##################################
# NO CUT ENZYMES (   62 enzymes) #
##################################

AatII       AccI        AclI        AgeI        Aor51HI     
AscI        AseI        AsnI        AspI        BbrPI       
BclI        BsaBI       BsiEI       BsiWI       BspDI       
BspHI       BssHII      Bst1107I    BstBI       BstZ17I     
ClaI        CpoI        DrdI        EagI        EclXI       
Eco47III    Eco52I      EcoRV       FbaI        FseI        
HincII      HindII      HindIII     HpaI        MamI        
NheI        NotI        NruI        NspV        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SalI        SbfI        ScaI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspI        SwaI        
Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

Acc65I       2064  GGTACC               G^GTACC
Asp718I      2064  GGTACC               G^GTACC
AviII         711  TGCGCA               TGC^GCA
AvrII         377  CCTAGG               C^CTAGG
BcgI         6378  CGAGCCTGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        5408  GTATCC               GTATCC(6/5)
BglII        6726  AGATCT               A^GATCT
BlnI          377  CCTAGG               C^CTAGG
BsaAI        1593  TACGTG               [CT]AC^GT[GA]
BsmBI        1669  CGTCTC               CGTCTC(1/5)
BspLU11I     5416  ACATGT               A^CATGT
BssSI        2137  CACGAG               CACGAG(-5/-1)
DraIII       3686  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI        3039  CCTGAGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      6960  ATGCAT               ATGCA^T
FspI          711  TGCGCA               TGC^GCA
KpnI         2064  GGTACC               GGTAC^C
KspI         1278  CCGCGG               CCGC^GG
MfeI          354  CAATTG               C^AATTG
MluI            4  ACGCGT               A^CGCGT
MunI          354  CAATTG               C^AATTG
NdeI         4428  CATATG               CA^TATG
NsiI         6960  ATGCAT               ATGCA^T
PacI         6340  TTAATTAA             TTAAT^TAA
SacII        1278  CCGCGG               CCGC^GG
SexAI        6390  ACCTGGT              A^CC[AT]GGT
SfiI         1084  GGCCTGCTTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI         3776  AGGCCT               AGG^CCT
XbaI         3584  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccIII        265  TCCGGA               T^CCGGA
             2564  TCCGGA              
AcyI         1350  GGCGCC               G[GA]^CG[CT]C
             3144  GGCGCC              
AhdI         1493  GACCCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3705  GACCAGCAGTC         
AspEI        1493  GACCCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3705  GACCAGCAGTC         
BbeI         1350  GGCGCC               GGCGC^C
             3144  GGCGCC              
BlpI         3917  GCTCAGC              GC^T[ACGT]AGC
             5075  GCTGAGC             
Bpu1102I     3917  GCTCAGC              GC^T[ACGT]AGC
             5075  GCTGAGC             
BsaHI        1350  GGCGCC               G[GA]^CG[CT]C
             3144  GGCGCC              
BsaI         2777  GAGACC               GGTCTC(1/5)
             1837  GGTCTC              
BseAI         265  TCCGGA               T^CCGGA
             2564  TCCGGA              
BspEI         265  TCCGGA               T^CCGGA
             2564  TCCGGA              
BsrBI        1973  CCGCTC               CCGCTC(-3/-3)
               43  GAGCGG              
BstEII       1282  GGTCACC              G^GT[ACGT]ACC
             3804  GGTGACC             
BstPI        1282  GGTCACC              G^GT[ACGT]ACC
             3804  GGTGACC             
CelII        3917  GCTCAGC              GC^T[ACGT]AGC
             5075  GCTGAGC             
Eam1105I     1493  GACCCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3705  GACCAGCAGTC         
EcoO65I      1282  GGTCACC              G^GT[ACGT]ACC
             3804  GGTGACC             
EcoRI        6139  GAATTC               G^AATTC
             6734  GAATTC              
HgaI            3  GACGC                GACGC(5/10)
             4500  GCGTC               
Hin1I        1350  GGCGCC               G[GA]^CG[CT]C
             3144  GGCGCC              
KasI         1350  GGCGCC               G^GCGCC
             3144  GGCGCC              
MroI          265  TCCGGA               T^CCGGA
             2564  TCCGGA              
NaeI           23  GCCGGC               GCC^GGC
             7124  GCCGGC              
NarI         1350  GGCGCC               GG^CGCC
             3144  GGCGCC              
NcoI          942  CCATGG               C^CATGG
             4867  CCATGG              
NgoMIV         23  GCCGGC               G^CCGGC
             7124  GCCGGC              
PshAI        2511  GACCAGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2568  GACTCTCGTC          
SfoI         1350  GGCGCC               GGC^GCC
             3144  GGCGCC              
TaqI          442  TCGA                 T^CGA
             3507  TCGA                
TthHB8I       442  TCGA                 T^CGA
             3507  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (  137 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflII       
AflIII      AluI        Alw44I      AlwI        AlwNI       
AocI        ApaI        ApaLI       ApoI        Asp700I     
AspHI       AvaI        AvaII       BalI        BamHI       
BanI        BanII       BbsI        BbvCI       BbvI        
BcnI        BfaI        BfrI        BglI        BmyI        
BpmI        BpuAI       BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspMI       BsrDI       BsrFI       BsrGI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EaeI        EarI        Eco81I      
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        SmlI        SphI        SspBI       StyI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Van91I      XcmI        XhoII       XmaCI       
XmaI        XmnI