##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccIII AclI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaII AviII
AvrII BamHI BbrPI BbvCI BcgI
BciVI BclI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BseAI BseRI
BsiHKAI BsiWI BsmBI BsmI BspDI
BspEI BspMI BsrBI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV FauI
FbaI FseI FspI HincII HindII
HpaI KpnI KspI MaeII MamI
MfeI MluI MroI MspA1I MunI
NcoI NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
SexAI SfuI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I Van91I
XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AflII 917 CTTAAG C^TTAAG
AgeI 83 ACCGGT A^CCGGT
ApaI 40 GGGCCC GGGCC^C
BalI 829 TGGCCA TGG^CCA
BanI 89 GGCGCC G^G[CT][GA]CC
BbeI 89 GGCGCC GGCGC^C
BbsI 938 GTCTTC GAAGAC(2/6)
BfrI 917 CTTAAG C^TTAAG
BglII 259 AGATCT A^GATCT
BpmI 109 CTGGAG CTGGAG(16/14)
BpuAI 938 GTCTTC GAAGAC(2/6)
BsaWI 83 ACCGGT [AT]^CCGG[AT]
BsgI 330 CTGCAC GTGCAG(16/14)
BsiEI 81 CGACCG CG[GA][CT]^CG
BsiYI 1152 CCTTTAAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1152 CCTTTAAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BstF5I 346 CATCC GGATG(2/0)
BstYI 259 AGATCT [GA]^GATC[CT]
DraII 39 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 930 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 829 TGGCCA [CT]^GGCC[GA]
EarI 141 CTCTTC CTCTTC(1/4)
EcoO109I 39 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 369 CCAAGG C^C[AT][AT]GG
EcoT22I 1027 ATGCAT ATGCA^T
FokI 346 CATCC GGATG(9/13)
HaeII 89 GGCGCC [GA]GCGC^[CT]
HgaI 807 GCGTC GACGC(5/10)
HindIII 237 AAGCTT A^AGCTT
KasI 89 GGCGCC G^GCGCC
Ksp632I 141 CTCTTC CTCTTC(1/4)
MflI 259 AGATCT [GA]^GATC[CT]
MluNI 829 TGGCCA TGG^CCA
MscI 829 TGGCCA TGG^CCA
MslI 1362 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 89 GGCGCC GG^CGCC
NdeI 228 CATATG CA^TATG
NsiI 1027 ATGCAT ATGCA^T
PinAI 83 ACCGGT A^CCGGT
ScaI 650 AGTACT AGT^ACT
SfcI 1331 CTGTAG C^T[GA][CT]AG
SfiI 32 GGCCCGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 89 GGCGCC GGC^GCC
SgrAI 91 CGCCGGCG C[GA]^CCGG[CT]G
StuI 797 AGGCCT AGG^CCT
StyI 369 CCAAGG C^C[AT][AT]GG
TaqI 157 TCGA T^CGA
Tsp45I 1053 GTGAC ^GT[GC]AC
TthHB8I 157 TCGA T^CGA
XbaI 853 TCTAGA T^CTAGA
XcmI 1083 CCAATAAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 259 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 461 GTAGAC GT^[AC][GT]AC
863 GTAGAC
AcyI 89 GGCGCC G[GA]^CG[CT]C
806 GGCGTC
AvaI 34 CCCGGG C^[CT]CG[GA]G
43 CCCGGG
BanII 40 GGGCCC G[GA]GC[CT]^C
1255 GGGCTC
BmyI 40 GGGCCC G[AGT]GC[ACT]^C
1255 GGGCTC
BsaHI 89 GGCGCC G[GA]^CG[CT]C
806 GGCGTC
BsmAI 1061 GAGAC GTCTC(1/5)
1457 GAGAC
BsmFI 640 GGGAC GGGAC(10/14)
616 GTCCC
BsoBI 34 CCCGGG C^[CT]CG[GA]G
43 CCCGGG
Bsp1286I 40 GGGCCC G[AGT]GC[ACT]^C
1255 GGGCTC
Bsp1407I 232 TGTACA T^GTACA
1354 TGTACA
BsrGI 232 TGTACA T^GTACA
1354 TGTACA
BstNI 108 CCTGG CC^[AT]GG
555 CCTGG
EcoRII 108 CCTGG ^CC[AT]GG
555 CCTGG
Hin1I 89 GGCGCC G[GA]^CG[CT]C
806 GGCGTC
HphI 500 GGTGA GGTGA(8/7)
1141 GGTGA
MaeIII 278 GTAAC ^GT[ACGT]AC
1053 GTGAC
MvaI 108 CCTGG CC^[AT]GG
555 CCTGG
NaeI 2 GCCGGC GCC^GGC
92 GCCGGC
NgoMIV 2 GCCGGC G^CCGGC
92 GCCGGC
SfaNI 382 GATGC GCATC(5/9)
1029 GCATC
SmaI 34 CCCGGG CCC^GGG
43 CCCGGG
SspBI 232 TGTACA T^GTACA
1354 TGTACA
TfiI 117 GATTC G^A[AT]TC
1200 GAATC
XmaCI 34 CCCGGG C^CCGGG
43 CCCGGG
XmaI 34 CCCGGG C^CCGGG
43 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI ApoI Asp700I BbvI BcnI
BfaI BglI BsaJI BspHI BspLU11I
BsrFI BsrI BssKI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeI
MboI MboII MnlI MseI MspI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI RcaI RsaI
Sau3AI Sau96I ScrFI SmlI Tru9I
TseI Tsp509I TspRI XmnI