##################################
# NO CUT ENZYMES (   60 enzymes) #
##################################

AatII       Alw44I      Aor51HI     ApaLI       AscI        
AviII       BamHI       BglII       BlpI        Bpu1102I    
BsaBI       BsiWI       BsmBI       BsmI        BspDI       
BssHII      Bst1107I    BstEII      BstPI       BstZ17I     
CelII       ClaI        CpoI        Eco47III    EcoNI       
EcoO65I     EcoRV       EcoT22I     FseI        FspI        
MamI        MfeI        MluI        MunI        NcoI        
NotI        NruI        NsiI        PacI        PaeR7I      
PmeI        PshAI       PvuI        RsrII       SalI        
SbfI        SexAI       SfiI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    StuI        
SwaI        XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   69 enzymes) #
###################################

Acc65I       2959  GGTACC               G^GTACC
AccIII       1497  TCCGGA               T^CCGGA
AclI          372  AACGTT               AA^CGTT
AcyI         1463  GGCGCC               G[GA]^CG[CT]C
AflII         420  CTTAAG               C^TTAAG
AgeI         3681  ACCGGT               A^CCGGT
AhdI          882  GACTCACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1378  CCTCAGG              CC^T[ACGT]AGG
ApaI         1480  GGGCCC               GGGCC^C
Asp700I      1179  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2959  GGTACC               G^GTACC
AspEI         882  GACTCACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1665  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII         576  CCTAGG               C^CTAGG
BbeI         1463  GGCGCC               GGCGC^C
BcgI         1088  CGAAGATTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2909  GTATCC               GTATCC(6/5)
BfrI          420  CTTAAG               C^TTAAG
BglI         1455  GCCTCCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          576  CCTAGG               C^CTAGG
BsaHI        1463  GGCGCC               G[GA]^CG[CT]C
BseAI        1497  TCCGGA               T^CCGGA
BseRI        2493  GAGGAG               GAGGAG(10/8)
BsiEI        1446  CGGCCG               CG[GA][CT]^CG
BsmFI        3043  GGGAC                GGGAC(10/14)
Bsp1407I     1219  TGTACA               T^GTACA
BspEI        1497  TCCGGA               T^CCGGA
BspHI        1061  TCATGA               T^CATGA
BspMI        3068  GCAGGT               ACCTGC(4/8)
BsrBI         341  GAGCGG               CCGCTC(-3/-3)
BsrGI        1219  TGTACA               T^GTACA
BssSI        3010  CTCGTG               CACGAG(-5/-1)
BstAPI       2933  GCAGACACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         308  TTCGAA               TT^CGAA
BstXI        2097  CCAAGTCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1378  CCTCAGG              CC^T[ACGT]AGG
DrdI         1662  GACGACGGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1446  CGGCCG               C^GGCCG
Eam1105I      882  GACTCACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1446  CGGCCG               C^GGCCG
Eco52I       1446  CGGCCG               C^GGCCG
Eco81I       1378  CCTCAGG              CC^T[ACGT]AGG
EcoRI        2325  GAATTC               G^AATTC
Hin1I        1463  GGCGCC               G[GA]^CG[CT]C
HpaI          298  GTTAAC               GTT^AAC
KasI         1463  GGCGCC               G^GCGCC
KpnI         2959  GGTACC               GGTAC^C
KspI         1653  CCGCGG               CCGC^GG
MroI         1497  TCCGGA               T^CCGGA
NaeI         2440  GCCGGC               GCC^GGC
NarI         1463  GGCGCC               GG^CGCC
NdeI          286  CATATG               CA^TATG
NgoMIV       2440  GCCGGC               G^CCGGC
NheI         1451  GCTAGC               G^CTAGC
NspV          308  TTCGAA               TT^CGAA
PflFI        1665  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI          63  CCAGCCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        3681  ACCGGT               A^CCGGT
Psp1406I      372  AACGTT               AA^CGTT
PvuII         793  CAGCTG               CAG^CTG
RcaI         1061  TCATGA               T^CATGA
SacII        1653  CCGCGG               CCGC^GG
SfoI         1463  GGCGCC               GGC^GCC
SfuI          308  TTCGAA               TT^CGAA
SspBI        1219  TGTACA               T^GTACA
Tth111I      1665  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I         63  CCAGCCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1394  CCAAGAAAGCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1179  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI          781  GTCTAC               GT^[AC][GT]AC
             2195  GTCTAC              
AseI          691  ATTAAT               AT^TAAT
             2302  ATTAAT              
AsnI          691  ATTAAT               AT^TAAT
             2302  ATTAAT              
BbrPI        1897  CACGTG               CAC^GTG
             2789  CACGTG              
BbvCI        1595  CCTCAGC              CCTCAGC(-5/-2)
             2002  CCTCAGC             
BsaAI        1897  CACGTG               [CT]AC^GT[GA]
             2789  CACGTG              
BsaI         2027  GGTCTC               GGTCTC(1/5)
             2901  GGTCTC              
BsaWI        1497  TCCGGA               [AT]^CCGG[AT]
             3681  ACCGGT              
BspLU11I        4  ACATGT               A^CATGT
              302  ACATGT              
BsrDI        2429  CATTGC               GCAATG(2/0)
             2996  GCAATG              
BsrFI        2440  GCCGGC               [GA]^CCGG[CT]
             3681  ACCGGT              
Cfr10I       2440  GCCGGC               [GA]^CCGG[CT]
             3681  ACCGGT              
DraII        1382  AGGACCC              [GA]G^G[ACGT]CC[CT]
             1855  AGGACCT             
DraIII        173  CACTAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
              760  CACAAAGTG           
DsaI           68  CCGTGG               C^C[GA][CT]GG
             1653  CCGCGG              
EcoO109I     1382  AGGACCC              [GA]G^G[ACGT]CC[CT]
             1855  AGGACCT             
EcoT14I       576  CCTAGG               C^C[AT][AT]GG
             1403  CCTTGG              
HaeII        1463  GGCGCC               [GA]GCGC^[CT]
             2399  AGCGCC              
HindIII       115  AAGCTT               A^AGCTT
             1552  AAGCTT              
PmaCI        1897  CACGTG               CAC^GTG
             2789  CACGTG              
PmlI         1897  CACGTG               CAC^GTG
             2789  CACGTG              
PpuMI        1382  AGGACCC              [GA]G^G[AT]CC[CT]
             1855  AGGACCT             
PshBI         691  ATTAAT               AT^TAAT
             2302  ATTAAT              
SapI          718  GAAGAGC              GCTCTTC(1/4)
             1182  GCTCTTC             
ScaI         3581  AGTACT               AGT^ACT
             3662  AGTACT              
StyI          576  CCTAGG               C^C[AT][AT]GG
             1403  CCTTGG              


#########################################
# 3 OR MORE CUT ENZYMES (  103 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvI        BclI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsoBI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        EaeI        EarI        
EcoRII      FauI        FbaI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PstI        
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII