##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI AhdI AocI AscI AspEI
BbeI BbrPI BbvCI BfrI BsaHI
BsaWI BseAI BsgI BsiWI BsmBI
BspDI BspEI BsrBI BsrFI BssHII
BssSI BstBI Bsu36I Cfr10I ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco52I Eco81I EcoRV FauI FseI
Hin1I HindIII KasI KspI MluI
MroI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 729 GGTACC G^GTACC
AflIII 4047 ACATGT A^C[GA][CT]GT
Alw44I 3947 GTGCAC G^TGCAC
Aor51HI 563 AGCGCT AGC^GCT
ApaI 570 GGGCCC GGGCC^C
ApaLI 3947 GTGCAC G^TGCAC
Asp718I 729 GGTACC G^GTACC
AspI 1625 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 35 CTCGGG C^[CT]CG[GA]G
AviII 3086 TGCGCA TGC^GCA
BalI 3116 TGGCCA TGG^CCA
BamHI 1933 GGATCC G^GATCC
BcgI 3057 GCACAAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1323 TGATCA T^GATCA
BglI 1469 GCCTTACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3001 TACGTG [CT]AC^GT[GA]
BsiEI 2790 CGGTCG CG[GA][CT]^CG
BsoBI 35 CTCGGG C^[CT]CG[GA]G
BspHI 6 TCATGA T^CATGA
BspLU11I 4047 ACATGT A^CATGT
Bst1107I 969 GTATAC GTA^TAC
BstEII 4659 GGTTACC G^GT[ACGT]ACC
BstPI 4659 GGTTACC G^GT[ACGT]ACC
BstZ17I 969 GTATAC GTA^TAC
DrdI 5922 GACCAGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 563 AGCGCT AGC^GCT
EcoO65I 4659 GGTTACC G^GT[ACGT]ACC
EcoRI 5550 GAATTC G^AATTC
FbaI 1323 TGATCA T^GATCA
FspI 3086 TGCGCA TGC^GCA
HaeII 563 AGCGCT [GA]GCGC^[CT]
HgaI 1176 GCGTC GACGC(5/10)
KpnI 729 GGTACC GGTAC^C
MluNI 3116 TGGCCA TGG^CCA
MscI 3116 TGGCCA TGG^CCA
PflFI 1625 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3175 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 6 TCATGA T^CATGA
SacI 1373 GAGCTC GAGCT^C
SalI 2792 GTCGAC G^TCGAC
SexAI 1626 ACCTGGT A^CC[AT]GGT
Tth111I 1625 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3175 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2221 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 248 CGCG CG^CG
2440 CGCG
AseI 2307 ATTAAT AT^TAAT
3701 ATTAAT
AsnI 2307 ATTAAT AT^TAAT
3701 ATTAAT
AvrII 3156 CCTAGG C^CTAGG
5875 CCTAGG
BanII 570 GGGCCC G[GA]GC[CT]^C
1373 GAGCTC
BbsI 2800 GAAGAC GAAGAC(2/6)
5559 GTCTTC
BciVI 4373 GGATAC GTATCC(6/5)
1056 GTATCC
BcnI 2078 CCCGG CC^[GC]GG
3150 CCCGG
BlnI 3156 CCTAGG C^CTAGG
5875 CCTAGG
BpuAI 2800 GAAGAC GAAGAC(2/6)
5559 GTCTTC
BsaBI 511 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1934 GATCCTCATC
BsmI 3185 GAATGC GAATGC(1/-1)
3443 GAATGC
BstUI 248 CGCG CG^CG
2440 CGCG
BstXI 2943 CCAGAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2996 CCAATTACGTGG
EcoNI 376 CCTGAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3163 CCTATTGGAGG
EcoT22I 851 ATGCAT ATGCA^T
4206 ATGCAT
HapII 2079 CCGG C^CGG
3151 CCGG
HpaI 825 GTTAAC GTT^AAC
5969 GTTAAC
HpaII 2079 CCGG C^CGG
3151 CCGG
MaeII 3002 ACGT A^CGT
4401 ACGT
MamI 511 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1934 GATCCTCATC
MspI 2079 CCGG C^CGG
3151 CCGG
MvnI 248 CGCG CG^CG
2440 CGCG
NciI 2078 CCCGG CC^[GC]GG
3150 CCCGG
NsiI 851 ATGCAT ATGCA^T
4206 ATGCAT
PpuMI 3159 AGGACCT [GA]G^G[AT]CC[CT]
5662 GGGTCCT
PshBI 2307 ATTAAT AT^TAAT
3701 ATTAAT
ScaI 1299 AGTACT AGT^ACT
4728 AGTACT
SmlI 2204 CTCAAG C^T[CT][GA]AG
5888 CTTGAG
SphI 1283 GCATGC GCATG^C
1868 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BanI BbvI BfaI BglII
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HhaI HinP1I HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MunI MvaI
MwoI NcoI NdeI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI