##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BalI BamHI BbrPI
BbvCI BciVI BclI BglI BlnI
BsaAI BsaBI BsaI BsiEI BsiWI
BspDI BspLU11I BsrDI BsrFI BssHII
BssSI BstAPI BstBI Bsu36I Cfr10I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco52I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI KspI MamI MfeI
MluI MluNI MscI MunI NaeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Van91I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 1994 TCCGGA T^CCGGA
AflII 1610 CTTAAG C^TTAAG
AlwNI 1189 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1987 AGCGCT AGC^GCT
AspHI 2432 GTGCTC G[AT]GC[AT]^C
BbeI 142 GGCGCC GGCGC^C
BfaI 2472 CTAG C^TAG
BfrI 1610 CTTAAG C^TTAAG
BsaWI 1994 TCCGGA [AT]^CCGG[AT]
BseAI 1994 TCCGGA T^CCGGA
BsiHKAI 2432 GTGCTC G[AT]GC[AT]^C
BspEI 1994 TCCGGA T^CCGGA
BspHI 1177 TCATGA T^CATGA
BspMI 2097 ACCTGC ACCTGC(4/8)
BsrBI 206 GAGCGG CCGCTC(-3/-3)
Bst1107I 545 GTATAC GTA^TAC
BstEII 2463 GGTGACC G^GT[ACGT]ACC
BstPI 2463 GGTGACC G^GT[ACGT]ACC
BstZ17I 545 GTATAC GTA^TAC
DrdI 1568 GACACTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 975 TGGCCG [CT]^GGCC[GA]
Eco47III 1987 AGCGCT AGC^GCT
EcoNI 282 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2463 GGTGACC G^GT[ACGT]ACC
KasI 142 GGCGCC G^GCGCC
MaeI 2472 CTAG C^TAG
MaeII 2363 ACGT A^CGT
MroI 1994 TCCGGA T^CCGGA
MspA1I 215 CAGCGG C[AC]G^C[GT]G
NarI 142 GGCGCC GG^CGCC
NcoI 2328 CCATGG C^CATGG
NspI 1968 GCATGT [GA]CATG^[CT]
RcaI 1177 TCATGA T^CATGA
SfoI 142 GGCGCC GGC^GCC
StuI 1361 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccI 545 GTATAC GT^[AC][GT]AC
1037 GTAGAC
AccII 75 CGCG CG^CG
521 CGCG
AcyI 6 GACGCC G[GA]^CG[CT]C
142 GGCGCC
AspI 1321 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1498 GACCAAGTC
BanII 1202 GAGCCC G[GA]GC[CT]^C
2084 GGGCTC
BcgI 123 CGATGCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2091 CGAAGGACCTGC
BglII 507 AGATCT A^GATCT
960 AGATCT
BlpI 1886 GCTGAGC GC^T[ACGT]AGC
2077 GCTTAGC
Bpu1102I 1886 GCTGAGC GC^T[ACGT]AGC
2077 GCTTAGC
BsaHI 6 GACGCC G[GA]^CG[CT]C
142 GGCGCC
BseRI 97 CTCCTC GAGGAG(10/8)
2104 GAGGAG
BsgI 781 GTGCAG GTGCAG(16/14)
945 GTGCAG
BsmBI 4 GAGACG CGTCTC(1/5)
71 GAGACG
BsmI 397 GAATGC GAATGC(1/-1)
2696 GAATGC
Bsp1407I 2654 TGTACA T^GTACA
2674 TGTACA
BsrGI 2654 TGTACA T^GTACA
2674 TGTACA
BstUI 75 CGCG CG^CG
521 CGCG
BstXI 1935 CCAAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2139 CCATACCTCTGG
BstYI 507 AGATCT [GA]^GATC[CT]
960 AGATCT
CelII 1886 GCTGAGC GC^T[ACGT]AGC
2077 GCTTAGC
DraI 470 TTTAAA TTT^AAA
922 TTTAAA
DsaI 870 CCACGG C^C[GA][CT]GG
2328 CCATGG
FauI 482 GCGGG CCCGC(4/6)
2082 GCGGG
Hin1I 6 GACGCC G[GA]^CG[CT]C
142 GGCGCC
HincII 1085 GTTGAC GT[CT]^[GA]AC
1565 GTTGAC
HindII 1085 GTTGAC GT[CT]^[GA]AC
1565 GTTGAC
MflI 507 AGATCT [GA]^GATC[CT]
960 AGATCT
MslI 536 CATGCCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1324 CATGTCGATG
MvnI 75 CGCG CG^CG
521 CGCG
NdeI 1863 CATATG CA^TATG
2670 CATATG
PaeR7I 226 CTCGAG C^TCGAG
884 CTCGAG
PflFI 1321 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1498 GACCAAGTC
SexAI 1059 ACCAGGT A^CC[AT]GGT
1601 ACCAGGT
SspBI 2654 TGTACA T^GTACA
2674 TGTACA
SspI 152 AATATT AAT^ATT
1299 AATATT
TfiI 436 GATTC G^A[AT]TC
2185 GAATC
Tth111I 1321 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1498 GACCAAGTC
XhoI 226 CTCGAG C^TCGAG
884 CTCGAG
XhoII 507 AGATCT [GA]^GATC[CT]
960 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaI AvaII BanI
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaJI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XmnI