##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        AspEI       AviII       
BamHI       BbsI        BbvCI       BcgI        BciVI       
BclI        BpuAI       BsaBI       BseAI       BsiEI       
BsiWI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRV       EcoT22I     FbaI        FseI        FspI        
HpaI        MamI        MfeI        MluI        MroI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PinAI       
PmeI        PshBI       Psp1406I    PvuI        RsrII       
SalI        SbfI        ScaI        SexAI       SfuI        
SgrAI       SnaBI       SphI        Sse8387I    SspBI       
SspI        SwaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I        682  GGTACC               G^GTACC
AflII         962  CTTAAG               C^TTAAG
AflIII        911  ACACGT               A^C[GA][CT]GT
Asp700I      1736  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       682  GGTACC               G^GTACC
AspHI           8  GAGCTC               G[AT]GC[AT]^C
AspI         2247  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII          16  CCTAGG               C^CTAGG
BfrI          962  CTTAAG               C^TTAAG
BglII        1636  AGATCT               A^GATCT
BlnI           16  CCTAGG               C^CTAGG
BsaWI        1558  TCCGGT               [AT]^CCGG[AT]
BsiHKAI         8  GAGCTC               G[AT]GC[AT]^C
BsmI         1507  GCATTC               GAATGC(1/-1)
BspHI        2390  TCATGA               T^CATGA
BspMI         129  ACCTGC               ACCTGC(4/8)
BsrFI         820  GCCGGC               [GA]^CCGG[CT]
BssSI         883  CACGAG               CACGAG(-5/-1)
BstXI        2221  CCATCGCCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        820  GCCGGC               [GA]^CCGG[CT]
EcoRI         875  GAATTC               G^AATTC
HincII       1980  GTCAAC               GT[CT]^[GA]AC
HindII       1980  GTCAAC               GT[CT]^[GA]AC
HindIII       959  AAGCTT               A^AGCTT
KpnI          682  GGTACC               GGTAC^C
KspI           68  CCGCGG               CCGC^GG
NaeI          820  GCCGGC               GCC^GGC
NgoMIV        820  GCCGGC               G^CCGGC
PflFI        2247  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI         2390  TCATGA               T^CATGA
SacI            8  GAGCTC               GAGCT^C
SacII          68  CCGCGG               CCGC^GG
SapI         2487  GCTCTTC              GCTCTTC(1/4)
SmaI         1303  CCCGGG               CCC^GGG
SpeI         1592  ACTAGT               A^CTAGT
StuI         1708  AGGCCT               AGG^CCT
Tth111I      2247  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          479  TCTAGA               T^CTAGA
XmaCI        1303  CCCGGG               C^CCGGG
XmaI         1303  CCCGGG               C^CCGGG
XmnI         1736  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcsI          432  AAATTC               [GA]^AATT[CT]
              875  GAATTC              
AfaI          683  GTAC                 GT^AC
             1088  GTAC                
ApoI          432  AAATTC               [GA]^AATT[CT]
              875  GAATTC              
BbrPI         912  CACGTG               CAC^GTG
             1404  CACGTG              
BlpI          815  GCTGAGC              GC^T[ACGT]AGC
             2375  GCTGAGC             
Bpu1102I      815  GCTGAGC              GC^T[ACGT]AGC
             2375  GCTGAGC             
BsmBI        1125  CGTCTC               CGTCTC(1/5)
             2316  GAGACG              
BsrDI        1262  CATTGC               GCAATG(2/0)
             1435  GCAATG              
BstAPI        651  GCAGGAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1192  GCAAGTCCTGC         
CelII         815  GCTGAGC              GC^T[ACGT]AGC
             2375  GCTGAGC             
DraIII       1404  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2402  CACGCTGTG           
EcoNI         521  CCTGGTCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1202  CCTCCTCCAGG         
MslI         1661  CATGGCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2222  CATCGCCATG          
PaeR7I        673  CTCGAG               C^TCGAG
             2191  CTCGAG              
PmaCI         912  CACGTG               CAC^GTG
             1404  CACGTG              
PmlI          912  CACGTG               CAC^GTG
             1404  CACGTG              
PshAI         518  GACCCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1119  GACCTTCGTC          
RsaI          683  GTAC                 GT^AC
             1088  GTAC                
SfiI          149  GGCCTCCAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             2417  GGCCCACGGGGCC       
TfiI         1715  GATTC                G^A[AT]TC
             1763  GATTC               
XhoI          673  CTCGAG               C^TCGAG
             2191  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
AlwNI       ApaI        AvaI        AvaII       BalI        
BanI        BanII       BbeI        BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BsaAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NarI        NciI        NcoI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PpuMI       PstI        
PvuII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmlI        StyI        TaqI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII