##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AflII Alw44I ApaLI AscI
AseI AsnI AspI AvrII BclI
BfrI BglII BlnI BsaBI BsiWI
BsmI BspDI BssHII BstBI ClaI
CpoI DraI DrdI EagI EclXI
Eco52I EcoRI EcoT22I FbaI HindIII
HpaI MamI MfeI MluI MunI
NdeI NotI NruI NsiI NspV
PacI PflFI PmeI PshBI PvuI
RsrII SapI SfuI SgrAI SpeI
SphI SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 1242 GGTACC G^GTACC
AccII 2831 CGCG CG^CG
AclI 2612 AACGTT AA^CGTT
AcsI 4697 AAATTT [GA]^AATT[CT]
AgeI 3714 ACCGGT A^CCGGT
Aor51HI 1430 AGCGCT AGC^GCT
ApoI 4697 AAATTT [GA]^AATT[CT]
Asp718I 1242 GGTACC G^GTACC
AviII 1809 TGCGCA TGC^GCA
BamHI 5260 GGATCC G^GATCC
BbrPI 1397 CACGTG CAC^GTG
BsiEI 3235 CGACCG CG[GA][CT]^CG
BsmBI 3674 GAGACG CGTCTC(1/5)
Bsp1407I 5553 TGTACA T^GTACA
BspHI 3826 TCATGA T^CATGA
BsrGI 5553 TGTACA T^GTACA
BssSI 501 CTCGTG CACGAG(-5/-1)
Bst1107I 5616 GTATAC GTA^TAC
BstUI 2831 CGCG CG^CG
BstZ17I 5616 GTATAC GTA^TAC
Eco47III 1430 AGCGCT AGC^GCT
EcoRV 3040 GATATC GAT^ATC
FseI 5108 GGCCGGCC GGCCGG^CC
FspI 1809 TGCGCA TGC^GCA
HincII 2871 GTCGAC GT[CT]^[GA]AC
HindII 2871 GTCGAC GT[CT]^[GA]AC
KpnI 1242 GGTACC GGTAC^C
KspI 2830 CCGCGG CCGC^GG
MseI 5591 TTAA T^TAA
MvnI 2831 CGCG CG^CG
PaeR7I 2922 CTCGAG C^TCGAG
PinAI 3714 ACCGGT A^CCGGT
PmaCI 1397 CACGTG CAC^GTG
PmlI 1397 CACGTG CAC^GTG
PshAI 3953 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2612 AACGTT AA^CGTT
RcaI 3826 TCATGA T^CATGA
SacI 1982 GAGCTC GAGCT^C
SacII 2830 CCGCGG CCGC^GG
SalI 2871 GTCGAC G^TCGAC
ScaI 4047 AGTACT AGT^ACT
SnaBI 2627 TACGTA TAC^GTA
SspBI 5553 TGTACA T^GTACA
Tru9I 5591 TTAA T^TAA
XhoI 2922 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 2871 GTCGAC GT^[AC][GT]AC
5616 GTATAC
AccIII 1261 TCCGGA T^CCGGA
2850 TCCGGA
AhdI 3074 GACGTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3086 GACCTGGCGTC
AspEI 3074 GACGTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3086 GACCTGGCGTC
AspHI 1982 GAGCTC G[AT]GC[AT]^C
2320 GAGCAC
BbeI 1051 GGCGCC GGCGC^C
5419 GGCGCC
BciVI 3511 GGATAC GTATCC(6/5)
4030 GTATCC
BlpI 2240 GCTGAGC GC^T[ACGT]AGC
4362 GCTCAGC
Bpu1102I 2240 GCTGAGC GC^T[ACGT]AGC
4362 GCTCAGC
BseAI 1261 TCCGGA T^CCGGA
2850 TCCGGA
BsiHKAI 1982 GAGCTC G[AT]GC[AT]^C
2320 GAGCAC
BspEI 1261 TCCGGA T^CCGGA
2850 TCCGGA
BspLU11I 546 ACATGT A^CATGT
3562 ACATGT
CelII 2240 GCTGAGC GC^T[ACGT]AGC
4362 GCTCAGC
DraIII 206 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
4857 CACCAGGTG
Eam1105I 3074 GACGTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3086 GACCTGGCGTC
KasI 1051 GGCGCC G^GCGCC
5419 GGCGCC
MroI 1261 TCCGGA T^CCGGA
2850 TCCGGA
NarI 1051 GGCGCC GG^CGCC
5419 GGCGCC
SbfI 4385 CCTGCAGG CCTGCA^GG
4538 CCTGCAGG
SexAI 2749 ACCAGGT A^CC[AT]GGT
4858 ACCAGGT
SfiI 2946 GGCCCATGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5103 GGCCTGGCCGGCC
SfoI 1051 GGCGCC GGC^GCC
5419 GGCGCC
Sse8387I 4385 CCTGCAGG CCTGCA^GG
4538 CCTGCAGG
Tsp509I 246 AATT ^AATT
4698 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AlwNI AocI ApaI Asp700I
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcgI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NaeI NciI NcoI
NdeII NgoMIV NheI NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI XmnI