##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI AhdI Alw44I ApaI ApaLI
AscI Asp718I AspEI AspI AviII
BamHI BbeI BbrPI BcgI BclI
BfrI BglI BlpI Bpu1102I BsaHI
BseAI BsgI BsiEI BsiWI BsmBI
BspDI BspEI BsrBI BssHII Bst1107I
BstZ17I CelII ClaI CpoI DrdI
DsaI EagI Eam1105I EclXI Eco52I
FbaI FseI FspI Hin1I HpaI
KasI KpnI KspI MluI MroI
NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PvuI
RsrII SacII SalI SapI SbfI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 3308 GTAGAC GT^[AC][GT]AC
AclI 4022 AACGTT AA^CGTT
Aor51HI 4047 AGCGCT AGC^GCT
AseI 4567 ATTAAT AT^TAAT
AsnI 4567 ATTAAT AT^TAAT
AvaI 2574 CTCGGG C^[CT]CG[GA]G
AvrII 388 CCTAGG C^CTAGG
BalI 2874 TGGCCA TGG^CCA
BciVI 4957 GGATAC GTATCC(6/5)
BcnI 2855 CCGGG CC^[GC]GG
BglII 2892 AGATCT A^GATCT
BlnI 388 CCTAGG C^CTAGG
BsaBI 4477 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 2574 CTCGGG C^[CT]CG[GA]G
BspHI 2095 TCATGA T^CATGA
BsrDI 3500 GCAATG GCAATG(2/0)
BsrFI 336 ACCGGC [GA]^CCGG[CT]
BssSI 5300 CTCGTG CACGAG(-5/-1)
BstAPI 800 GCACAACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3421 TTCGAA TT^CGAA
BstEII 1315 GGTGACC G^GT[ACGT]ACC
BstPI 1315 GGTGACC G^GT[ACGT]ACC
BstXI 1133 CCACCAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 336 ACCGGC [GA]^CCGG[CT]
EaeI 2874 TGGCCA [CT]^GGCC[GA]
Eco47III 4047 AGCGCT AGC^GCT
EcoNI 1632 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1315 GGTGACC G^GT[ACGT]ACC
HaeII 4047 AGCGCT [GA]GCGC^[CT]
HgaI 55 GCGTC GACGC(5/10)
HindIII 5165 AAGCTT A^AGCTT
MamI 4477 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2748 CAATTG C^AATTG
MluNI 2874 TGGCCA TGG^CCA
MscI 2874 TGGCCA TGG^CCA
MunI 2748 CAATTG C^AATTG
NciI 2855 CCGGG CC^[GC]GG
NdeI 5420 CATATG CA^TATG
NspV 3421 TTCGAA TT^CGAA
PpuMI 698 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 2619 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4567 ATTAAT AT^TAAT
Psp1406I 4022 AACGTT AA^CGTT
RcaI 2095 TCATGA T^CATGA
SfuI 3421 TTCGAA TT^CGAA
SphI 1372 GCATGC GCATG^C
XcmI 2761 CCAGGGCTTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 54 CGCG CG^CG
94 CGCG
AlwNI 615 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
768 CAGATGCTG
AocI 752 CCTCAGG CC^T[ACGT]AGG
2173 CCTCAGG
BbvCI 326 CCTCAGC CCTCAGC(-5/-2)
575 GCTGAGG
BsaAI 209 TACGTG [CT]AC^GT[GA]
3184 TACGTG
BsaWI 58 TCCGGT [AT]^CCGG[AT]
1752 ACCGGA
BsmFI 5381 GGGAC GGGAC(10/14)
5256 GTCCC
BstUI 54 CGCG CG^CG
94 CGCG
Bsu36I 752 CCTCAGG CC^T[ACGT]AGG
2173 CCTCAGG
DraIII 801 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
4393 CACCCTGTG
Eco81I 752 CCTCAGG CC^T[ACGT]AGG
2173 CCTCAGG
EcoRI 3596 GAATTC G^AATTC
3939 GAATTC
EcoT14I 388 CCTAGG C^C[AT][AT]GG
691 CCTTGG
HincII 851 GTTGAC GT[CT]^[GA]AC
4599 GTCAAC
HindII 851 GTTGAC GT[CT]^[GA]AC
4599 GTCAAC
MspA1I 1247 CAGCTG C[AC]G^C[GT]G
5261 CAGCTG
MvnI 54 CGCG CG^CG
94 CGCG
PstI 1269 CTGCAG CTGCA^G
5264 CTGCAG
PvuII 1247 CAGCTG CAG^CTG
5261 CAGCTG
SacI 1036 GAGCTC GAGCT^C
2670 GAGCTC
ScaI 4460 AGTACT AGT^ACT
4787 AGTACT
SexAI 1135 ACCAGGT A^CC[AT]GGT
4623 ACCAGGT
SmlI 1255 CTCAAG C^T[CT][GA]AG
4106 CTCAAG
StyI 388 CCTAGG C^C[AT][AT]GG
691 CCTTGG
SwaI 5120 ATTTAAAT ATTT^AAAT
5521 ATTTAAAT
XbaI 722 TCTAGA T^CTAGA
5428 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaII
BanI BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmI Bsp1286I Bsp1407I BspLU11I BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRII EcoRV EcoT22I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI SspI StuI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI