##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
Acc65I AccIII AclI AfaI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AvrII
BbrPI BciVI BclI BfrI BglII
BlnI BsaBI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraI Eco47III
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
MamI MfeI MluI MroI MseI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsaI RsrII SalI
SapI SbfI ScaI SexAI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 432 GACGTC GACGT^C
AccI 270 GTAGAC GT^[AC][GT]AC
AcsI 868 AAATTT [GA]^AATT[CT]
AflIII 1386 ACATGT A^C[GA][CT]GT
AhdI 618 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 868 AAATTT [GA]^AATT[CT]
AspEI 618 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1532 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 570 CCCGAG C^[CT]CG[GA]G
AviII 604 TGCGCA TGC^GCA
BamHI 2275 GGATCC G^GATCC
BbeI 576 GGCGCC GGCGC^C
BcgI 1951 GCAGTGGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 1970 TACGTG [CT]AC^GT[GA]
BsmBI 776 CGTCTC CGTCTC(1/5)
BsmI 2251 GAATGC GAATGC(1/-1)
BsoBI 570 CCCGAG C^[CT]CG[GA]G
BspHI 1519 TCATGA T^CATGA
BspLU11I 1386 ACATGT A^CATGT
DrdI 1295 GACAGAACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 618 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1890 CTCTTC CTCTTC(1/4)
FspI 604 TGCGCA TGC^GCA
HaeII 576 GGCGCC [GA]GCGC^[CT]
KasI 576 GGCGCC G^GCGCC
Ksp632I 1890 CTCTTC CTCTTC(1/4)
KspI 747 CCGCGG CCGC^GG
NaeI 760 GCCGGC GCC^GGC
NarI 576 GGCGCC GG^CGCC
NgoMIV 760 GCCGGC G^CCGGC
PflFI 1532 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1519 TCATGA T^CATGA
SacII 747 CCGCGG CCGC^GG
SfaNI 1526 GATGC GCATC(5/9)
SfiI 792 GGCCTGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 576 GGCGCC GGC^GCC
Tsp509I 869 AATT ^AATT
Tth111I 1532 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AocI 803 CCTTAGG CC^T[ACGT]AGG
1035 CCTGAGG
BbsI 1341 GTCTTC GAAGAC(2/6)
1644 GTCTTC
BbvCI 1055 CCTCAGC CCTCAGC(-5/-2)
1942 GCTGAGG
BcnI 12 CCCGG CC^[GC]GG
1490 CCCGG
BlpI 557 GCTCAGC GC^T[ACGT]AGC
1229 GCTGAGC
Bpu1102I 557 GCTCAGC GC^T[ACGT]AGC
1229 GCTGAGC
BpuAI 1341 GTCTTC GAAGAC(2/6)
1644 GTCTTC
BsaI 1986 GAGACC GGTCTC(1/5)
386 GGTCTC
BsiEI 758 CGGCCG CG[GA][CT]^CG
1210 CGGCCG
BsrFI 666 GCCGGT [GA]^CCGG[CT]
760 GCCGGC
BstEII 317 GGTGACC G^GT[ACGT]ACC
338 GGTGACC
BstPI 317 GGTGACC G^GT[ACGT]ACC
338 GGTGACC
BstYI 1311 AGATCC [GA]^GATC[CT]
2275 GGATCC
Bsu36I 803 CCTTAGG CC^T[ACGT]AGG
1035 CCTGAGG
CelII 557 GCTCAGC GC^T[ACGT]AGC
1229 GCTGAGC
Cfr10I 666 GCCGGT [GA]^CCGG[CT]
760 GCCGGC
DraIII 1075 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
1925 CACCCCGTG
EagI 758 CGGCCG C^GGCCG
1210 CGGCCG
EclXI 758 CGGCCG C^GGCCG
1210 CGGCCG
Eco52I 758 CGGCCG C^GGCCG
1210 CGGCCG
Eco81I 803 CCTTAGG CC^T[ACGT]AGG
1035 CCTGAGG
EcoO65I 317 GGTGACC G^GT[ACGT]ACC
338 GGTGACC
MflI 1311 AGATCC [GA]^GATC[CT]
2275 GGATCC
NciI 12 CCCGG CC^[GC]GG
1490 CCCGG
NspI 978 ACATGC [GA]CATG^[CT]
1386 ACATGT
PflMI 1074 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1283 CCAGTCCATGG
SmlI 644 CTTGAG C^T[CT][GA]AG
1803 CTTGAG
StuI 791 AGGCCT AGG^CCT
1039 AGGCCT
TaqI 409 TCGA T^CGA
1410 TCGA
TthHB8I 409 TCGA T^CGA
1410 TCGA
Van91I 1074 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1283 CCAGTCCATGG
XhoII 1311 AGATCC [GA]^GATC[CT]
2275 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI ApaI AspHI AvaII BalI
BanI BanII BbvI BfaI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NcoI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII SacI Sau3AI Sau96I
ScrFI SfcI StyI TseI Tsp45I
TspRI XcmI