##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
Acc65I AccIII AclI AflIII AgeI
AscI Asp718I AviII BamHI BcgI
BglI BsaWI BseAI BsiWI BspEI
BspLU11I BsrBI BsrDI BssHII BssSI
Bst1107I BstBI BstZ17I CpoI EcoRI
EcoT22I FseI FspI HindIII KpnI
KspI MfeI MluI MroI MunI
NdeI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmeI
Psp1406I RsrII SacII SalI SfiI
SfuI SgrAI SnaBI SpeI SphI
StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 2536 GACGTC GACGT^C
AccI 6 GTCTAC GT^[AC][GT]AC
AhdI 1620 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 195 CCTGAGG CC^T[ACGT]AGG
AseI 4110 ATTAAT AT^TAAT
AsnI 4110 ATTAAT AT^TAAT
AspEI 1620 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1269 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1764 CCTAGG C^CTAGG
BbeI 1010 GGCGCC GGCGC^C
BbvCI 978 CCTCAGC CCTCAGC(-5/-2)
BclI 1135 TGATCA T^GATCA
BglII 835 AGATCT A^GATCT
BlnI 1764 CCTAGG C^CTAGG
BlpI 24 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 24 GCTAAGC GC^T[ACGT]AGC
BsaBI 1295 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 4010 TGTACA T^GTACA
BspDI 1167 ATCGAT AT^CGAT
BspMI 1060 ACCTGC ACCTGC(4/8)
BsrGI 4010 TGTACA T^GTACA
BstAPI 1055 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1783 GGTAACC G^GT[ACGT]ACC
BstPI 1783 GGTAACC G^GT[ACGT]ACC
Bsu36I 195 CCTGAGG CC^T[ACGT]AGG
CelII 24 GCTAAGC GC^T[ACGT]AGC
ClaI 1167 ATCGAT AT^CGAT
DrdI 1023 GACGAGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 46 CGGCCG C^GGCCG
Eam1105I 1620 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 46 CGGCCG C^GGCCG
Eco52I 46 CGGCCG C^GGCCG
Eco81I 195 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1783 GGTAACC G^GT[ACGT]ACC
EcoRV 1113 GATATC GAT^ATC
FauI 76 GCGGG CCCGC(4/6)
FbaI 1135 TGATCA T^GATCA
HincII 2345 GTTAAC GT[CT]^[GA]AC
HindII 2345 GTTAAC GT[CT]^[GA]AC
HpaI 2345 GTTAAC GTT^AAC
KasI 1010 GGCGCC G^GCGCC
MamI 1295 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1010 GGCGCC GG^CGCC
NcoI 1015 CCATGG C^CATGG
PflFI 1269 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 4110 ATTAAT AT^TAAT
PvuI 1646 CGATCG CGAT^CG
SapI 2944 GCTCTTC GCTCTTC(1/4)
ScaI 3114 AGTACT AGT^ACT
SexAI 2277 ACCTGGT A^CC[AT]GGT
SfoI 1010 GGCGCC GGC^GCC
SmaI 499 CCCGGG CCC^GGG
SspBI 4010 TGTACA T^GTACA
SspI 3234 AATATT AAT^ATT
Tth111I 1269 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3365 TCTAGA T^CTAGA
XmaCI 499 CCCGGG C^CCGGG
XmaI 499 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 1010 GGCGCC G[GA]^CG[CT]C
2536 GACGTC
AfaI 3115 GTAC GT^AC
4011 GTAC
AflII 852 CTTAAG C^TTAAG
3791 CTTAAG
Alw44I 220 GTGCAC G^TGCAC
3774 GTGCAC
Aor51HI 234 AGCGCT AGC^GCT
564 AGCGCT
ApaI 1631 GGGCCC GGGCC^C
2013 GGGCCC
ApaLI 220 GTGCAC G^TGCAC
3774 GTGCAC
Asp700I 3036 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3422 GAACCAATTC
AvaI 499 CCCGGG C^[CT]CG[GA]G
1656 CCCGAG
BbsI 656 GAAGAC GAAGAC(2/6)
3557 GAAGAC
BciVI 582 GGATAC GTATCC(6/5)
1760 GTATCC
BfrI 852 CTTAAG C^TTAAG
3791 CTTAAG
BpuAI 656 GAAGAC GAAGAC(2/6)
3557 GAAGAC
BsaHI 1010 GGCGCC G[GA]^CG[CT]C
2536 GACGTC
BsiEI 46 CGGCCG CG[GA][CT]^CG
1646 CGATCG
BsmBI 2534 GAGACG CGTCTC(1/5)
3891 GAGACG
BsoBI 499 CCCGGG C^[CT]CG[GA]G
1656 CCCGAG
BspHI 649 TCATGA T^CATGA
3946 TCATGA
BstXI 1549 CCATCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4346 CCAAAGAGATGG
DraIII 2276 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
2959 CACTAGGTG
DsaI 1015 CCATGG C^C[GA][CT]GG
1682 CCACGG
Eco47III 234 AGCGCT AGC^GCT
564 AGCGCT
HgaI 15 GACGC GACGC(5/10)
3893 GACGC
Hin1I 1010 GGCGCC G[GA]^CG[CT]C
2536 GACGTC
NaeI 44 GCCGGC GCC^GGC
1374 GCCGGC
NgoMIV 44 GCCGGC G^CCGGC
1374 GCCGGC
NheI 1409 GCTAGC G^CTAGC
3533 GCTAGC
PshAI 3216 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3909 GACTTGAGTC
RcaI 649 TCATGA T^CATGA
3946 TCATGA
RsaI 3115 GTAC GT^AC
4011 GTAC
SbfI 248 CCTGCAGG CCTGCA^GG
1061 CCTGCAGG
Sse8387I 248 CCTGCAGG CCTGCA^GG
1061 CCTGCAGG
XcmI 784 CCAAGTCTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2851 CCAGAAAATGGATGG
XmnI 3036 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3422 GAACCAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
AlwNI ApoI AspHI AvaII BalI
BanI BanII BbrPI BbvI BcnI
BfaI BmyI BpmI BsaAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII