##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AgeI        Aor51HI     AscI        Asp718I     AviII       
BalI        BamHI       BbeI        BbrPI       BcgI        
BglI        BlpI        Bpu1102I    BsaHI       BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
BspEI       BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstXI       BstZ17I     
CelII       Cfr10I      CpoI        EaeI        EagI        
EclXI       Eco47III    Eco52I      FseI        FspI        
HaeII       Hin1I       HpaI        KasI        KpnI        
KspI        MfeI        MluI        MluNI       MroI        
MscI        MunI        NaeI        NarI        NgoMIV      
NotI        NruI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    StuI        SwaI        Van91I      XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI           26  GTCTAC               GT^[AC][GT]AC
AccII          31  CGCG                 CG^CG
AhdI         5531  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1160  GTGCAC               G^TGCAC
AlwNI         194  CAGCACCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         5504  CCTCAGG              CC^T[ACGT]AGG
ApaLI        1160  GTGCAC               G^TGCAC
AspEI        5531  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         3065  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI           11  CCCGGG               C^[CT]CG[GA]G
AvrII        3721  CCTAGG               C^CTAGG
BanI         2857  GGCACC               G^G[CT][GA]CC
BlnI         3721  CCTAGG               C^CTAGG
BsaAI        3113  TACGTG               [CT]AC^GT[GA]
BsaBI        3430  GATATAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI          11  CCCGGG               C^[CT]CG[GA]G
BspLU11I     3644  ACATGT               A^CATGT
BspMI        5709  GCAGGT               ACCTGC(4/8)
BstEII       4299  GGTCACC              G^GT[ACGT]ACC
BstPI        4299  GGTCACC              G^GT[ACGT]ACC
BstUI          31  CGCG                 CG^CG
Bsu36I       5504  CCTCAGG              CC^T[ACGT]AGG
CfoI           32  GCGC                 GCG^C
DrdI         4328  GACAACTCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          323  CCATGG               C^C[GA][CT]GG
Eam1105I     5531  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       5504  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      4299  GGTCACC              G^GT[ACGT]ACC
EcoRV         910  GATATC               GAT^ATC
FauI         4529  GCGGG                CCCGC(4/6)
HapII          12  CCGG                 C^CGG
HgaI         4383  GACGC                GACGC(5/10)
HhaI           32  GCGC                 GCG^C
HinP1I         32  GCGC                 G^CGC
HpaII          12  CCGG                 C^CGG
MamI         3430  GATATAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI           12  CCGG                 C^CGG
MvnI           31  CGCG                 CG^CG
NcoI          323  CCATGG               C^CATGG
NheI         4225  GCTAGC               G^CTAGC
PflFI        3065  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI         203  GACCCTAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         4725  CTGCAG               CTGCA^G
ScaI         5741  AGTACT               AGT^ACT
SmaI           11  CCCGGG               CCC^GGG
Tth111I      3065  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         3944  CCAGAATCAAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI          11  CCCGGG               C^CCGGG
XmaI           11  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AciI            6  GCGG                 CCGC(-3/-1)
             4529  GCGG                
AflII        4230  CTTAAG               C^TTAAG
             4831  CTTAAG              
AflIII       3114  ACGTGT               A^C[GA][CT]GT
             3644  ACATGT              
ApaI         3802  GGGCCC               GGGCC^C
             3892  GGGCCC              
AspHI         758  GAGCAC               G[AT]GC[AT]^C
             1160  GTGCAC              
BbsI         2418  GAAGAC               GAAGAC(2/6)
             4244  GAAGAC              
BbvCI        4523  GCTGAGG              CCTCAGC(-5/-2)
             4657  GCTGAGG             
BciVI        5495  GGATAC               GTATCC(6/5)
             3491  GTATCC              
BclI          376  TGATCA               T^GATCA
             4004  TGATCA              
BcnI           11  CCCGG                CC^[GC]GG
               12  CCGGG               
BfrI         4230  CTTAAG               C^TTAAG
             4831  CTTAAG              
BglII        3212  AGATCT               A^GATCT
             3652  AGATCT              
BpmI          239  CTGGAG               CTGGAG(16/14)
             3933  CTGGAG              
BpuAI        2418  GAAGAC               GAAGAC(2/6)
             4244  GAAGAC              
BsaI         3565  GAGACC               GGTCTC(1/5)
             4556  GAGACC              
BsiHKAI       758  GAGCAC               G[AT]GC[AT]^C
             1160  GTGCAC              
BspDI        3038  ATCGAT               AT^CGAT
             4168  ATCGAT              
BspHI         718  TCATGA               T^CATGA
             1861  TCATGA              
ClaI         3038  ATCGAT               AT^CGAT
             4168  ATCGAT              
DraIII       2505  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3096  CACTCTGTG           
EcoNI         796  CCTTGAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4191  CCTTGAAAAGG         
FbaI          376  TGATCA               T^GATCA
             4004  TGATCA              
MaeII        3114  ACGT                 A^CGT
             3468  ACGT                
NciI           11  CCCGG                CC^[GC]GG
               12  CCGGG               
PleI          143  GAGTC                GAGTC(4/5)
              712  GAGTC               
RcaI          718  TCATGA               T^CATGA
             1861  TCATGA              
SapI          317  GAAGAGC              GCTCTTC(1/4)
             4097  GAAGAGC             


#########################################
# 3 OR MORE CUT ENZYMES (   91 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
AseI        AsnI        Asp700I     AvaII       BanII       
BbvI        BfaI        BmyI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    Bsp1407I    BsrDI       BsrGI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraI        DraII       
EarI        EcoO109I    EcoRI       EcoRII      EcoT14I     
EcoT22I     Fnu4HI      FokI        HaeIII      HincII      
HindII      HindIII     HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MvaI        MwoI        NdeI        NdeII       NlaIII      
NlaIV       NsiI        NspI        PshBI       PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SphI        SspBI       SspI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
XmnI