##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AvrII BfrI
BlnI BsaBI BsiWI Bsp1407I BspDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraIII EagI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FseI
HpaI KpnI MamI MluI NdeI
NheI NotI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I RsrII SalI SbfI SexAI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 740 GTAGAC GT^[AC][GT]AC
AccIII 2625 TCCGGA T^CCGGA
AhdI 1969 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1969 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3906 TGCGCA TGC^GCA
BbrPI 2542 CACGTG CAC^GTG
BbsI 3947 GTCTTC GAAGAC(2/6)
BcgI 94 CGAATGGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1963 AGATCT A^GATCT
BlpI 1156 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1156 GCTGAGC GC^T[ACGT]AGC
BpuAI 3947 GTCTTC GAAGAC(2/6)
BsaAI 2542 CACGTG [CT]AC^GT[GA]
BsaI 3246 GAGACC GGTCTC(1/5)
BseAI 2625 TCCGGA T^CCGGA
BsmBI 3314 GAGACG CGTCTC(1/5)
BspEI 2625 TCCGGA T^CCGGA
BsrBI 3433 GAGCGG CCGCTC(-3/-3)
BssSI 527 CTCGTG CACGAG(-5/-1)
BstAPI 3610 GCAGCTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1156 GCTGAGC GC^T[ACGT]AGC
DrdI 240 GACTCAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1969 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3364 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 632 GAATTC G^AATTC
FspI 3906 TGCGCA TGC^GCA
KspI 119 CCGCGG CCGC^GG
MroI 2625 TCCGGA T^CCGGA
PmaCI 2542 CACGTG CAC^GTG
PmlI 2542 CACGTG CAC^GTG
PpuMI 2523 AGGTCCT [GA]G^G[AT]CC[CT]
PvuI 3392 CGATCG CGAT^CG
SacI 155 GAGCTC GAGCT^C
SacII 119 CCGCGG CCGC^GG
ScaI 2598 AGTACT AGT^ACT
SfiI 42 GGCCTCGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1018 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Asp700I 1029 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4300 GAAAATTTTC
BalI 2181 TGGCCA TGG^CCA
2835 TGGCCA
BamHI 3747 GGATCC G^GATCC
3846 GGATCC
BciVI 680 GGATAC GTATCC(6/5)
2740 GGATAC
BsaWI 2625 TCCGGA [AT]^CCGG[AT]
2744 ACCGGA
BsgI 2289 GTGCAG GTGCAG(16/14)
2866 GTGCAG
BspHI 2244 TCATGA T^CATGA
4132 TCATGA
HindIII 500 AAGCTT A^AGCTT
4333 AAGCTT
MfeI 745 CAATTG C^AATTG
3037 CAATTG
MluNI 2181 TGGCCA TGG^CCA
2835 TGGCCA
MscI 2181 TGGCCA TGG^CCA
2835 TGGCCA
MunI 745 CAATTG C^AATTG
3037 CAATTG
NaeI 146 GCCGGC GCC^GGC
3895 GCCGGC
NgoMIV 146 GCCGGC G^CCGGC
3895 GCCGGC
NruI 68 TCGCGA TCG^CGA
2874 TCGCGA
RcaI 2244 TCATGA T^CATGA
4132 TCATGA
SapI 727 GAAGAGC GCTCTTC(1/4)
984 GAAGAGC
SmaI 137 CCCGGG CCC^GGG
1856 CCCGGG
SmlI 902 CTCAAG C^T[CT][GA]AG
3405 CTCAAG
XmaCI 137 CCCGGG C^CCGGG
1856 CCCGGG
XmaI 137 CCCGGG C^CCGGG
1856 CCCGGG
XmnI 1029 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4300 GAAAATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AspI AvaI AvaII
BanI BanII BbeI BbvCI BbvI
BclI BcnI BfaI BglI BmyI
BpmI BsaHI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspLU11I BspMI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII