##################################
# NO CUT ENZYMES (   55 enzymes) #
##################################

AgeI        AhdI        ApaI        AscI        AspEI       
AviII       BbeI        BglI        BsgI        BsiWI       
Bsp1407I    BspLU11I    BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstZ17I     Cfr10I      CpoI        Eam1105I    EcoO65I     
FseI        FspI        HpaI        KasI        KspI        
NaeI        NarI        NdeI        NgoMIV      NotI        
NruI        PaeR7I      PinAI       PmeI        PshAI       
PvuI        RsrII       SacII       SalI        SbfI        
SfiI        SfoI        SgrAI       SmaI        SnaBI       
Sse8387I    SspBI       XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AatII         228  GACGTC               GACGT^C
Acc65I       4605  GGTACC               G^GTACC
AccI          350  GTCTAC               GT^[AC][GT]AC
AccIII       4075  TCCGGA               T^CCGGA
AclI         4290  AACGTT               AA^CGTT
AcyI          228  GACGTC               G[GA]^CG[CT]C
Alw44I       5858  GTGCAC               G^TGCAC
AocI         1995  CCTTAGG              CC^T[ACGT]AGG
Aor51HI      2592  AGCGCT               AGC^GCT
ApaLI        5858  GTGCAC               G^TGCAC
Asp718I      4605  GGTACC               G^GTACC
AspI         1437  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        2710  GGATCC               G^GATCC
BbrPI        6207  CACGTG               CAC^GTG
BlpI          917  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I      917  GCTAAGC              GC^T[ACGT]AGC
BsaAI        6207  CACGTG               [CT]AC^GT[GA]
BsaBI        1403  GATCTCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         228  GACGTC               G[GA]^CG[CT]C
BseAI        4075  TCCGGA               T^CCGGA
BsiEI         164  CGGCCG               CG[GA][CT]^CG
BspDI        3702  ATCGAT               AT^CGAT
BspEI        4075  TCCGGA               T^CCGGA
BstBI        4925  TTCGAA               TT^CGAA
Bsu36I       1995  CCTTAGG              CC^T[ACGT]AGG
CelII         917  GCTAAGC              GC^T[ACGT]AGC
ClaI         3702  ATCGAT               AT^CGAT
EagI          164  CGGCCG               C^GGCCG
EclXI         164  CGGCCG               C^GGCCG
Eco47III     2592  AGCGCT               AGC^GCT
Eco52I        164  CGGCCG               C^GGCCG
Eco81I       1995  CCTTAGG              CC^T[ACGT]AGG
EcoT22I      6039  ATGCAT               ATGCA^T
HgaI         3161  GCGTC                GACGC(5/10)
Hin1I         228  GACGTC               G[GA]^CG[CT]C
KpnI         4605  GGTACC               GGTAC^C
MamI         1403  GATCTCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1556  CAATTG               C^AATTG
MluI         3159  ACGCGT               A^CGCGT
MroI         4075  TCCGGA               T^CCGGA
MunI         1556  CAATTG               C^AATTG
NcoI          489  CCATGG               C^CATGG
NheI         3487  GCTAGC               G^CTAGC
NsiI         6039  ATGCAT               ATGCA^T
NspV         4925  TTCGAA               TT^CGAA
PacI         1754  TTAATTAA             TTAAT^TAA
PflFI        1437  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        6207  CACGTG               CAC^GTG
PmlI         6207  CACGTG               CAC^GTG
Psp1406I     4290  AACGTT               AA^CGTT
SacI         1469  GAGCTC               GAGCT^C
SapI          875  GAAGAGC              GCTCTTC(1/4)
SexAI        1710  ACCTGGT              A^CC[AT]GGT
SfuI         4925  TTCGAA               TT^CGAA
SspI         1586  AATATT               AAT^ATT
SwaI         4164  ATTTAAAT             ATTT^AAAT
Tth111I      1437  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         5701  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AflII        3865  CTTAAG               C^TTAAG
             5966  CTTAAG              
AflIII       3159  ACGCGT               A^C[GA][CT]GT
             6206  ACACGT              
Asp700I       370  GAACTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5693  GAAGCATTTC          
AvrII         295  CCTAGG               C^CTAGG
             4766  CCTAGG              
BbvCI          97  CCTCAGC              CCTCAGC(-5/-2)
             2705  GCTGAGG             
BfrI         3865  CTTAAG               C^TTAAG
             5966  CTTAAG              
BglII         304  AGATCT               A^GATCT
             3333  AGATCT              
BlnI          295  CCTAGG               C^CTAGG
             4766  CCTAGG              
BsaWI        4075  TCCGGA               [AT]^CCGG[AT]
             4311  TCCGGT              
BsmBI         226  GAGACG               CGTCTC(1/5)
             4399  GAGACG              
BspHI        3493  TCATGA               T^CATGA
             5019  TCATGA              
BstAPI        770  GCATTACATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1256  GCAAACAGTGC         
DraIII       2049  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             6264  CACATTGTG           
DrdI         1197  GACAGAGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4198  GACGGAAGTGTC        
HaeII        2592  AGCGCT               [GA]GCGC^[CT]
             3484  GGCGCT              
RcaI         3493  TCATGA               T^CATGA
             5019  TCATGA              
SpeI         4630  ACTAGT               A^CTAGT
             5773  ACTAGT              
SphI          290  GCATGC               GCATG^C
             3823  GCATGC              
StuI          610  AGGCCT               AGG^CCT
             2362  AGGCCT              
XcmI          315  CCACCACAGAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3117  CCACCAAGTTCATGG     
XmnI          370  GAACTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5693  GAAGCATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  124 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AseI        AsnI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvI        BcgI        BciVI       
BclI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspMI       BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EarI        EcoNI       EcoO109I    EcoRI       EcoRII      
EcoRV       EcoT14I     FauI        FbaI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HincII      HindII      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PshBI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScaI        ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII