##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AgeI        AhdI        AlwNI       AocI        
Aor51HI     ApaI        AscI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BcnI        BglI        BglII       BlnI        
BpuAI       BsaAI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BspDI       BspEI       BsrBI       
BsrFI       BssHII      Bst1107I    BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CfoI        
Cfr10I      ClaI        CpoI        DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRV       EcoT14I     
FauI        FseI        FspI        HaeII       HapII       
HhaI        HinP1I      HpaII       KasI        KpnI        
KspI        MaeII       MluI        MluNI       MroI        
MscI        MspI        MvnI        NaeI        NarI        
NciI        NcoI        NgoMIV      NheI        NotI        
NruI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SfiI        SfoI        SgrAI       SmaI        SnaBI       
Sse8387I    StuI        StyI        Tth111I     XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AcyI           62  GGCGTC               G[GA]^CG[CT]C
Alw44I       2044  GTGCAC               G^TGCAC
ApaLI        2044  GTGCAC               G^TGCAC
AvaI         1687  CCCGAG               C^[CT]CG[GA]G
BamHI        2659  GGATCC               G^GATCC
BanI         1900  GGTGCC               G^G[CT][GA]CC
BlpI          108  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      108  GCTGAGC              GC^T[ACGT]AGC
BsaBI        2371  GATATAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          62  GGCGTC               G[GA]^CG[CT]C
BsgI         1738  CTGCAC               GTGCAG(16/14)
BsmFI        1347  GGGAC                GGGAC(10/14)
BsmI          143  GCATTC               GAATGC(1/-1)
BsoBI        1687  CCCGAG               C^[CT]CG[GA]G
BspMI        1587  GCAGGT               ACCTGC(4/8)
BsrDI         940  GCAATG               GCAATG(2/0)
BssSI         988  CACGAG               CACGAG(-5/-1)
BstBI        1228  TTCGAA               TT^CGAA
CelII         108  GCTGAGC              GC^T[ACGT]AGC
DraIII        988  CACGAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          880  CCGTGG               C^C[GA][CT]GG
EarI         2547  GAAGAG               CTCTTC(1/4)
EcoT22I      2511  ATGCAT               ATGCA^T
HaeIII        662  GGCC                 GG^CC
Hin1I          62  GGCGTC               G[GA]^CG[CT]C
HindIII       931  AAGCTT               A^AGCTT
HpaI         2405  GTTAAC               GTT^AAC
Ksp632I      2547  GAAGAG               CTCTTC(1/4)
MamI         2371  GATATAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI          157  CATATG               CA^TATG
NsiI         2511  ATGCAT               ATGCA^T
NspV         1228  TTCGAA               TT^CGAA
PacI         1577  TTAATTAA             TTAAT^TAA
PflMI         875  CCATGCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1598  AGGACCC              [GA]G^G[AT]CC[CT]
PshAI         536  GACATCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          457  CTGCAG               CTGCA^G
SexAI        1485  ACCAGGT              A^CC[AT]GGT
SfaNI         908  GCATC                GCATC(5/9)
SfuI         1228  TTCGAA               TT^CGAA
SpeI         2558  ACTAGT               A^CTAGT
SwaI         1657  ATTTAAAT             ATTT^AAAT
Van91I        875  CCATGCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AflII         270  CTTAAG               C^TTAAG
             2664  CTTAAG              
AflIII        593  ACATGT               A^C[GA][CT]GT
             2401  ACATGT              
AspHI        2044  GTGCAC               G[AT]GC[AT]^C
             2803  GTGCTC              
AvaII        1348  GGACC                G^G[AT]CC
             1599  GGACC               
BanII        2598  GAGCCC               G[GA]GC[CT]^C
             2772  GAGCCC              
BfrI          270  CTTAAG               C^TTAAG
             2664  CTTAAG              
BpmI         1248  CTGGAG               CTGGAG(16/14)
             1384  CTGGAG              
BseRI        1028  CTCCTC               GAGGAG(10/8)
             1047  GAGGAG              
BsiHKAI      2044  GTGCAC               G[AT]GC[AT]^C
             2803  GTGCTC              
Bsp1407I     2325  TGTACA               T^GTACA
             2350  TGTACA              
BspHI        2249  TCATGA               T^CATGA
             2621  TCATGA              
BspLU11I      593  ACATGT               A^CATGT
             2401  ACATGT              
BsrGI        2325  TGTACA               T^GTACA
             2350  TGTACA              
BstAPI       1854  GCAGTGCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1894  GCATGAGGTGC         
BstYI         620  GGATCT               [GA]^GATC[CT]
             2659  GGATCC              
DraII         661  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1598  AGGACCC             
EcoO109I      661  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1598  AGGACCC             
EcoRI         401  GAATTC               G^AATTC
             1439  GAATTC              
HincII       2405  GTTAAC               GT[CT]^[GA]AC
             2831  GTCAAC              
HindII       2405  GTTAAC               GT[CT]^[GA]AC
             2831  GTCAAC              
HphI         2810  GGTGA                GGTGA(8/7)
             1106  TCACC               
MfeI          355  CAATTG               C^AATTG
              489  CAATTG              
MflI          620  GGATCT               [GA]^GATC[CT]
             2659  GGATCC              
MslI         1656  CATTTAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2574  CACACTCATG          
MspA1I        745  CAGCTG               C[AC]G^C[GT]G
             1245  CAGCTG              
MunI          355  CAATTG               C^AATTG
              489  CAATTG              
PvuII         745  CAGCTG               CAG^CTG
             1245  CAGCTG              
RcaI         2249  TCATGA               T^CATGA
             2621  TCATGA              
ScaI          371  AGTACT               AGT^ACT
             2561  AGTACT              
SphI          518  GCATGC               GCATG^C
              730  GCATGC              
SspBI        2325  TGTACA               T^GTACA
             2350  TGTACA              
SspI         2307  AATATT               AAT^ATT
             2607  AATATT              
XbaI          345  TCTAGA               T^CTAGA
              764  TCTAGA              
XhoII         620  GGATCT               [GA]^GATC[CT]
             2659  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AseI        AsnI        BbvI        BclI        
BfaI        BmyI        BsaI        BsaJI       BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       DraI        EcoRII      FbaI        Fnu4HI      
FokI        HgaI        HinfI       ItaI        MaeI        
MaeIII      MboI        MboII       MnlI        MseI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PleI        PshBI       RsaI        Sau3AI      
Sau96I      ScrFI       SfcI        SmlI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I