##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccIII AgeI Aor51HI ApaI AscI
AviII AvrII BalI BamHI BbrPI
BciVI BlnI BsaAI BsaBI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BsrFI BsrGI BssSI BstBI Cfr10I
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I FseI FspI HincII
HindII HpaI MamI MluI MluNI
MroI MscI NaeI NgoMIV NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PvuI
RsrII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SnaBI Sse8387I
SspBI StuI SwaI Van91I XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 33 GACGTC GACGT^C
Acc65I 4672 GGTACC G^GTACC
AclI 3648 AACGTT AA^CGTT
AflII 2673 CTTAAG C^TTAAG
Asp700I 575 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4672 GGTACC G^GTACC
BbeI 203 GGCGCC GGCGC^C
BbvCI 238 CCTCAGC CCTCAGC(-5/-2)
BcgI 293 GCAGGGATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2673 CTTAAG C^TTAAG
BglI 1702 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2603 AGATCT A^GATCT
BlpI 529 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 529 GCTTAGC GC^T[ACGT]AGC
BsaWI 468 ACCGGA [AT]^CCGG[AT]
BsmI 1455 GCATTC GAATGC(1/-1)
BspHI 4288 TCATGA T^CATGA
BspLU11I 4622 ACATGT A^CATGT
BspMI 550 ACCTGC ACCTGC(4/8)
BssHII 160 GCGCGC G^CGCGC
Bst1107I 2744 GTATAC GTA^TAC
BstEII 1384 GGTTACC G^GT[ACGT]ACC
BstPI 1384 GGTTACC G^GT[ACGT]ACC
BstZ17I 2744 GTATAC GTA^TAC
CelII 529 GCTTAGC GC^T[ACGT]AGC
DraII 1739 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 1498 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 178 TGGCCG [CT]^GGCC[GA]
EcoNI 4760 CCTTTGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1739 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1384 GGTTACC G^GT[ACGT]ACC
EcoT22I 3694 ATGCAT ATGCA^T
HindIII 980 AAGCTT A^AGCTT
KasI 203 GGCGCC G^GCGCC
KpnI 4672 GGTACC GGTAC^C
KspI 181 CCGCGG CCGC^GG
MfeI 3390 CAATTG C^AATTG
MunI 3390 CAATTG C^AATTG
NarI 203 GGCGCC GG^CGCC
NdeI 2486 CATATG CA^TATG
NheI 1301 GCTAGC G^CTAGC
NsiI 3694 ATGCAT ATGCA^T
PpuMI 1739 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 467 GACCGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3648 AACGTT AA^CGTT
PstI 4384 CTGCAG CTGCA^G
RcaI 4288 TCATGA T^CATGA
SacI 2138 GAGCTC GAGCT^C
SacII 181 CCGCGG CCGC^GG
SfoI 203 GGCGCC GGC^GCC
SpeI 3161 ACTAGT A^CTAGT
XbaI 4121 TCTAGA T^CTAGA
XmnI 575 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 2819 ACACGT A^C[GA][CT]GT
4622 ACATGT
AhdI 672 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2678 GACCTGTCGTC
AlwNI 855 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
2868 CAGAATCTG
AocI 1183 CCTCAGG CC^T[ACGT]AGG
1763 CCTGAGG
AseI 635 ATTAAT AT^TAAT
3338 ATTAAT
AsnI 635 ATTAAT AT^TAAT
3338 ATTAAT
AspEI 672 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2678 GACCTGTCGTC
BanI 203 GGCGCC G^G[CT][GA]CC
4672 GGTACC
BclI 330 TGATCA T^GATCA
3297 TGATCA
BsrBI 96 CCGCTC CCGCTC(-3/-3)
1476 CCGCTC
BstXI 1636 CCATGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2514 CCAGCGAATTGG
Bsu36I 1183 CCTCAGG CC^T[ACGT]AGG
1763 CCTGAGG
Eam1105I 672 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2678 GACCTGTCGTC
Eco81I 1183 CCTCAGG CC^T[ACGT]AGG
1763 CCTGAGG
EcoRI 579 GAATTC G^AATTC
3965 GAATTC
EcoRV 734 GATATC GAT^ATC
756 GATATC
FbaI 330 TGATCA T^GATCA
3297 TGATCA
NcoI 1365 CCATGG C^CATGG
1636 CCATGG
PshBI 635 ATTAAT AT^TAAT
3338 ATTAAT
SmaI 263 CCCGGG CCC^GGG
281 CCCGGG
XmaCI 263 CCCGGG C^CCGGG
281 CCCGGG
XmaI 263 CCCGGG C^CCGGG
281 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI ApaLI
ApoI AspHI AspI AvaI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DsaI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PleI
PvuII RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SphI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XcmI XhoII