##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI BsiEI BsiWI BspDI
BssHII Bst1107I BstZ17I ClaI CpoI
EagI EclXI Eco47III Eco52I EcoRI
EcoRV FseI HpaI KspI MfeI
MunI NotI NruI PacI PaeR7I
PinAI PmeI PshAI PshBI PvuI
RsrII SacII SalI SapI SfiI
SgrAI SmaI SnaBI SpeI SphI
StuI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 75 GACGTC GACGT^C
Acc65I 5343 GGTACC G^GTACC
AclI 706 AACGTT AA^CGTT
AflII 4245 CTTAAG C^TTAAG
AhdI 2038 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2594 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 5343 GGTACC G^GTACC
AspEI 2038 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2437 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 6040 TGCGCA TGC^GCA
AvrII 5756 CCTAGG C^CTAGG
BbeI 5987 GGCGCC GGCGC^C
BbrPI 2059 CACGTG CAC^GTG
BbvCI 5774 CCTCAGC CCTCAGC(-5/-2)
BcgI 2265 CGAGGCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1604 GTATCC GTATCC(6/5)
BfrI 4245 CTTAAG C^TTAAG
BglI 5247 GCCAACGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 6067 AGATCT A^GATCT
BlnI 5756 CCTAGG C^CTAGG
BsaBI 2554 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BstBI 46 TTCGAA TT^CGAA
BstEII 5551 GGTCACC G^GT[ACGT]ACC
BstPI 5551 GGTCACC G^GT[ACGT]ACC
DraIII 5706 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2038 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 5161 CCTAGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 5551 GGTCACC G^GT[ACGT]ACC
EcoT22I 854 ATGCAT ATGCA^T
FspI 6040 TGCGCA TGC^GCA
KasI 5987 GGCGCC G^GCGCC
KpnI 5343 GGTACC GGTAC^C
MamI 2554 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 4 ACGCGT A^CGCGT
NaeI 2375 GCCGGC GCC^GGC
NarI 5987 GGCGCC GG^CGCC
NdeI 3798 CATATG CA^TATG
NgoMIV 2375 GCCGGC G^CCGGC
NheI 2683 GCTAGC G^CTAGC
NsiI 854 ATGCAT ATGCA^T
NspV 46 TTCGAA TT^CGAA
PflFI 2437 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2059 CACGTG CAC^GTG
PmlI 2059 CACGTG CAC^GTG
Psp1406I 706 AACGTT AA^CGTT
SbfI 1851 CCTGCAGG CCTGCA^GG
SfoI 5987 GGCGCC GGC^GCC
SfuI 46 TTCGAA TT^CGAA
Sse8387I 1851 CCTGCAGG CCTGCA^GG
Tth111I 2437 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3308 TCTAGA T^CTAGA
XcmI 355 CCAGTCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 2137 GTCTAC GT^[AC][GT]AC
3261 GTCTAC
AccII 5 CGCG CG^CG
5513 CGCG
ApaI 2005 GGGCCC GGGCC^C
2130 GGGCCC
BalI 296 TGGCCA TGG^CCA
5743 TGGCCA
BamHI 1660 GGATCC G^GATCC
5839 GGATCC
BlpI 148 GCTCAGC GC^T[ACGT]AGC
2640 GCTGAGC
Bpu1102I 148 GCTCAGC GC^T[ACGT]AGC
2640 GCTGAGC
BsaAI 2059 CACGTG [CT]AC^GT[GA]
2287 TACGTG
BsmBI 77 CGTCTC CGTCTC(1/5)
3422 CGTCTC
BsmI 1014 GAATGC GAATGC(1/-1)
4612 GAATGC
BspLU11I 575 ACATGT A^CATGT
726 ACATGT
BsrBI 2516 GAGCGG CCGCTC(-3/-3)
6017 GAGCGG
BsrFI 671 ACCGGC [GA]^CCGG[CT]
2375 GCCGGC
BssSI 2238 CACGAG CACGAG(-5/-1)
5479 CTCGTG
BstAPI 201 GCAACTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
847 GCAACCAATGC
BstUI 5 CGCG CG^CG
5513 CGCG
CelII 148 GCTCAGC GC^T[ACGT]AGC
2640 GCTGAGC
Cfr10I 671 ACCGGC [GA]^CCGG[CT]
2375 GCCGGC
DrdI 2884 GACCCTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6130 GACTTGTATGTC
HincII 358 GTCAAC GT[CT]^[GA]AC
3238 GTCAAC
HindII 358 GTCAAC GT[CT]^[GA]AC
3238 GTCAAC
MluNI 296 TGGCCA TGG^CCA
5743 TGGCCA
MscI 296 TGGCCA TGG^CCA
5743 TGGCCA
MvnI 5 CGCG CG^CG
5513 CGCG
PflMI 1737 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2593 CCAGAATCTGG
PvuII 2526 CAGCTG CAG^CTG
3509 CAGCTG
SspI 4620 AATATT AAT^ATT
6189 AATATT
Van91I 1737 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2593 CCAGAATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccIII AciI AcsI AcyI AfaI
AflIII AluI AlwI AocI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbsI BbvI BclI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspEI
BspHI BspMI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MroI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RcaI RsaI SacI Sau3AI Sau96I
ScaI ScrFI SexAI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI