##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

Acc65I      AclI        AflII       AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       BamHI       
BciVI       BfrI        BglII       BsaBI       BsiWI       
Bsp1407I    BspDI       BspHI       BspLU11I    BsrFI       
BsrGI       BssHII      Bst1107I    BstBI       BstZ17I     
Cfr10I      ClaI        DraI        DraIII      DrdI        
Eam1105I    Eco47III    EcoNI       EcoRV       FseI        
HincII      HindII      HpaI        KpnI        KspI        
MamI        MfeI        MluI        MunI        NaeI        
NdeI        NgoMIV      NotI        NruI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AatII        3218  GACGTC               GACGT^C
AccI         2852  GTCTAC               GT^[AC][GT]AC
AccIII       1830  TCCGGA               T^CCGGA
ApaI         1869  GGGCCC               GGGCC^C
Asp700I       532  GAACAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         2950  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        1882  CACGTG               CAC^GTG
BcgI          955  CGAGGCAGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          255  TGATCA               T^GATCA
BlpI         2158  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2158  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1882  CACGTG               [CT]AC^GT[GA]
BseAI        1830  TCCGGA               T^CCGGA
BsiEI        2313  CGGCCG               CG[GA][CT]^CG
BsmBI         554  CGTCTC               CGTCTC(1/5)
BsmI          240  GCATTC               GAATGC(1/-1)
BspEI        1830  TCCGGA               T^CCGGA
BspMI        1464  ACCTGC               ACCTGC(4/8)
BsrDI        1759  GCAATG               GCAATG(2/0)
BstYI        1743  AGATCC               [GA]^GATC[CT]
CelII        2158  GCTGAGC              GC^T[ACGT]AGC
CpoI         2657  CGGACCG              CG^G[AT]CCG
EagI         2313  CGGCCG               C^GGCCG
EclXI        2313  CGGCCG               C^GGCCG
Eco52I       2313  CGGCCG               C^GGCCG
EcoRI        1846  GAATTC               G^AATTC
EcoT22I        89  ATGCAT               ATGCA^T
FbaI          255  TGATCA               T^GATCA
HindIII      1063  AAGCTT               A^AGCTT
MflI         1743  AGATCC               [GA]^GATC[CT]
MroI         1830  TCCGGA               T^CCGGA
NsiI           89  ATGCAT               ATGCA^T
PflFI        2950  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1882  CACGTG               CAC^GTG
PmlI         1882  CACGTG               CAC^GTG
RsrII        2657  CGGACCG              CG^G[AT]CCG
SphI           91  GCATGC               GCATG^C
Tth111I      2950  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII        1743  AGATCC               [GA]^GATC[CT]
XmnI          532  GAACAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcsI         1483  GAATTT               [GA]^AATT[CT]
             1846  GAATTC              
AocI           40  CCTCAGG              CC^T[ACGT]AGG
              172  CCTCAGG             
ApoI         1483  GAATTT               [GA]^AATT[CT]
             1846  GAATTC              
AviII         786  TGCGCA               TGC^GCA
             1788  TGCGCA              
AvrII        2902  CCTAGG               C^CTAGG
             2933  CCTAGG              
BbeI         1904  GGCGCC               GGCGC^C
             2152  GGCGCC              
BlnI         2902  CCTAGG               C^CTAGG
             2933  CCTAGG              
BsaI         1524  GAGACC               GGTCTC(1/5)
             2796  GAGACC              
BssSI        2729  CACGAG               CACGAG(-5/-1)
              669  CTCGTG              
BstAPI        467  GCACCTGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2341  GCAACTGCTGC         
BstEII        778  GGTGACC              G^GT[ACGT]ACC
              935  GGTCACC             
BstPI         778  GGTGACC              G^GT[ACGT]ACC
              935  GGTCACC             
Bsu36I         40  CCTCAGG              CC^T[ACGT]AGG
              172  CCTCAGG             
Eco81I         40  CCTCAGG              CC^T[ACGT]AGG
              172  CCTCAGG             
EcoO65I       778  GGTGACC              G^GT[ACGT]ACC
              935  GGTCACC             
FspI          786  TGCGCA               TGC^GCA
             1788  TGCGCA              
KasI         1904  GGCGCC               G^GCGCC
             2152  GGCGCC              
NarI         1904  GGCGCC               GG^CGCC
             2152  GGCGCC              
NheI         2558  GCTAGC               G^CTAGC
             3053  GCTAGC              
SexAI         372  ACCAGGT              A^CC[AT]GGT
             3837  ACCAGGT             
SfoI         1904  GGCGCC               GGC^GCC
             2152  GGCGCC              


#########################################
# 3 OR MORE CUT ENZYMES (  116 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvCI       
BbvI        BcnI        BfaI        BglI        BmyI        
BpmI        BpuAI       BsaHI       BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrBI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XmaCI       
XmaI