##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        Aor51HI     AscI        Asp718I     AspI        
AviII       AvrII       BbrPI       BcgI        BciVI       
BfrI        BlnI        BsaBI       BsaWI       BseAI       
BsiWI       BspDI       BspEI       BspHI       BsrFI       
BssHII      Bst1107I    BstEII      BstPI       BstZ17I     
Cfr10I      ClaI        CpoI        DraIII      DrdI        
Eco47III    EcoO65I     EcoT22I     FseI        FspI        
HpaI        KpnI        MamI        MfeI        MluI        
MroI        MunI        NaeI        NgoMIV      NheI        
NruI        NsiI        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PvuI        RcaI        RsrII       SalI        SapI        
SexAI       SfiI        SgrAI       SnaBI       SpeI        
SphI        SspI        Tth111I     


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

Alw44I       1752  GTGCAC               G^TGCAC
ApaLI        1752  GTGCAC               G^TGCAC
BbeI          221  GGCGCC               GGCGC^C
BbvCI        3496  GCTGAGG              CCTCAGC(-5/-2)
BglII         212  AGATCT               A^GATCT
BsaI         4934  GGTCTC               GGTCTC(1/5)
BsiEI          53  CGGCCG               CG[GA][CT]^CG
BspLU11I     3883  ACATGT               A^CATGT
BsrDI        3687  CATTGC               GCAATG(2/0)
BssSI        1129  CTCGTG               CACGAG(-5/-1)
BstAPI       1866  GCAGTCCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        2051  TTCGAA               TT^CGAA
EagI           53  CGGCCG               C^GGCCG
EclXI          53  CGGCCG               C^GGCCG
Eco52I         53  CGGCCG               C^GGCCG
EcoNI        2610  CCTTCCTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        2042  GATATC               GAT^ATC
FauI         2633  CCCGC                CCCGC(4/6)
HincII        229  GTTGAC               GT[CT]^[GA]AC
HindII        229  GTTGAC               GT[CT]^[GA]AC
KasI          221  GGCGCC               G^GCGCC
KspI           56  CCGCGG               CCGC^GG
NarI          221  GGCGCC               GG^CGCC
NotI           52  GCGGCCGC             GC^GGCCGC
NspV         2051  TTCGAA               TT^CGAA
PaeR7I       2828  CTCGAG               C^TCGAG
SacII          56  CCGCGG               CCGC^GG
SbfI          402  CCTGCAGG             CCTGCA^GG
ScaI         2375  AGTACT               AGT^ACT
SfoI          221  GGCGCC               GGC^GCC
SfuI         2051  TTCGAA               TT^CGAA
Sse8387I      402  CCTGCAGG             CCTGCA^GG
StuI         3324  AGGCCT               AGG^CCT
SwaI         5057  ATTTAAAT             ATTT^AAAT
XbaI         3373  TCTAGA               T^CTAGA
XcmI         2522  CCATCAGTGCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         2828  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

AccII          57  CGCG                 CG^CG
             2241  CGCG                
AflIII       2879  ACGTGT               A^C[GA][CT]GT
             3883  ACATGT              
AhdI         1771  GACAAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4449  GACTGAATGTC         
AlwNI         658  CAGCACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             4659  CAGAATCTG           
AocI         1616  CCTCAGG              CC^T[ACGT]AGG
             2614  CCTAAGG             
AseI         4424  ATTAAT               AT^TAAT
             5246  ATTAAT              
AsnI         4424  ATTAAT               AT^TAAT
             5246  ATTAAT              
Asp700I      1784  GAATGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3377  GAAATCTTTC          
AspEI        1771  GACAAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4449  GACTGAATGTC         
BclI          287  TGATCA               T^GATCA
             2924  TGATCA              
BglI         1197  GCCTGAGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2530  GCCATGGAGGC         
BlpI         3081  GCTCAGC              GC^T[ACGT]AGC
             4876  GCTCAGC             
Bpu1102I     3081  GCTCAGC              GC^T[ACGT]AGC
             4876  GCTCAGC             
BsaAI        2878  TACGTG               [CT]AC^GT[GA]
             5281  CACGTA              
Bsp1407I     2697  TGTACA               T^GTACA
             4118  TGTACA              
BsrBI        1185  GAGCGG               CCGCTC(-3/-3)
             1201  GAGCGG              
BsrGI        2697  TGTACA               T^GTACA
             4118  TGTACA              
BstUI          57  CGCG                 CG^CG
             2241  CGCG                
Bsu36I       1616  CCTCAGG              CC^T[ACGT]AGG
             2614  CCTAAGG             
CelII        3081  GCTCAGC              GC^T[ACGT]AGC
             4876  GCTCAGC             
Eam1105I     1771  GACAAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4449  GACTGAATGTC         
Eco81I       1616  CCTCAGG              CC^T[ACGT]AGG
             2614  CCTAAGG             
FbaI          287  TGATCA               T^GATCA
             2924  TGATCA              
HaeII         221  GGCGCC               [GA]GCGC^[CT]
             1853  AGCGCC              
HindIII      1624  AAGCTT               A^AGCTT
             3947  AAGCTT              
MslI         4160  CACTCGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             4604  CATACATATG          
MvnI           57  CGCG                 CG^CG
             2241  CGCG                
NcoI         2531  CCATGG               C^CATGG
             5270  CCATGG              
NdeI         4370  CATATG               CA^TATG
             4608  CATATG              
NspI         3883  ACATGT               [GA]CATG^[CT]
             4862  GCATGT              
PpuMI         821  GGGTCCT              [GA]G^G[AT]CC[CT]
             2489  AGGTCCT             
PshBI        4424  ATTAAT               AT^TAAT
             5246  ATTAAT              
SacI          717  GAGCTC               GAGCT^C
             2419  GAGCTC              
SmaI          818  CCCGGG               CCC^GGG
              857  CCCGGG              
SspBI        2697  TGTACA               T^GTACA
             4118  TGTACA              
XmaCI         818  CCCGGG               C^CCGGG
              857  CCCGGG              
XmaI          818  CCCGGG               C^CCGGG
              857  CCCGGG              
XmnI         1784  GAATGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3377  GAAATCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AccI        AciI        AcsI        AcyI        AfaI        
AluI        AlwI        ApaI        ApoI        AspHI       
AvaI        AvaII       BalI        BamHI       BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaHI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRI       
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII