHI       BfrI        
BlnI        BseAI       BsiEI       BsiWI       BsmI        
BspDI       BspEI       BsrBI       BssHII      Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        DraI        
EagI        EclXI       Eco52I      EcoNI       EcoRI       
EcoRV       EcoT22I     FseI        HpaI        KpnI        
KspI        MfeI        MluI        MroI        MunI        
NdeI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PinAI       PmeI        PshBI       
Psp1406I    PvuI        RsrII       SacII       SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
SwaI        Tth111I     XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI          673  GTCGAC               GT^[AC][GT]AC
AhdI          665  GACGTGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         3072  CCTGAGG              CC^T[ACGT]AGG
ApaI         3078  GGGCCC               GGGCC^C
AspEI         665  GACGTGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        2487  TGCGCA               TGC^GCA
BbeI         1721  GGCGCC               GGCGC^C
BbvCI        2922  CCTCAGC              CCTCAGC(-5/-2)
BcgI          403  CGAGCTCTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2440  GGATAC               GTATCC(6/5)
BglI          888  GCCTCAATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        3403  AGATCT               A^GATCT
BsmBI        3256  GAGACG               CGTCTC(1/5)
Bsp1407I      297  TGTACA               T^GTACA
BspMI        1675  GCAGGT               ACCTGC(4/8)
BsrFI         707  GCCGGC               [GA]^CCGG[CT]
BsrGI         297  TGTACA               T^GTACA
BstAPI         28  GCAGCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2041  GGTCACC              G^GT[ACGT]ACC
BstPI        2041  GGTCACC              G^GT[ACGT]ACC
Bsu36I       3072  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        707  GCCGGC               [GA]^CCGG[CT]
DrdI          512  GACCACGCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      665  GACGTGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       3072  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      2041  GGTCACC              G^GT[ACGT]ACC
FspI         2487  TGCGCA               TGC^GCA
KasI         1721  GGCGCC               G^GCGCC
NaeI          707  GCCGGC               GCC^GGC
NarI         1721  GGCGCC               GG^CGCC
NgoMIV        707  GCCGGC               G^CCGGC
NheI         2740  GCTAGC               G^CTAGC
PaeR7I       2809  CTCGAG               C^TCGAG
PshAI         676  GACTGATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI          673  GTCGAC               G^TCGAC
SapI          173  GAAGAGC              GCTCTTC(1/4)
SbfI         1072  CCTGCAGG             CCTGCA^GG
SexAI        2781  ACCAGGT              A^CC[AT]GGT
SfoI         1721  GGCGCC               GGC^GCC
SmaI         1419  CCCGGG               CCC^GGG
Sse8387I     1072  CCTGCAGG             CCTGCA^GG
SspBI         297  TGTACA               T^GTACA
SspI         3342  AATATT               AAT^ATT
XhoI         2809  CTCGAG               C^TCGAG
XmaCI        1419  CCCGGG               C^CCGGG
XmaI         1419  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

AccII         725  CGCG                 CG^CG
             2425  CGCG                
AcsI         2841  GAATTT               [GA]^AATT[CT]
             3506  GAATTT              
AlwI           43  GATCC                GGATC(4/5)
              460  GATCC               
Aor51HI       651  AGCGCT               AGC^GCT
             2391  AGCGCT              
ApoI         2841  GAATTT               [GA]^AATT[CT]
             3506  GAATTT              
BbrPI         224  CACGTG               CAC^GTG
             2063  CACGTG              
BclI         2277  TGATCA               T^GATCA
             3281  TGATCA              
BfaI          812  CTAG                 C^TAG
             2741  CTAG                
BlpI         1540  GCTCAGC              GC^T[ACGT]AGC
             1918  GCTCAGC             
Bpu1102I     1540  GCTCAGC              GC^T[ACGT]AGC
             1918  GCTCAGC             
BsaBI         454  GATTAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1318  GATACTCATC          
BsaI          956  GAGACC               GGTCTC(1/5)
             3231  GGTCTC              
BsaWI        1464  ACCGGA               [AT]^CCGG[AT]
             2830  ACCGGA              
BsgI         1300  CTGCAC               GTGCAG(16/14)
             1673  GTGCAG              
BspHI        1911  TCATGA               T^CATGA
             3323  TCATGA              
BspLU11I      300  ACATGT               A^CATGT
             2049  ACATGT              
BsrDI         844  CATTGC               GCAATG(2/0)
             1572  GCAATG              
BssSI         311  CACGAG               CACGAG(-5/-1)
             1118  CACGAG              
BstUI         725  CGCG                 CG^CG
             2425  CGCG                
CelII        1540  GCTCAGC              GC^T[ACGT]AGC
             1918  GCTCAGC             
DraIII       1685  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2780  CACCAGGTG           
Eco47III      651  AGCGCT               AGC^GCT
             2391  AGCGCT              
FbaI         2277  TGATCA               T^GATCA
             3281  TGATCA              
HincII        673  GTCGAC               GT[CT]^[GA]AC
             2339  GTCAAC              
HindII        673  GTCGAC               GT[CT]^[GA]AC
             2339  GTCAAC              
HindIII      1436  AAGCTT               A^AGCTT
             3547  AAGCTT              
MaeI          812  CTAG                 C^TAG
             2741  CTAG                
MamI          454  GATTAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1318  GATACTCATC          
MvnI          725  CGCG                 CG^CG
             2425  CGCG                
NcoI          546  CCATGG               C^CATGG
              747  CCATGG              
PmaCI         224  CACGTG               CAC^GTG
             2063  CACGTG              
PmlI          224  CACGTG               CAC^GTG
             2063  CACGTG              
PpuMI        1077  AGGACCT              [GA]G^G[AT]CC[CT]
             1564  GGGACCC             
PstI          370  CTGCAG               CTGCA^G
             1073  CTGCAG              
RcaI         1911  TCATGA               T^CATGA
             3323  TCATGA              
ScaI         1554  AGTACT               AGT^ACT
             1884  AGTACT              
StuI          126  AGGCCT               AGG^CCT
             2034  AGGCCT              
TfiI         1372  GATTC                G^A[AT]TC
             1833  GATTC               
Tsp509I      2842  AATT                 ^AATT
             3507  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (  104 enzymes) #
#########################################

AciI        AcyI        AfaI        AflIII      AluI        
Alw44I      AlwNI       ApaLI       AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbsI        
BbvI        BcnI        BmyI        BpmI        BpuAI       
BsaAI       BsaHI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstXI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        Tru9I       TseI        Tsp45I      TspRI       
TthHB8I     Van91I      XcmI        XhoII