##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BglI
BlnI BlpI Bpu1102I BsaBI BsaHI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BssHII Bst1107I BstUI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KspI MamI
MluI MroI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SgrAI SnaBI SphI Sse8387I
StuI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 3003 GGTACC G^GTACC
AccI 3196 GTCTAC GT^[AC][GT]AC
AflIII 3357 ACGTGT A^C[GA][CT]GT
AlwI 3780 GGATC GGATC(4/5)
AocI 2238 CCTTAGG CC^T[ACGT]AGG
Asp718I 3003 GGTACC G^GTACC
AspHI 2719 GAGCTC G[AT]GC[AT]^C
BcgI 3163 GCATAGCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2931 GTATCC GTATCC(6/5)
BclI 1457 TGATCA T^GATCA
BsaWI 2079 TCCGGT [AT]^CCGG[AT]
BsiHKAI 2719 GAGCTC G[AT]GC[AT]^C
BspHI 633 TCATGA T^CATGA
BssSI 3443 CTCGTG CACGAG(-5/-1)
BstAPI 997 GCAGCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 38 TTCGAA TT^CGAA
BstEII 3709 GGTGACC G^GT[ACGT]ACC
BstPI 3709 GGTGACC G^GT[ACGT]ACC
BstXI 257 CCAAAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2238 CCTTAGG CC^T[ACGT]AGG
DraIII 2941 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2238 CCTTAGG CC^T[ACGT]AGG
EcoO65I 3709 GGTGACC G^GT[ACGT]ACC
EcoRI 3421 GAATTC G^AATTC
FbaI 1457 TGATCA T^GATCA
KpnI 3003 GGTACC GGTAC^C
MfeI 3502 CAATTG C^AATTG
MunI 3502 CAATTG C^AATTG
NdeI 2211 CATATG CA^TATG
NspI 961 GCATGT [GA]CATG^[CT]
NspV 38 TTCGAA TT^CGAA
PpuMI 1646 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 995 CTGCAG CTGCA^G
RcaI 633 TCATGA T^CATGA
SacI 2719 GAGCTC GAGCT^C
SapI 1048 GCTCTTC GCTCTTC(1/4)
SexAI 1604 ACCAGGT A^CC[AT]GGT
SfuI 38 TTCGAA TT^CGAA
SmaI 1668 CCCGGG CCC^GGG
SpeI 1189 ACTAGT A^CTAGT
SspI 1060 AATATT AAT^ATT
SwaI 1063 ATTTAAAT ATTT^AAAT
TfiI 3 GAATC G^A[AT]TC
XbaI 1326 TCTAGA T^CTAGA
XmaCI 1668 CCCGGG C^CCGGG
XmaI 1668 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 1495 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
2848 CAGTTACTG
ApaI 157 GGGCCC GGGCC^C
1671 GGGCCC
BalI 890 TGGCCA TGG^CCA
1845 TGGCCA
BanI 1369 GGCACC G^G[CT][GA]CC
3003 GGTACC
BbsI 505 GAAGAC GAAGAC(2/6)
1185 GAAGAC
BglII 764 AGATCT A^GATCT
3363 AGATCT
BpuAI 505 GAAGAC GAAGAC(2/6)
1185 GAAGAC
BsaAI 3356 TACGTG [CT]AC^GT[GA]
3539 TACGTG
BsaI 1716 GAGACC GGTCTC(1/5)
1747 GAGACC
BsgI 2683 CTGCAC GTGCAG(16/14)
2407 GTGCAG
BsmI 957 GAATGC GAATGC(1/-1)
1359 GCATTC
BspMI 2034 GCAGGT ACCTGC(4/8)
2992 GCAGGT
BstYI 764 AGATCT [GA]^GATC[CT]
3363 AGATCT
EcoT22I 959 ATGCAT ATGCA^T
2830 ATGCAT
MflI 764 AGATCT [GA]^GATC[CT]
3363 AGATCT
MluNI 890 TGGCCA TGG^CCA
1845 TGGCCA
MscI 890 TGGCCA TGG^CCA
1845 TGGCCA
NsiI 959 ATGCAT ATGCA^T
2830 ATGCAT
PflMI 2085 CCATTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2847 CCAGTTACTGG
PvuII 1988 CAGCTG CAG^CTG
2680 CAGCTG
ScaI 2954 AGTACT AGT^ACT
2980 AGTACT
Tsp45I 1679 GTGAC ^GT[GC]AC
3710 GTGAC
Van91I 2085 CCATTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2847 CCAGTTACTGG
XhoII 764 AGATCT [GA]^GATC[CT]
3363 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
ApoI AseI AsnI Asp700I AvaI
AvaII BanII BbvI BcnI BfaI
BfrI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BsrGI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I XcmI XmnI