##################################
# NO CUT ENZYMES (  176 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcsI        AcyI        AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BseRI       BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspDI       BspEI       BspHI       BsrBI       
BsrFI       BssHII      BssSI       BstAPI      BstEII      
BstPI       BstUI       BstXI       Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       KasI        KpnI        
KspI        MaeII       MamI        MluI        MluNI       
MroI        MscI        MspA1I      MspI        MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
StuI        SwaI        TfiI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AccI          603  GTATAC               GT^[AC][GT]AC
AfaI         1122  GTAC                 GT^AC
BsgI          935  CTGCAC               GTGCAG(16/14)
Bsp1407I     1121  TGTACA               T^GTACA
BspMI         817  GCAGGT               ACCTGC(4/8)
BsrDI         878  CATTGC               GCAATG(2/0)
BsrGI        1121  TGTACA               T^GTACA
BsrI          891  CCAGT                ACTGG(1/-1)
Bst1107I      603  GTATAC               GTA^TAC
BstBI         395  TTCGAA               TT^CGAA
BstF5I        210  GGATG                GGATG(2/0)
BstYI         354  AGATCC               [GA]^GATC[CT]
BstZ17I       603  GTATAC               GTA^TAC
FokI          210  GGATG                GGATG(9/13)
HinfI         269  GAGTC                G^A[ACGT]TC
MfeI          940  CAATTG               C^AATTG
MflI          354  AGATCC               [GA]^GATC[CT]
MunI          940  CAATTG               C^AATTG
NspV          395  TTCGAA               TT^CGAA
PacI          124  TTAATTAA             TTAAT^TAA
PleI          269  GAGTC                GAGTC(4/5)
PshAI         199  GACTATGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI         1122  GTAC                 GT^AC
SfaNI         319  GATGC                GCATC(5/9)
SfcI          996  CTACAG               C^T[GA][CT]AG
SfuI          395  TTCGAA               TT^CGAA
SspBI        1121  TGTACA               T^GTACA
SspI          842  AATATT               AAT^ATT
XhoII         354  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AflII         850  CTTAAG               C^TTAAG
              896  CTTAAG              
AflIII        866  ACATGT               A^C[GA][CT]GT
             1149  ACATGT              
AlwI          355  GATCC                GGATC(4/5)
              164  GGATC               
AseI          123  ATTAAT               AT^TAAT
              127  ATTAAT              
AsnI          123  ATTAAT               AT^TAAT
              127  ATTAAT              
BfrI          850  CTTAAG               C^TTAAG
              896  CTTAAG              
BsaI          464  GAGACC               GGTCTC(1/5)
               15  GGTCTC              
BspLU11I      866  ACATGT               A^CATGT
             1149  ACATGT              
DsaI          302  CCATGG               C^C[GA][CT]GG
             1091  CCATGG              
EarI         1132  CTCTTC               CTCTTC(1/4)
             1096  GAAGAG              
HaeIII        423  GGCC                 GG^CC
              954  GGCC                
HphI          180  GGTGA                GGTGA(8/7)
              887  TCACC               
Ksp632I      1132  CTCTTC               CTCTTC(1/4)
             1096  GAAGAG              
MslI          511  CATGACTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1144  CATATACATG          
MwoI          520  GCAAAAGCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              775  GCTAGTTGGGC         
NcoI          302  CCATGG               C^CATGG
             1091  CCATGG              
NspI          866  ACATGT               [GA]CATG^[CT]
             1149  ACATGT              
PshBI         123  ATTAAT               AT^TAAT
              127  ATTAAT              
Tsp45I         34  GTCAC                ^GT[GC]AC
              263  GTCAC               
TspRI         366  CACTG                CA[GC]TG[ACGT][ACGT]^
              980  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AluI        BbsI        BbvI        BfaI        BpuAI       
BsaJI       BsmAI       BssKI       BstNI       DdeI        
DpnII       EcoRII      EcoT14I     Fnu4HI      ItaI        
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MvaI        NdeII       NlaIII      Sau3AI      
ScrFI       SmlI        StyI        TaqI        Tru9I       
TseI        Tsp509I     TthHB8I