##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

AccIII      AgeI        Aor51HI     ApaI        AscI        
AviII       AvrII       BalI        BamHI       BbrPI       
BciVI       BlnI        BsaAI       BsaBI       BseAI       
BsiEI       BsiWI       Bsp1407I    BspDI       BspEI       
BsrFI       BsrGI       BssSI       BstBI       Cfr10I      
ClaI        CpoI        DrdI        EagI        EclXI       
Eco47III    Eco52I      FseI        FspI        HincII      
HindII      HpaI        MamI        MluI        MluNI       
MroI        MscI        NaeI        NgoMIV      NotI        
NruI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PvuI        
RsrII       SalI        SapI        SbfI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       Sse8387I    
SspBI       StuI        SwaI        Van91I      XhoI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AatII          33  GACGTC               GACGT^C
Acc65I       4672  GGTACC               G^GTACC
AclI         3648  AACGTT               AA^CGTT
AflII        2673  CTTAAG               C^TTAAG
Asp700I       575  GAAGGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      4672  GGTACC               G^GTACC
BbeI          203  GGCGCC               GGCGC^C
BbvCI         238  CCTCAGC              CCTCAGC(-5/-2)
BcgI          293  GCAGGGATGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         2673  CTTAAG               C^TTAAG
BglI         1702  GCCTGGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        2603  AGATCT               A^GATCT
BlpI          529  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I      529  GCTTAGC              GC^T[ACGT]AGC
BsaWI         468  ACCGGA               [AT]^CCGG[AT]
BsmI         1455  GCATTC               GAATGC(1/-1)
BspHI        4288  TCATGA               T^CATGA
BspLU11I     4622  ACATGT               A^CATGT
BspMI         550  ACCTGC               ACCTGC(4/8)
BssHII        160  GCGCGC               G^CGCGC
Bst1107I     2744  GTATAC               GTA^TAC
BstEII       1384  GGTTACC              G^GT[ACGT]ACC
BstPI        1384  GGTTACC              G^GT[ACGT]ACC
BstZ17I      2744  GTATAC               GTA^TAC
CelII         529  GCTTAGC              GC^T[ACGT]AGC
DraII        1739  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DraIII       1498  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          178  TGGCCG               [CT]^GGCC[GA]
EcoNI        4760  CCTTTGTTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1739  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      1384  GGTTACC              G^GT[ACGT]ACC
EcoT22I      3694  ATGCAT               ATGCA^T
HindIII       980  AAGCTT               A^AGCTT
KasI          203  GGCGCC               G^GCGCC
KpnI         4672  GGTACC               GGTAC^C
KspI          181  CCGCGG               CCGC^GG
MfeI         3390  CAATTG               C^AATTG
MunI         3390  CAATTG               C^AATTG
NarI          203  GGCGCC               GG^CGCC
NdeI         2486  CATATG               CA^TATG
NheI         1301  GCTAGC               G^CTAGC
NsiI         3694  ATGCAT               ATGCA^T
PpuMI        1739  GGGTCCT              [GA]G^G[AT]CC[CT]
PshAI         467  GACCGGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     3648  AACGTT               AA^CGTT
PstI         4384  CTGCAG               CTGCA^G
RcaI         4288  TCATGA               T^CATGA
SacI         2138  GAGCTC               GAGCT^C
SacII         181  CCGCGG               CCGC^GG
SfoI          203  GGCGCC               GGC^GCC
SpeI         3161  ACTAGT               A^CTAGT
XbaI         4121  TCTAGA               T^CTAGA
XmnI          575  GAAGGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AflIII       2819  ACACGT               A^C[GA][CT]GT
             4622  ACATGT              
AhdI          672  GACGAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2678  GACCTGTCGTC         
AlwNI         855  CAGGATCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2868  CAGAATCTG           
AocI         1183  CCTCAGG              CC^T[ACGT]AGG
             1763  CCTGAGG             
AseI          635  ATTAAT               AT^TAAT
             3338  ATTAAT              
AsnI          635  ATTAAT               AT^TAAT
             3338  ATTAAT              
AspEI         672  GACGAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2678  GACCTGTCGTC         
BanI          203  GGCGCC               G^G[CT][GA]CC
             4672  GGTACC              
BclI          330  TGATCA               T^GATCA
             3297  TGATCA              
BsrBI          96  CCGCTC               CCGCTC(-3/-3)
             1476  CCGCTC              
BstXI        1636  CCATGGTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2514  CCAGCGAATTGG        
Bsu36I       1183  CCTCAGG              CC^T[ACGT]AGG
             1763  CCTGAGG             
Eam1105I      672  GACGAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2678  GACCTGTCGTC         
Eco81I       1183  CCTCAGG              CC^T[ACGT]AGG
             1763  CCTGAGG             
EcoRI         579  GAATTC               G^AATTC
             3965  GAATTC              
EcoRV         734  GATATC               GAT^ATC
              756  GATATC              
FbaI          330  TGATCA               T^GATCA
             3297  TGATCA              
NcoI         1365  CCATGG               C^CATGG
             1636  CCATGG              
PshBI         635  ATTAAT               AT^TAAT
             3338  ATTAAT              
SmaI          263  CCCGGG               CCC^GGG
              281  CCCGGG              
XmaCI         263  CCCGGG               C^CCGGG
              281  CCCGGG              
XmaI          263  CCCGGG               C^CCGGG
              281  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AluI        Alw44I      AlwI        ApaLI       
ApoI        AspHI       AspI        AvaI        AvaII       
BanII       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BsrDI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DsaI        EarI        EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflFI       PleI        
PvuII       RsaI        Sau3AI      Sau96I      ScaI        
ScrFI       SfaNI       SfcI        SmlI        SphI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     XcmI        XhoII