##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspHI AspI
AviII BamHI BanII BbeI BbrPI
BbvCI BcgI BclI BglI BglII
BpmI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII BssSI BstBI
BstEII BstPI Bsu36I Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI Sse8387I SspBI
StuI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AflII 1333 CTTAAG C^TTAAG
AflIII 1248 ACATGT A^C[GA][CT]GT
AlwI 256 GATCC GGATC(4/5)
AlwNI 110 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 704 ATTAAT AT^TAAT
AsnI 704 ATTAAT AT^TAAT
Asp700I 729 GAACCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 359 GGACC G^G[AT]CC
AvrII 1660 CCTAGG C^CTAGG
BalI 626 TGGCCA TGG^CCA
BanI 2045 GGTGCC G^G[CT][GA]CC
BciVI 752 GGATAC GTATCC(6/5)
BfrI 1333 CTTAAG C^TTAAG
BlnI 1660 CCTAGG C^CTAGG
BlpI 1274 GCTTAGC GC^T[ACGT]AGC
BmyI 2046 GTGCCC G[AGT]GC[ACT]^C
Bpu1102I 1274 GCTTAGC GC^T[ACGT]AGC
BseRI 2018 CTCCTC GAGGAG(10/8)
BsmBI 64 GAGACG CGTCTC(1/5)
Bsp1286I 2046 GTGCCC G[AGT]GC[ACT]^C
BspHI 482 TCATGA T^CATGA
BspLU11I 1248 ACATGT A^CATGT
BsrDI 718 CATTGC GCAATG(2/0)
Bst1107I 1471 GTATAC GTA^TAC
BstXI 95 CCAACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 255 AGATCC [GA]^GATC[CT]
BstZ17I 1471 GTATAC GTA^TAC
CelII 1274 GCTTAGC GC^T[ACGT]AGC
EaeI 626 TGGCCA [CT]^GGCC[GA]
EcoRI 941 GAATTC G^AATTC
FauI 3 GCGGG CCCGC(4/6)
HaeII 330 AGCGCC [GA]GCGC^[CT]
HgaI 416 GACGC GACGC(5/10)
MaeII 2098 ACGT A^CGT
MflI 255 AGATCC [GA]^GATC[CT]
MluNI 626 TGGCCA TGG^CCA
MscI 626 TGGCCA TGG^CCA
NdeI 2059 CATATG CA^TATG
PshBI 704 ATTAAT AT^TAAT
PstI 107 CTGCAG CTGCA^G
RcaI 482 TCATGA T^CATGA
SmaI 724 CCCGGG CCC^GGG
SpeI 1242 ACTAGT A^CTAGT
SphI 1430 GCATGC GCATG^C
XbaI 252 TCTAGA T^CTAGA
XcmI 209 CCATCATGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 255 AGATCC [GA]^GATC[CT]
XmaCI 724 CCCGGG C^CCGGG
XmaI 724 CCCGGG C^CCGGG
XmnI 729 GAACCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 1471 GTATAC GT^[AC][GT]AC
1783 GTCTAC
AccII 859 CGCG CG^CG
873 CGCG
AhdI 114 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
524 GACAACAAGTC
AspEI 114 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
524 GACAACAAGTC
AvaI 61 CCCGAG C^[CT]CG[GA]G
724 CCCGGG
BcnI 724 CCCGG CC^[GC]GG
725 CCGGG
BsoBI 61 CCCGAG C^[CT]CG[GA]G
724 CCCGGG
BstAPI 109 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
713 GCACCCATTGC
BstF5I 57 CATCC GGATG(2/0)
142 GGATG
BstNI 194 CCTGG CC^[AT]GG
583 CCTGG
BstUI 859 CGCG CG^CG
873 CGCG
Cfr13I 359 GGACC G^G[ACGT]CC
1889 GGGCC
Eam1105I 114 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
524 GACAACAAGTC
EcoRII 194 CCTGG ^CC[AT]GG
583 CCTGG
EcoT14I 1660 CCTAGG C^C[AT][AT]GG
1804 CCTTGG
FokI 57 CATCC GGATG(9/13)
142 GGATG
HapII 272 CCGG C^CGG
725 CCGG
HpaII 272 CCGG C^CGG
725 CCGG
MspA1I 133 CAGCTG C[AC]G^C[GT]G
1453 CAGCTG
MspI 272 CCGG C^CGG
725 CCGG
MvaI 194 CCTGG CC^[AT]GG
583 CCTGG
MvnI 859 CGCG CG^CG
873 CGCG
NciI 724 CCCGG CC^[GC]GG
725 CCGGG
NspI 1248 ACATGT [GA]CATG^[CT]
1430 GCATGC
PflMI 350 CCAATAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1046 CCATGTAGTGG
PshAI 1487 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1526 GACAGCAGTC
PvuII 133 CAGCTG CAG^CTG
1453 CAGCTG
Sau96I 359 GGACC G^G[ACGT]CC
1889 GGGCC
SfaNI 156 GATGC GCATC(5/9)
56 GCATC
SmlI 1333 CTTAAG C^T[CT][GA]AG
1757 CTTGAG
SspI 1550 AATATT AAT^ATT
2027 AATATT
StyI 1660 CCTAGG C^C[AT][AT]GG
1804 CCTTGG
TaqI 414 TCGA T^CGA
1362 TCGA
TthHB8I 414 TCGA T^CGA
1362 TCGA
Van91I 350 CCAATAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1046 CCATGTAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbsI BbvI BfaI BpuAI BsaI
BsaJI BsiYI BslI BsmAI BsmFI
BspMI BsrI BssKI Cac8I CfoI
DdeI DpnII EarI Fnu4HI HaeIII
HhaI HinP1I HindIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MslI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI ScrFI SfcI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI