##################################
# NO CUT ENZYMES (   62 enzymes) #
##################################

AatII       AclI        AcyI        AhdI        Aor51HI     
ApaI        AscI        AspEI       AviII       BbeI        
BbrPI       BcgI        BglI        BsaAI       BsaHI       
BsiEI       BsiWI       BsmBI       BspDI       BsrBI       
BssHII      BstAPI      BstEII      BstPI       ClaI        
CpoI        DraIII      EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     FseI        FspI        
HgaI        Hin1I       KasI        KspI        MluI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        PacI        PmaCI       PmeI        PmlI        
Psp1406I    PvuI        RsrII       SacII       SbfI        
SexAI       SfiI        SfoI        SgrAI       SnaBI       
Sse8387I    SwaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

Acc65I       5453  GGTACC               G^GTACC
AccII        1760  CGCG                 CG^CG
AccIII       5588  TCCGGA               T^CCGGA
AflIII       1752  ACATGT               A^C[GA][CT]GT
AgeI         1100  ACCGGT               A^CCGGT
Asp718I      5453  GGTACC               G^GTACC
AspI         5637  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII         343  CCTAGG               C^CTAGG
BbvCI        5249  CCTCAGC              CCTCAGC(-5/-2)
BlnI          343  CCTAGG               C^CTAGG
BseAI        5588  TCCGGA               T^CCGGA
BseRI        5216  CTCCTC               GAGGAG(10/8)
BspEI        5588  TCCGGA               T^CCGGA
BspLU11I     1752  ACATGT               A^CATGT
BsrFI        1100  ACCGGT               [GA]^CCGG[CT]
BssSI         392  CTCGTG               CACGAG(-5/-1)
Bst1107I     2419  GTATAC               GTA^TAC
BstBI        3465  TTCGAA               TT^CGAA
BstUI        1760  CGCG                 CG^CG
BstXI        2251  CCAGCATTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      2419  GTATAC               GTA^TAC
Cfr10I       1100  ACCGGT               [GA]^CCGG[CT]
DrdI         3885  GACGTTTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1681  CCTTCAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII         906  GGCGCT               [GA]GCGC^[CT]
HpaI         1000  GTTAAC               GTT^AAC
KpnI         5453  GGTACC               GGTAC^C
MroI         5588  TCCGGA               T^CCGGA
MvnI         1760  CGCG                 CG^CG
NspV         3465  TTCGAA               TT^CGAA
PflFI        5637  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1100  ACCGGT               A^CCGGT
PshAI        5695  GACCAGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI         4254  GTCGAC               G^TCGAC
SfuI         3465  TTCGAA               TT^CGAA
SphI         4831  GCATGC               GCATG^C
Tth111I      5637  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   50 enzymes) #
###################################

AflII        2899  CTTAAG               C^TTAAG
             4990  CTTAAG              
AocI         3283  CCTAAGG              CC^T[ACGT]AGG
             5448  CCTCAGG             
AseI          577  ATTAAT               AT^TAAT
             4747  ATTAAT              
AsnI          577  ATTAAT               AT^TAAT
             4747  ATTAAT              
BalI         3023  TGGCCA               TGG^CCA
             3701  TGGCCA              
BanII        2237  GAGCTC               G[GA]GC[CT]^C
             5500  GAGCTC              
BbsI         3851  GAAGAC               GAAGAC(2/6)
             1624  GTCTTC              
BciVI        3689  GGATAC               GTATCC(6/5)
             3495  GTATCC              
BfrI         2899  CTTAAG               C^TTAAG
             4990  CTTAAG              
BglII        2773  AGATCT               A^GATCT
             5531  AGATCT              
BpuAI        3851  GAAGAC               GAAGAC(2/6)
             1624  GTCTTC              
BsaBI         677  GATTGGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3472  GATGGCTATC          
BsaWI        1100  ACCGGT               [AT]^CCGG[AT]
             5588  TCCGGA              
BsgI           53  GTGCAG               GTGCAG(16/14)
             4617  GTGCAG              
BsmI         3271  GAATGC               GAATGC(1/-1)
             5546  GCATTC              
Bsp1407I     1054  TGTACA               T^GTACA
             3013  TGTACA              
BspMI        3339  ACCTGC               ACCTGC(4/8)
             1733  GCAGGT              
BsrDI        1329  CATTGC               GCAATG(2/0)
             3056  GCAATG              
BsrGI        1054  TGTACA               T^GTACA
             3013  TGTACA              
Bsu36I       3283  CCTAAGG              CC^T[ACGT]AGG
             5448  CCTCAGG             
CfoI          907  GCGC                 GCG^C
             1761  GCGC                
EaeI         3023  TGGCCA               [CT]^GGCC[GA]
             3701  TGGCCA              
Eco81I       3283  CCTAAGG              CC^T[ACGT]AGG
             5448  CCTCAGG             
EcoRV        2873  GATATC               GAT^ATC
             4919  GATATC              
FauI          225  CCCGC                CCCGC(4/6)
             1257  CCCGC               
HhaI          907  GCGC                 GCG^C
             1761  GCGC                
HinP1I        907  GCGC                 G^CGC
             1761  GCGC                
HincII       1000  GTTAAC               GT[CT]^[GA]AC
             4254  GTCGAC              
HindII       1000  GTTAAC               GT[CT]^[GA]AC
             4254  GTCGAC              
MamI          677  GATTGGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3472  GATGGCTATC          
MfeI         3132  CAATTG               C^AATTG
             3456  CAATTG              
MluNI        3023  TGGCCA               TGG^CCA
             3701  TGGCCA              
MscI         3023  TGGCCA               TGG^CCA
             3701  TGGCCA              
MunI         3132  CAATTG               C^AATTG
             3456  CAATTG              
NdeI          687  CATATG               CA^TATG
             1304  CATATG              
PaeR7I       1917  CTCGAG               C^TCGAG
             2445  CTCGAG              
PpuMI        1294  AGGTCCT              [GA]G^G[AT]CC[CT]
             5760  AGGTCCT             
PshBI         577  ATTAAT               AT^TAAT
             4747  ATTAAT              
SacI         2237  GAGCTC               GAGCT^C
             5500  GAGCTC              
SapI         1865  GAAGAGC              GCTCTTC(1/4)
             3734  GAAGAGC             
ScaI         4329  AGTACT               AGT^ACT
             4846  AGTACT              
SmaI           74  CCCGGG               CCC^GGG
             2141  CCCGGG              
SpeI          628  ACTAGT               A^CTAGT
             5406  ACTAGT              
SspBI        1054  TGTACA               T^GTACA
             3013  TGTACA              
StuI         2903  AGGCCT               AGG^CCT
             3593  AGGCCT              
Tsp45I       5377  GTCAC                ^GT[GC]AC
             5643  GTCAC               
XbaI         1108  TCTAGA               T^CTAGA
             2282  TCTAGA              
XhoI         1917  CTCGAG               C^TCGAG
             2445  CTCGAG              
XmaCI          74  CCCGGG               C^CCGGG
             2141  CCCGGG              
XmaI           74  CCCGGG               C^CCGGG
             2141  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
Alw44I      AlwI        AlwNI       ApaLI       ApoI        
Asp700I     AspHI       AvaI        AvaII       BamHI       
BanI        BbvI        BclI        BcnI        BfaI        
BlpI        BmyI        BpmI        Bpu1102I    BsaI        
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspHI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CelII       Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EarI        EcoO109I    EcoRI       
EcoRII      EcoT14I     EcoT22I     FbaI        Fnu4HI      
FokI        HaeIII      HapII       HindIII     HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NsiI        NspI        PflMI       
PleI        PstI        PvuII       RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       XmnI