##################################
# NO CUT ENZYMES (   57 enzymes) #
##################################

AclI        AgeI        AhdI        Alw44I      ApaLI       
AscI        AseI        AsnI        AspEI       AviII       
AvrII       BbrPI       BlnI        BsaAI       BsiEI       
BsiWI       Bsp1407I    BspDI       BsrGI       BssHII      
Bst1107I    BstZ17I     ClaI        CpoI        EagI        
Eam1105I    EclXI       Eco52I      FseI        FspI        
KspI        MfeI        MluI        MunI        NdeI        
NotI        NruI        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RsrII       SacII       SfiI        SgrAI       
SmaI        SnaBI       SspBI       SwaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII        1896  GACGTC               GACGT^C
Acc65I       3167  GGTACC               G^GTACC
AccI         3203  GTCGAC               GT^[AC][GT]AC
AccIII       2510  TCCGGA               T^CCGGA
AflII         244  CTTAAG               C^TTAAG
Asp718I      3167  GGTACC               G^GTACC
AvaI         4142  CTCGGG               C^[CT]CG[GA]G
BcgI         4350  GCATAGCAATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI          244  CTTAAG               C^TTAAG
BglI         6987  GCCAGAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI         2141  GAGACC               GGTCTC(1/5)
BseAI        2510  TCCGGA               T^CCGGA
BsoBI        4142  CTCGGG               C^[CT]CG[GA]G
BspEI        2510  TCCGGA               T^CCGGA
BsrBI        2040  CCGCTC               CCGCTC(-3/-3)
BstAPI       1121  GCATTGTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1031  TTCGAA               TT^CGAA
DraI         7368  TTTAAA               TTT^AAA
DrdI         1798  GACTTGAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV        3634  GATATC               GAT^ATC
EcoT22I      1540  ATGCAT               ATGCA^T
HpaI         4621  GTTAAC               GTT^AAC
KpnI         3167  GGTACC               GGTAC^C
MroI         2510  TCCGGA               T^CCGGA
NaeI         2354  GCCGGC               GCC^GGC
NgoMIV       2354  GCCGGC               G^CCGGC
NsiI         1540  ATGCAT               ATGCA^T
NspV         1031  TTCGAA               TT^CGAA
SalI         3203  GTCGAC               G^TCGAC
SapI         3984  GCTCTTC              GCTCTTC(1/4)
SbfI         2370  CCTGCAGG             CCTGCA^GG
SfuI         1031  TTCGAA               TT^CGAA
SpeI         4597  ACTAGT               A^CTAGT
SphI         4967  GCATGC               GCATG^C
Sse8387I     2370  CCTGCAGG             CCTGCA^GG
SspI         3342  AATATT               AAT^ATT
StuI         3964  AGGCCT               AGG^CCT
XbaI         2586  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AflIII       2099  ACATGT               A^C[GA][CT]GT
             4676  ACATGT              
Aor51HI       409  AGCGCT               AGC^GCT
             1929  AGCGCT              
AspI          904  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5395  GACATTGTC           
BciVI         519  GGATAC               GTATCC(6/5)
             5325  GGATAC              
BsaBI        1012  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4390  GATGGGGATC          
BsmBI        2929  CGTCTC               CGTCTC(1/5)
             1894  GAGACG              
BsmI         3113  GAATGC               GAATGC(1/-1)
             4275  GAATGC              
BspLU11I     2099  ACATGT               A^CATGT
             4676  ACATGT              
Eco47III      409  AGCGCT               AGC^GCT
             1929  AGCGCT              
EcoRI        1225  GAATTC               G^AATTC
             3896  GAATTC              
HindIII      1035  AAGCTT               A^AGCTT
             5644  AAGCTT              
MamI         1012  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4390  GATGGGGATC          
NheI         1248  GCTAGC               G^CTAGC
             6027  GCTAGC              
PflFI         904  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5395  GACATTGTC           
PpuMI        7034  AGGACCC              [GA]G^G[AT]CC[CT]
             7318  GGGTCCC             
ScaI         4973  AGTACT               AGT^ACT
             5988  AGTACT              
Tth111I       904  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5395  GACATTGTC           
XcmI         3445  CCACCTTTGCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             5675  CCAACCTGCAGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  145 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       AocI        ApaI        
ApoI        Asp700I     AspHI       AvaII       BalI        
BamHI       BanI        BanII       BbeI        BbsI        
BbvCI       BbvI        BclI        BcnI        BfaI        
BglII       BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaHI       BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspHI       BspMI       BsrDI       
BsrFI       BsrI        BssKI       BssSI       BstEII      
BstF5I      BstNI       BstPI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DraIII      DsaI        EaeI        EarI        Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRII      EcoT14I     
FauI        FbaI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NarI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PshAI       
PstI        PvuII       RcaI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SexAI       SfaNI       
SfcI        SfoI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII       XmnI