##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AccIII AclI AflII AgeI AscI
AviII BbrPI BfrI BsaAI BsaBI
BseAI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII Bst1107I BstZ17I
ClaI CpoI DraIII EcoT22I FspI
HpaI MamI MfeI MroI MunI
NheI NotI NsiI PacI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SalI SbfI ScaI SexAI
SfiI SgrAI SnaBI SphI Sse8387I
SspBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Alw44I 707 GTGCAC G^TGCAC
Aor51HI 3108 AGCGCT AGC^GCT
ApaLI 707 GTGCAC G^TGCAC
AseI 1430 ATTAAT AT^TAAT
AsnI 1430 ATTAAT AT^TAAT
AspI 4805 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 5001 CCTAGG C^CTAGG
BclI 3936 TGATCA T^GATCA
BglII 4918 AGATCT A^GATCT
BlnI 5001 CCTAGG C^CTAGG
BlpI 4112 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4112 GCTCAGC GC^T[ACGT]AGC
BsaWI 4011 TCCGGT [AT]^CCGG[AT]
BsiEI 531 CGGCCG CG[GA][CT]^CG
BspHI 4365 TCATGA T^CATGA
BssSI 3144 CACGAG CACGAG(-5/-1)
BstEII 5046 GGTGACC G^GT[ACGT]ACC
BstPI 5046 GGTGACC G^GT[ACGT]ACC
CelII 4112 GCTCAGC GC^T[ACGT]AGC
DrdI 1647 GACAAAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 531 CGGCCG C^GGCCG
EclXI 531 CGGCCG C^GGCCG
Eco47III 3108 AGCGCT AGC^GCT
Eco52I 531 CGGCCG C^GGCCG
EcoNI 5180 CCTGAGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 5046 GGTGACC G^GT[ACGT]ACC
EcoRV 4304 GATATC GAT^ATC
FbaI 3936 TGATCA T^GATCA
FseI 528 GGCCGGCC GGCCGG^CC
HincII 1925 GTCAAC GT[CT]^[GA]AC
HindII 1925 GTCAAC GT[CT]^[GA]AC
KspI 758 CCGCGG CCGC^GG
MluI 711 ACGCGT A^CGCGT
NdeI 1505 CATATG CA^TATG
NruI 549 TCGCGA TCG^CGA
NspI 1752 GCATGT [GA]CATG^[CT]
PaeR7I 2406 CTCGAG C^TCGAG
PflFI 4805 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1337 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1430 ATTAAT AT^TAAT
RcaI 4365 TCATGA T^CATGA
SacII 758 CCGCGG CCGC^GG
SpeI 2638 ACTAGT A^CTAGT
SspI 5081 AATATT AAT^ATT
Tth111I 4805 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2406 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 2800 GGTACC G^GTACC
4770 GGTACC
AccI 2260 GTCTAC GT^[AC][GT]AC
2551 GTCTAC
Asp700I 3276 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4249 GAACTTCTTC
Asp718I 2800 GGTACC G^GTACC
4770 GGTACC
BamHI 2608 GGATCC G^GATCC
3390 GGATCC
BbeI 97 GGCGCC GGCGC^C
3651 GGCGCC
BbvCI 22 GCTGAGG CCTCAGC(-5/-2)
5296 GCTGAGG
BglI 415 GCCGACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
533 GCCGCTCCGGC
BsmBI 381 CGTCTC CGTCTC(1/5)
1175 CGTCTC
BsmI 359 GAATGC GAATGC(1/-1)
1703 GCATTC
BsrFI 529 GCCGGC [GA]^CCGG[CT]
3648 GCCGGC
BstBI 1008 TTCGAA TT^CGAA
5058 TTCGAA
BstXI 4059 CCAATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4680 CCAGAGGTTTGG
Cfr10I 529 GCCGGC [GA]^CCGG[CT]
3648 GCCGGC
DraI 5102 TTTAAA TTT^AAA
5426 TTTAAA
HindIII 2117 AAGCTT A^AGCTT
4661 AAGCTT
KasI 97 GGCGCC G^GCGCC
3651 GGCGCC
KpnI 2800 GGTACC GGTAC^C
4770 GGTACC
NaeI 529 GCCGGC GCC^GGC
3648 GCCGGC
NarI 97 GGCGCC GG^CGCC
3651 GGCGCC
NgoMIV 529 GCCGGC G^CCGGC
3648 GCCGGC
NspV 1008 TTCGAA TT^CGAA
5058 TTCGAA
SfoI 97 GGCGCC GGC^GCC
3651 GGCGCC
SfuI 1008 TTCGAA TT^CGAA
5058 TTCGAA
SmaI 509 CCCGGG CCC^GGG
2544 CCCGGG
StuI 3874 AGGCCT AGG^CCT
4513 AGGCCT
XmaCI 509 CCCGGG C^CCGGG
2544 CCCGGG
XmaI 509 CCCGGG C^CCGGG
2544 CCCGGG
XmnI 3276 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4249 GAACTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AatII AccII AciI AcsI AcyI
AfaI AflIII AhdI AluI AlwI
AlwNI AocI ApaI ApoI AspEI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcgI BciVI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI Eco81I EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII