##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AvrII       BfrI        
BlnI        BsaBI       BsiWI       Bsp1407I    BspDI       
BsrGI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      ClaI        CpoI        
DraIII      EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRV       EcoT22I     FseI        
HpaI        KpnI        MamI        MluI        NdeI        
NheI        NotI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshAI       PshBI       
Psp1406I    RsrII       SalI        SbfI        SexAI       
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI          740  GTAGAC               GT^[AC][GT]AC
AccIII       2625  TCCGGA               T^CCGGA
AhdI         1969  GACTGAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        1969  GACTGAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        3906  TGCGCA               TGC^GCA
BbrPI        2542  CACGTG               CAC^GTG
BbsI         3947  GTCTTC               GAAGAC(2/6)
BcgI           94  CGAATGGAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        1963  AGATCT               A^GATCT
BlpI         1156  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1156  GCTGAGC              GC^T[ACGT]AGC
BpuAI        3947  GTCTTC               GAAGAC(2/6)
BsaAI        2542  CACGTG               [CT]AC^GT[GA]
BsaI         3246  GAGACC               GGTCTC(1/5)
BseAI        2625  TCCGGA               T^CCGGA
BsmBI        3314  GAGACG               CGTCTC(1/5)
BspEI        2625  TCCGGA               T^CCGGA
BsrBI        3433  GAGCGG               CCGCTC(-3/-3)
BssSI         527  CTCGTG               CACGAG(-5/-1)
BstAPI       3610  GCAGCTCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        1156  GCTGAGC              GC^T[ACGT]AGC
DrdI          240  GACTCAGTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1969  GACTGAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        3364  CCTGGACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         632  GAATTC               G^AATTC
FspI         3906  TGCGCA               TGC^GCA
KspI          119  CCGCGG               CCGC^GG
MroI         2625  TCCGGA               T^CCGGA
PmaCI        2542  CACGTG               CAC^GTG
PmlI         2542  CACGTG               CAC^GTG
PpuMI        2523  AGGTCCT              [GA]G^G[AT]CC[CT]
PvuI         3392  CGATCG               CGAT^CG
SacI          155  GAGCTC               GAGCT^C
SacII         119  CCGCGG               CCGC^GG
ScaI         2598  AGTACT               AGT^ACT
SfiI           42  GGCCTCGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI         1018  GCATGC               GCATG^C


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

Asp700I      1029  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4300  GAAAATTTTC          
BalI         2181  TGGCCA               TGG^CCA
             2835  TGGCCA              
BamHI        3747  GGATCC               G^GATCC
             3846  GGATCC              
BciVI         680  GGATAC               GTATCC(6/5)
             2740  GGATAC              
BsaWI        2625  TCCGGA               [AT]^CCGG[AT]
             2744  ACCGGA              
BsgI         2289  GTGCAG               GTGCAG(16/14)
             2866  GTGCAG              
BspHI        2244  TCATGA               T^CATGA
             4132  TCATGA              
HindIII       500  AAGCTT               A^AGCTT
             4333  AAGCTT              
MfeI          745  CAATTG               C^AATTG
             3037  CAATTG              
MluNI        2181  TGGCCA               TGG^CCA
             2835  TGGCCA              
MscI         2181  TGGCCA               TGG^CCA
             2835  TGGCCA              
MunI          745  CAATTG               C^AATTG
             3037  CAATTG              
NaeI          146  GCCGGC               GCC^GGC
             3895  GCCGGC              
NgoMIV        146  GCCGGC               G^CCGGC
             3895  GCCGGC              
NruI           68  TCGCGA               TCG^CGA
             2874  TCGCGA              
RcaI         2244  TCATGA               T^CATGA
             4132  TCATGA              
SapI          727  GAAGAGC              GCTCTTC(1/4)
              984  GAAGAGC             
SmaI          137  CCCGGG               CCC^GGG
             1856  CCCGGG              
SmlI          902  CTCAAG               C^T[CT][GA]AG
             3405  CTCAAG              
XmaCI         137  CCCGGG               C^CCGGG
             1856  CCCGGG              
XmaI          137  CCCGGG               C^CCGGG
             1856  CCCGGG              
XmnI         1029  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4300  GAAAATTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  128 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       ApaI        
ApoI        AspHI       AspI        AvaI        AvaII       
BanI        BanII       BbeI        BbvCI       BbvI        
BclI        BcnI        BfaI        BglI        BmyI        
BpmI        BsaHI       BsaJI       BseRI       BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    BspLU11I    BspMI       
BsrDI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        FbaI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
KasI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PflFI       PflMI       
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Tth111I     TthHB8I     
Van91I      XcmI        XhoII