##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AccIII AclI AflII AscI AseI
AsnI Asp700I BbeI BfrI BseAI
BspEI Bst1107I BstBI BstZ17I CpoI
EagI EclXI Eco52I EcoT22I FseI
HindIII HpaI KasI KspI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PmeI PshAI PshBI
Psp1406I RsrII SacII SbfI ScaI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 782 GGTACC G^GTACC
AgeI 2709 ACCGGT A^CCGGT
Alw44I 4502 GTGCAC G^TGCAC
Aor51HI 4570 AGCGCT AGC^GCT
ApaLI 4502 GTGCAC G^TGCAC
Asp718I 782 GGTACC G^GTACC
AviII 2049 TGCGCA TGC^GCA
AvrII 5916 CCTAGG C^CTAGG
BciVI 5076 GTATCC GTATCC(6/5)
BclI 2412 TGATCA T^GATCA
BglII 2130 AGATCT A^GATCT
BlnI 5916 CCTAGG C^CTAGG
BsaBI 5699 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2691 CGATCG CG[GA][CT]^CG
BsiWI 5577 CGTACG C^GTACG
BsmI 5132 GCATTC GAATGC(1/-1)
BspDI 2288 ATCGAT AT^CGAT
BssHII 3394 GCGCGC G^CGCGC
BssSI 2165 CACGAG CACGAG(-5/-1)
ClaI 2288 ATCGAT AT^CGAT
DraI 6261 TTTAAA TTT^AAA
DraIII 900 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2732 GACCTCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4570 AGCGCT AGC^GCT
EcoRI 5357 GAATTC G^AATTC
FbaI 2412 TGATCA T^GATCA
FspI 2049 TGCGCA TGC^GCA
KpnI 782 GGTACC GGTAC^C
MamI 5699 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 5892 CATATG CA^TATG
PaeR7I 1802 CTCGAG C^TCGAG
PinAI 2709 ACCGGT A^CCGGT
PvuI 2691 CGATCG CGAT^CG
SapI 1037 GAAGAGC GCTCTTC(1/4)
SfiI 5155 GGCCAGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 5316 AATATT AAT^ATT
XhoI 1802 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 956 GAATTT [GA]^AATT[CT]
5357 GAATTC
ApoI 956 GAATTT [GA]^AATT[CT]
5357 GAATTC
AspI 4986 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5426 GACCTGGTC
BamHI 535 GGATCC G^GATCC
2430 GGATCC
BcgI 1240 CGACAGGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3559 CGAGGCCGCTGC
BlpI 2537 GCTGAGC GC^T[ACGT]AGC
3385 GCTGAGC
Bpu1102I 2537 GCTGAGC GC^T[ACGT]AGC
3385 GCTGAGC
Bsp1407I 2796 TGTACA T^GTACA
5367 TGTACA
BsrBI 44 CCGCTC CCGCTC(-3/-3)
3937 CCGCTC
BsrGI 2796 TGTACA T^GTACA
5367 TGTACA
CelII 2537 GCTGAGC GC^T[ACGT]AGC
3385 GCTGAGC
EcoRV 191 GATATC GAT^ATC
1179 GATATC
MseI 6238 TTAA T^TAA
6262 TTAA
PflFI 4986 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5426 GACCTGGTC
SalI 162 GTCGAC G^TCGAC
3989 GTCGAC
SmaI 3464 CCCGGG CCC^GGG
3960 CCCGGG
SspBI 2796 TGTACA T^GTACA
5367 TGTACA
Tru9I 6238 TTAA T^TAA
6262 TTAA
Tth111I 4986 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5426 GACCTGGTC
XbaI 3232 TCTAGA T^CTAGA
5666 TCTAGA
XmaCI 3464 CCCGGG C^CCGGG
3960 CCCGGG
XmaI 3464 CCCGGG C^CCGGG
3960 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 146 enzymes) #
#########################################
AatII AccI AccII AciI AcyI
AfaI AflIII AhdI AluI AlwI
AlwNI AocI ApaI AspEI AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspHI BspLU11I BspMI BsrDI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eam1105I EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI SphI StuI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII