##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AflII       
AflIII      AgeI        AhdI        Aor51HI     ApaI        
AscI        Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BanI        BbeI        BbrPI       
BcgI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiWI       BsoBI       
BspEI       BspHI       BspLU11I    BsrBI       BssHII      
BssSI       BstEII      BstPI       CelII       CpoI        
DrdI        Eam1105I    Eco47III    EcoNI       EcoO65I     
EcoRI       FseI        FspI        Hin1I       HpaI        
KasI        KpnI        KspI        MamI        MluI        
MroI        NarI        NheI        NotI        NruI        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    StuI        SwaI        
Tth111I     XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   77 enzymes) #
###################################

AccII          42  CGCG                 CG^CG
AclI         2157  AACGTT               AA^CGTT
Alw44I       1155  GTGCAC               G^TGCAC
AlwNI        1116  CAGACACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          360  CCTTAGG              CC^T[ACGT]AGG
ApaLI        1155  GTGCAC               G^TGCAC
AseI          242  ATTAAT               AT^TAAT
AsnI          242  ATTAAT               AT^TAAT
Asp700I      2096  GAAAGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         715  GGACC                G^G[AT]CC
BalI         1043  TGGCCA               TGG^CCA
BamHI        1696  GGATCC               G^GATCC
BanII         118  GGGCTC               G[GA]GC[CT]^C
BbvCI        2288  CCTCAGC              CCTCAGC(-5/-2)
BciVI        2339  GTATCC               GTATCC(6/5)
BclI          352  TGATCA               T^GATCA
BcnI         1140  CCCGG                CC^[GC]GG
BglI          307  GCCAACAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI         1407  CTGCAC               GTGCAG(16/14)
BsiEI          30  CGGCCG               CG[GA][CT]^CG
BsmI         2610  GAATGC               GAATGC(1/-1)
Bsp1407I      532  TGTACA               T^GTACA
BspDI         267  ATCGAT               AT^CGAT
BspMI         260  GCAGGT               ACCTGC(4/8)
BsrFI         485  GCCGGC               [GA]^CCGG[CT]
BsrGI         532  TGTACA               T^GTACA
BstAPI        542  GCATGTATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         888  TTCGAA               TT^CGAA
BstUI          42  CGCG                 CG^CG
BstYI        1696  GGATCC               [GA]^GATC[CT]
Bsu36I        360  CCTTAGG              CC^T[ACGT]AGG
CfoI            2  GCGC                 GCG^C
Cfr10I        485  GCCGGC               [GA]^CCGG[CT]
ClaI          267  ATCGAT               AT^CGAT
DraIII       1635  CACTATGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI           30  CGGCCG               C^GGCCG
EclXI          30  CGGCCG               C^GGCCG
Eco52I         30  CGGCCG               C^GGCCG
Eco81I        360  CCTTAGG              CC^T[ACGT]AGG
EcoRV         683  GATATC               GAT^ATC
EcoT22I      1229  ATGCAT               ATGCA^T
FbaI          352  TGATCA               T^GATCA
HaeII           1  GGCGCT               [GA]GCGC^[CT]
HgaI           40  GACGC                GACGC(5/10)
HhaI            2  GCGC                 GCG^C
HinP1I          2  GCGC                 G^CGC
HincII       2538  GTTGAC               GT[CT]^[GA]AC
HindII       2538  GTTGAC               GT[CT]^[GA]AC
HindIII       917  AAGCTT               A^AGCTT
MfeI          999  CAATTG               C^AATTG
MflI         1696  GGATCC               [GA]^GATC[CT]
MluNI        1043  TGGCCA               TGG^CCA
MscI         1043  TGGCCA               TGG^CCA
MunI          999  CAATTG               C^AATTG
MvnI           42  CGCG                 CG^CG
NaeI          485  GCCGGC               GCC^GGC
NciI         1140  CCCGG                CC^[GC]GG
NdeI          780  CATATG               CA^TATG
NgoMIV        485  GCCGGC               G^CCGGC
NsiI         1229  ATGCAT               ATGCA^T
NspV          888  TTCGAA               TT^CGAA
PflMI        1915  CCAAGTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         1822  GACTC                GAGTC(4/5)
PpuMI         714  GGGACCT              [GA]G^G[AT]CC[CT]
PshBI         242  ATTAAT               AT^TAAT
Psp1406I     2157  AACGTT               AA^CGTT
PstI           64  CTGCAG               CTGCA^G
SapI            4  GCTCTTC              GCTCTTC(1/4)
SexAI         499  ACCAGGT              A^CC[AT]GGT
SfuI          888  TTCGAA               TT^CGAA
SmlI         1331  CTCAAG               C^T[CT][GA]AG
SphI          859  GCATGC               GCATG^C
SspBI         532  TGTACA               T^GTACA
SspI         2596  AATATT               AAT^ATT
Van91I       1915  CCAAGTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII        1696  GGATCC               [GA]^GATC[CT]
XmnI         2096  GAAAGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI         1599  GTATAC               GT^[AC][GT]AC
             1617  GTATAC              
AlwI         1697  GATCC                GGATC(4/5)
             1696  GGATC               
BbsI         2348  GAAGAC               GAAGAC(2/6)
              217  GTCTTC              
BfaI          462  CTAG                 C^TAG
             1888  CTAG                
BpuAI        2348  GAAGAC               GAAGAC(2/6)
              217  GTCTTC              
BsmBI          38  GAGACG               CGTCTC(1/5)
             1071  GAGACG              
BsmFI         714  GGGAC                GGGAC(10/14)
               90  GTCCC               
BsrDI        1379  CATTGC               GCAATG(2/0)
              699  GCAATG              
Bst1107I     1599  GTATAC               GTA^TAC
             1617  GTATAC              
BstXI         865  CCAAGCAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1046  CCAGATGGTTGG        
BstZ17I      1599  GTATAC               GTA^TAC
             1617  GTATAC              
DraII         650  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              714  GGGACCT             
EaeI           30  CGGCCG               [CT]^GGCC[GA]
             1043  TGGCCA              
EcoO109I      650  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              714  GGGACCT             
FauI           13  GCGGG                CCCGC(4/6)
               43  GCGGG               
HapII         486  CCGG                 C^CGG
             1141  CCGG                
HpaII         486  CCGG                 C^CGG
             1141  CCGG                
MaeI          462  CTAG                 C^TAG
             1888  CTAG                
MaeII        2158  ACGT                 A^CGT
             2307  ACGT                
MspI          486  CCGG                 C^CGG
             1141  CCGG                
NcoI          254  CCATGG               C^CATGG
              631  CCATGG              
NspI          542  GCATGT               [GA]CATG^[CT]
              859  GCATGC              
TaqI          268  TCGA                 T^CGA
              889  TCGA                
TthHB8I       268  TCGA                 T^CGA
              889  TCGA                
XcmI         1511  CCAGGTGAACATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1908  CCATTTTCCAAGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AspHI       BbvI        BmyI        BpmI        BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DraI        DsaI        
EarI        EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        Ksp632I     
MaeIII      MboI        MboII       MnlI        MseI        
MslI        MspA1I      MvaI        MwoI        NdeII       
NlaIII      NlaIV       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI