##################################
# NO CUT ENZYMES (   54 enzymes) #
##################################

AclI        AgeI        Aor51HI     AscI        AseI        
AsnI        BamHI       BsiWI       Bsp1407I    BspDI       
BsrGI       BssHII      Bst1107I    BstBI       BstZ17I     
ClaI        CpoI        DrdI        EagI        EclXI       
Eco47III    Eco52I      EcoT22I     FseI        HincII      
HindII      HpaI        MluI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PinAI       PmeI        PshBI       
Psp1406I    PvuI        RsrII       SalI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
SpeI        SphI        SspBI       StuI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AatII        3867  GACGTC               GACGT^C
AccIII        582  TCCGGA               T^CCGGA
AflII        3462  CTTAAG               C^TTAAG
AhdI         4552  GACACTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         4652  CCTGAGG              CC^T[ACGT]AGG
AspEI        4552  GACACTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         5235  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1926  TGCGCA               TGC^GCA
AvrII        5106  CCTAGG               C^CTAGG
BbeI          817  GGCGCC               GGCGC^C
BcgI         2538  CGATGGGCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         3462  CTTAAG               C^TTAAG
BlnI         5106  CCTAGG               C^CTAGG
BlpI         4191  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     4191  GCTCAGC              GC^T[ACGT]AGC
BsaWI         582  TCCGGA               [AT]^CCGG[AT]
BseAI         582  TCCGGA               T^CCGGA
BsiEI        2291  CGGTCG               CG[GA][CT]^CG
BspEI         582  TCCGGA               T^CCGGA
BsrFI         505  GCCGGT               [GA]^CCGG[CT]
BssSI        4690  CTCGTG               CACGAG(-5/-1)
BstEII       1201  GGTAACC              G^GT[ACGT]ACC
BstPI        1201  GGTAACC              G^GT[ACGT]ACC
Bsu36I       4652  CCTGAGG              CC^T[ACGT]AGG
CelII        4191  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        505  GCCGGT               [GA]^CCGG[CT]
DraIII       5000  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     4552  GACACTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       4652  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      1201  GGTAACC              G^GT[ACGT]ACC
EcoRV        1781  GATATC               GAT^ATC
FspI         1926  TGCGCA               TGC^GCA
HgaI         2428  GACGC                GACGC(5/10)
HindIII      3737  AAGCTT               A^AGCTT
KasI          817  GGCGCC               G^GCGCC
MfeI         4511  CAATTG               C^AATTG
MroI          582  TCCGGA               T^CCGGA
MunI         4511  CAATTG               C^AATTG
NarI          817  GGCGCC               GG^CGCC
PaeR7I       4624  CTCGAG               C^TCGAG
PflFI        5235  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SacI         3500  GAGCTC               GAGCT^C
SbfI         4603  CCTGCAGG             CCTGCA^GG
SfoI          817  GGCGCC               GGC^GCC
Sse8387I     4603  CCTGCAGG             CCTGCA^GG
SspI         3149  AATATT               AAT^ATT
SwaI         4417  ATTTAAAT             ATTT^AAAT
Tth111I      5235  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          844  TCTAGA               T^CTAGA
XhoI         4624  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

Acc65I        830  GGTACC               G^GTACC
             2400  GGTACC              
AccI         1886  GTCTAC               GT^[AC][GT]AC
             3366  GTCTAC              
AcyI          817  GGCGCC               G[GA]^CG[CT]C
             3867  GACGTC              
Alw44I       1148  GTGCAC               G^TGCAC
             4693  GTGCAC              
ApaI         2973  GGGCCC               GGGCC^C
             5138  GGGCCC              
ApaLI        1148  GTGCAC               G^TGCAC
             4693  GTGCAC              
Asp700I      1981  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3616  GAAGCACTTC          
Asp718I       830  GGTACC               G^GTACC
             2400  GGTACC              
BbvCI        2635  GCTGAGG              CCTCAGC(-5/-2)
             5168  GCTGAGG             
BciVI         525  GGATAC               GTATCC(6/5)
             3192  GTATCC              
BglII        4182  AGATCT               A^GATCT
             5029  AGATCT              
BsaBI         206  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3308  GATTAAAATC          
BsaHI         817  GGCGCC               G[GA]^CG[CT]C
             3867  GACGTC              
BspLU11I     2115  ACATGT               A^CATGT
             5541  ACATGT              
BsrBI         786  GAGCGG               CCGCTC(-3/-3)
             2252  GAGCGG              
BsrDI        3384  CATTGC               GCAATG(2/0)
             5385  CATTGC              
DraI         4044  TTTAAA               TTT^AAA
             4418  TTTAAA              
EcoRI        1315  GAATTC               G^AATTC
             4680  GAATTC              
HaeII         817  GGCGCC               [GA]GCGC^[CT]
             2348  AGCGCC              
Hin1I         817  GGCGCC               G[GA]^CG[CT]C
             3867  GACGTC              
KpnI          830  GGTACC               GGTAC^C
             2400  GGTACC              
KspI         1969  CCGCGG               CCGC^GG
             2396  CCGCGG              
MamI          206  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3308  GATTAAAATC          
PshAI        1251  GACAGATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1879  GACCTACGTC          
SacII        1969  CCGCGG               CCGC^GG
             2396  CCGCGG              
SapI          634  GCTCTTC              GCTCTTC(1/4)
             1408  GCTCTTC             
SmaI         2049  CCCGGG               CCC^GGG
             2977  CCCGGG              
XmaCI        2049  CCCGGG               C^CCGGG
             2977  CCCGGG              
XmaI         2049  CCCGGG               C^CCGGG
             2977  CCCGGG              
XmnI         1981  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3616  GAAGCACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  124 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbrPI       BbsI        BbvI        BclI        BcnI        
BfaI        BglI        BmyI        BpmI        BpuAI       
BsaAI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspHI       
BspMI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        FbaI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PmaCI       
PmlI        PpuMI       PstI        PvuII       RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII