##################################
# NO CUT ENZYMES (   40 enzymes) #
##################################

AccIII      AclI        AhdI        AscI        AseI        
AsnI        Asp700I     AspEI       AviII       BseAI       
BsiWI       BspEI       BssHII      Bst1107I    BstZ17I     
CpoI        Eam1105I    EcoT22I     FseI        FspI        
HpaI        MroI        NotI        NruI        NsiI        
PacI        PmeI        PshBI       Psp1406I    PvuI        
RsrII       SalI        SbfI        SfiI        SgrAI       
SnaBI       Sse8387I    SwaI        XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AatII        4739  GACGTC               GACGT^C
Acc65I       2166  GGTACC               G^GTACC
AflII        9200  CTTAAG               C^TTAAG
AgeI         5995  ACCGGT               A^CCGGT
Asp718I      2166  GGTACC               G^GTACC
AspI        10158  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        5494  CCTAGG               C^CTAGG
BbvCI         437  GCTGAGG              CCTCAGC(-5/-2)
BfrI         9200  CTTAAG               C^TTAAG
BlnI         5494  CCTAGG               C^CTAGG
BsaBI        2833  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI        5442  ATCGAT               AT^CGAT
BssSI        6800  CTCGTG               CACGAG(-5/-1)
BstBI        4206  TTCGAA               TT^CGAA
BstEII       5701  GGTAACC              G^GT[ACGT]ACC
BstPI        5701  GGTAACC              G^GT[ACGT]ACC
ClaI         5442  ATCGAT               AT^CGAT
DraI          931  TTTAAA               TTT^AAA
DrdI         2864  GACACTCTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      5701  GGTAACC              G^GT[ACGT]ACC
EcoRV        9783  GATATC               GAT^ATC
KpnI         2166  GGTACC               GGTAC^C
MamI         2833  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI        10338  CAATTG               C^AATTG
MluI         9748  ACGCGT               A^CGCGT
MunI        10338  CAATTG               C^AATTG
NdeI         8006  CATATG               CA^TATG
NspV         4206  TTCGAA               TT^CGAA
PflFI       10158  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        5995  ACCGGT               A^CCGGT
ScaI         7026  AGTACT               AGT^ACT
SfuI         4206  TTCGAA               TT^CGAA
SpeI         8973  ACTAGT               A^CTAGT
SspI         2088  AATATT               AAT^ATT
Tth111I     10158  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AccI         6518  GTAGAC               GT^[AC][GT]AC
            10752  GTAGAC              
Aor51HI       190  AGCGCT               AGC^GCT
             2133  AGCGCT              
BamHI        2785  GGATCC               G^GATCC
             6163  GGATCC              
BsrBI       10977  CCGCTC               CCGCTC(-3/-3)
              320  GAGCGG              
Eco47III      190  AGCGCT               AGC^GCT
             2133  AGCGCT              
HindIII      1943  AAGCTT               A^AGCTT
            10152  AAGCTT              
NheI         6206  GCTAGC               G^CTAGC
             9494  GCTAGC              
PshAI        3596  GACAGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             4520  GACCAGAGTC          
SexAI        2301  ACCTGGT              A^CC[AT]GGT
            10159  ACCAGGT             
SmaI          172  CCCGGG               CCC^GGG
            10381  CCCGGG              
XmaCI         172  CCCGGG               C^CCGGG
            10381  CCCGGG              
XmaI          172  CCCGGG               C^CCGGG
            10381  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  171 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        Alw44I      AlwI        AlwNI       
AocI        ApaI        ApaLI       ApoI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbeI        BbrPI       BbsI        BbvI        BcgI        
BciVI       BclI        BcnI        BfaI        BglI        
BglII       BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaHI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspHI       
BspLU11I    BspMI       BsrDI       BsrFI       BsrGI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EagI        EarI        EclXI       Eco52I      Eco81I      
EcoNI       EcoO109I    EcoRI       EcoRII      EcoT14I     
FauI        FbaI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PaeR7I      PflMI       PleI        PmaCI       PmlI        
PpuMI       PstI        PvuII       RcaI        RsaI        
SacI        SacII       SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfoI        SmlI        
SphI        SspBI       StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoI        
XhoII