##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
BalI BciVI BglII BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BspLU11I BsrFI BssHII BssSI Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I FseI FspI HpaI KpnI
KspI MluI MluNI MroI MscI
NaeI NgoMIV NotI NruI NspV
PacI PaeR7I PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 4864 GTAGAC GT^[AC][GT]AC
AflII 3743 CTTAAG C^TTAAG
AflIII 3436 ACGTGT A^C[GA][CT]GT
AocI 613 CCTCAGG CC^T[ACGT]AGG
ApaI 1084 GGGCCC GGGCC^C
Asp700I 3804 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 107 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2835 CCTAGG C^CTAGG
BamHI 4486 GGATCC G^GATCC
BbeI 1373 GGCGCC GGCGC^C
BfrI 3743 CTTAAG C^TTAAG
BglI 94 GCCAAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2835 CCTAGG C^CTAGG
BlpI 5113 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 5113 GCTTAGC GC^T[ACGT]AGC
BsaBI 1172 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 4170 GAGACG CGTCTC(1/5)
BspMI 3697 GCAGGT ACCTGC(4/8)
BsrBI 499 GAGCGG CCGCTC(-3/-3)
BstXI 307 CCAGATGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 613 CCTCAGG CC^T[ACGT]AGG
CelII 5113 GCTTAGC GC^T[ACGT]AGC
DraII 4535 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 1210 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 613 CCTCAGG CC^T[ACGT]AGG
EcoO109I 4535 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1239 GATATC GAT^ATC
HincII 4016 GTTGAC GT[CT]^[GA]AC
HindII 4016 GTTGAC GT[CT]^[GA]AC
KasI 1373 GGCGCC G^GCGCC
MamI 1172 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1373 GGCGCC GG^CGCC
NdeI 3910 CATATG CA^TATG
NheI 2116 GCTAGC G^CTAGC
PflFI 107 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 1389 GAGCTC GAGCT^C
SexAI 2013 ACCTGGT A^CC[AT]GGT
SfoI 1373 GGCGCC GGC^GCC
Tth111I 107 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1633 TCTAGA T^CTAGA
XcmI 2944 CCAGGTGTGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3804 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
BbrPI 1416 CACGTG CAC^GTG
3435 CACGTG
BbvCI 2529 GCTGAGG CCTCAGC(-5/-2)
5204 GCTGAGG
BcgI 44 GCATGAGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4739 GCAGTGGCGTCG
BsaI 1075 GAGACC GGTCTC(1/5)
3415 GGTCTC
BsmI 2552 GCATTC GAATGC(1/-1)
4750 GCATTC
Bsp1407I 3078 TGTACA T^GTACA
4155 TGTACA
BsrGI 3078 TGTACA T^GTACA
4155 TGTACA
BstAPI 1567 GCATGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3222 GCAATTGCTGC
BstEII 146 GGTCACC G^GT[ACGT]ACC
4641 GGTCACC
BstPI 146 GGTCACC G^GT[ACGT]ACC
4641 GGTCACC
BstYI 785 AGATCC [GA]^GATC[CT]
4486 GGATCC
EaeI 936 TGGCCG [CT]^GGCC[GA]
2581 CGGCCA
EcoNI 670 CCTCCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1984 CCTCCAAGAGG
EcoO65I 146 GGTCACC G^GT[ACGT]ACC
4641 GGTCACC
EcoRI 2705 GAATTC G^AATTC
5177 GAATTC
HaeII 1373 GGCGCC [GA]GCGC^[CT]
2214 GGCGCT
MfeI 3223 CAATTG C^AATTG
4734 CAATTG
MflI 785 AGATCC [GA]^GATC[CT]
4486 GGATCC
MspA1I 2797 CAGCTG C[AC]G^C[GT]G
3450 CAGCTG
MunI 3223 CAATTG C^AATTG
4734 CAATTG
NcoI 2059 CCATGG C^CATGG
2519 CCATGG
PflMI 66 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1415 CCACGTGCTGG
PmaCI 1416 CACGTG CAC^GTG
3435 CACGTG
PmlI 1416 CACGTG CAC^GTG
3435 CACGTG
PvuII 2797 CAGCTG CAG^CTG
3450 CAGCTG
SpeI 460 ACTAGT A^CTAGT
3035 ACTAGT
SspBI 3078 TGTACA T^GTACA
4155 TGTACA
StuI 2694 AGGCCT AGG^CCT
3362 AGGCCT
Van91I 66 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1415 CCACGTGCTGG
XhoII 785 AGATCC [GA]^GATC[CT]
4486 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BclI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EarI EcoRII EcoT14I
EcoT22I FauI FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PleI PstI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I