##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflIII AgeI AhdI AlwNI
ApaI AscI Asp718I AspEI AviII
AvrII BamHI BbeI BbrPI BciVI
BglI BlnI BsaAI BsaBI BsaHI
BsiWI BspDI BspLU11I BsrDI BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Cfr10I ClaI
CpoI Eam1105I EcoO65I EcoRV EcoT22I
FseI FspI Hin1I HindIII KasI
KpnI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AflII 2106 CTTAAG C^TTAAG
AseI 2451 ATTAAT AT^TAAT
AsnI 2451 ATTAAT AT^TAAT
AspI 2365 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 902 GAGCCC G[GA]GC[CT]^C
BbvCI 379 GCTGAGG CCTCAGC(-5/-2)
BcgI 1147 CGAAGACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2106 CTTAAG C^TTAAG
BglII 1560 AGATCT A^GATCT
BlpI 977 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 977 GCTCAGC GC^T[ACGT]AGC
BsgI 720 GTGCAG GTGCAG(16/14)
BsiEI 742 CGGCCG CG[GA][CT]^CG
BsmBI 804 GAGACG CGTCTC(1/5)
BsmI 958 GAATGC GAATGC(1/-1)
Bsp1407I 455 TGTACA T^GTACA
BspHI 1164 TCATGA T^CATGA
BspMI 1047 GCAGGT ACCTGC(4/8)
BsrBI 824 GAGCGG CCGCTC(-3/-3)
BsrGI 455 TGTACA T^GTACA
BssSI 1014 CTCGTG CACGAG(-5/-1)
CelII 977 GCTCAGC GC^T[ACGT]AGC
DraI 1565 TTTAAA TTT^AAA
EagI 742 CGGCCG C^GGCCG
EclXI 742 CGGCCG C^GGCCG
Eco52I 742 CGGCCG C^GGCCG
EcoNI 1903 CCTTGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1574 GAATTC G^AATTC
HgaI 806 GACGC GACGC(5/10)
HincII 1082 GTTAAC GT[CT]^[GA]AC
HindII 1082 GTTAAC GT[CT]^[GA]AC
HpaI 1082 GTTAAC GTT^AAC
NcoI 2556 CCATGG C^CATGG
NspI 438 GCATGT [GA]CATG^[CT]
PflFI 2365 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 646 CCAGCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2451 ATTAAT AT^TAAT
RcaI 1164 TCATGA T^CATGA
ScaI 2121 AGTACT AGT^ACT
SmaI 2019 CCCGGG CCC^GGG
SspBI 455 TGTACA T^GTACA
StuI 2046 AGGCCT AGG^CCT
Tth111I 2365 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 646 CCAGCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2019 CCCGGG C^CCGGG
XmaI 2019 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 97 TCCGGA T^CCGGA
1423 TCCGGA
Alw44I 854 GTGCAC G^TGCAC
1722 GTGCAC
AlwI 95 GATCC GGATC(4/5)
1183 GATCC
AocI 607 CCTCAGG CC^T[ACGT]AGG
2497 CCTCAGG
Aor51HI 648 AGCGCT AGC^GCT
2013 AGCGCT
ApaLI 854 GTGCAC G^TGCAC
1722 GTGCAC
Asp700I 509 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1510 GAAATGTTTC
AvaI 70 CTCGGG C^[CT]CG[GA]G
2019 CCCGGG
BalI 567 TGGCCA TGG^CCA
643 TGGCCA
BanI 697 GGTGCC G^G[CT][GA]CC
1538 GGTGCC
BclI 1161 TGATCA T^GATCA
1206 TGATCA
BfaI 1344 CTAG C^TAG
1988 CTAG
BseAI 97 TCCGGA T^CCGGA
1423 TCCGGA
BsoBI 70 CTCGGG C^[CT]CG[GA]G
2019 CCCGGG
BspEI 97 TCCGGA T^CCGGA
1423 TCCGGA
Bsu36I 607 CCTCAGG CC^T[ACGT]AGG
2497 CCTCAGG
DraIII 627 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
857 CACACTGTG
DsaI 906 CCGTGG C^C[GA][CT]GG
2556 CCATGG
Eco47III 648 AGCGCT AGC^GCT
2013 AGCGCT
Eco81I 607 CCTCAGG CC^T[ACGT]AGG
2497 CCTCAGG
FauI 131 CCCGC CCCGC(4/6)
298 CCCGC
FbaI 1161 TGATCA T^GATCA
1206 TGATCA
HaeII 648 AGCGCT [GA]GCGC^[CT]
2013 AGCGCT
MaeI 1344 CTAG C^TAG
1988 CTAG
MluNI 567 TGGCCA TGG^CCA
643 TGGCCA
MroI 97 TCCGGA T^CCGGA
1423 TCCGGA
MscI 567 TGGCCA TGG^CCA
643 TGGCCA
PvuII 797 CAGCTG CAG^CTG
1067 CAGCTG
SexAI 115 ACCTGGT A^CC[AT]GGT
786 ACCTGGT
TaqI 1139 TCGA T^CGA
1146 TCGA
TthHB8I 1139 TCGA T^CGA
1146 TCGA
XmnI 509 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1510 GAAATGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DrdI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII