##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI Aor51HI AscI Asp718I AviII
BalI BamHI BbeI BbrPI BcgI
BglI BlpI Bpu1102I BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BspEI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
CelII Cfr10I CpoI EaeI EagI
EclXI Eco47III Eco52I FseI FspI
HaeII Hin1I HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NgoMIV
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I StuI SwaI Van91I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 26 GTCTAC GT^[AC][GT]AC
AccII 31 CGCG CG^CG
AhdI 5531 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1160 GTGCAC G^TGCAC
AlwNI 194 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 5504 CCTCAGG CC^T[ACGT]AGG
ApaLI 1160 GTGCAC G^TGCAC
AspEI 5531 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3065 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 11 CCCGGG C^[CT]CG[GA]G
AvrII 3721 CCTAGG C^CTAGG
BanI 2857 GGCACC G^G[CT][GA]CC
BlnI 3721 CCTAGG C^CTAGG
BsaAI 3113 TACGTG [CT]AC^GT[GA]
BsaBI 3430 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 11 CCCGGG C^[CT]CG[GA]G
BspLU11I 3644 ACATGT A^CATGT
BspMI 5709 GCAGGT ACCTGC(4/8)
BstEII 4299 GGTCACC G^GT[ACGT]ACC
BstPI 4299 GGTCACC G^GT[ACGT]ACC
BstUI 31 CGCG CG^CG
Bsu36I 5504 CCTCAGG CC^T[ACGT]AGG
CfoI 32 GCGC GCG^C
DrdI 4328 GACAACTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 323 CCATGG C^C[GA][CT]GG
Eam1105I 5531 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 5504 CCTCAGG CC^T[ACGT]AGG
EcoO65I 4299 GGTCACC G^GT[ACGT]ACC
EcoRV 910 GATATC GAT^ATC
FauI 4529 GCGGG CCCGC(4/6)
HapII 12 CCGG C^CGG
HgaI 4383 GACGC GACGC(5/10)
HhaI 32 GCGC GCG^C
HinP1I 32 GCGC G^CGC
HpaII 12 CCGG C^CGG
MamI 3430 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 12 CCGG C^CGG
MvnI 31 CGCG CG^CG
NcoI 323 CCATGG C^CATGG
NheI 4225 GCTAGC G^CTAGC
PflFI 3065 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 203 GACCCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 4725 CTGCAG CTGCA^G
ScaI 5741 AGTACT AGT^ACT
SmaI 11 CCCGGG CCC^GGG
Tth111I 3065 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3944 CCAGAATCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 11 CCCGGG C^CCGGG
XmaI 11 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 6 GCGG CCGC(-3/-1)
4529 GCGG
AflII 4230 CTTAAG C^TTAAG
4831 CTTAAG
AflIII 3114 ACGTGT A^C[GA][CT]GT
3644 ACATGT
ApaI 3802 GGGCCC GGGCC^C
3892 GGGCCC
AspHI 758 GAGCAC G[AT]GC[AT]^C
1160 GTGCAC
BbsI 2418 GAAGAC GAAGAC(2/6)
4244 GAAGAC
BbvCI 4523 GCTGAGG CCTCAGC(-5/-2)
4657 GCTGAGG
BciVI 5495 GGATAC GTATCC(6/5)
3491 GTATCC
BclI 376 TGATCA T^GATCA
4004 TGATCA
BcnI 11 CCCGG CC^[GC]GG
12 CCGGG
BfrI 4230 CTTAAG C^TTAAG
4831 CTTAAG
BglII 3212 AGATCT A^GATCT
3652 AGATCT
BpmI 239 CTGGAG CTGGAG(16/14)
3933 CTGGAG
BpuAI 2418 GAAGAC GAAGAC(2/6)
4244 GAAGAC
BsaI 3565 GAGACC GGTCTC(1/5)
4556 GAGACC
BsiHKAI 758 GAGCAC G[AT]GC[AT]^C
1160 GTGCAC
BspDI 3038 ATCGAT AT^CGAT
4168 ATCGAT
BspHI 718 TCATGA T^CATGA
1861 TCATGA
ClaI 3038 ATCGAT AT^CGAT
4168 ATCGAT
DraIII 2505 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
3096 CACTCTGTG
EcoNI 796 CCTTGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4191 CCTTGAAAAGG
FbaI 376 TGATCA T^GATCA
4004 TGATCA
MaeII 3114 ACGT A^CGT
3468 ACGT
NciI 11 CCCGG CC^[GC]GG
12 CCGGG
PleI 143 GAGTC GAGTC(4/5)
712 GAGTC
RcaI 718 TCATGA T^CATGA
1861 TCATGA
SapI 317 GAAGAGC GCTCTTC(1/4)
4097 GAAGAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AseI AsnI Asp700I AvaII BanII
BbvI BfaI BmyI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRI EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HincII
HindII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NdeI NdeII NlaIII
NlaIV NsiI NspI PshBI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI