##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AgeI        AhdI        AlwNI       Aor51HI     AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BbeI        BbrPI       BciVI       
BclI        BfrI        BlnI        BsaAI       BsaBI       
BsaWI       BseAI       BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrDI       
BsrGI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     ClaI        CpoI        DraI        
Eam1105I    Eco47III    EcoO65I     EcoT22I     FbaI        
FseI        FspI        HpaI        KasI        KpnI        
MamI        MfeI        MluI        MroI        MslI        
MunI        NarI        NdeI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SalI        ScaI        
SfiI        SfoI        SfuI        SnaBI       SpeI        
SphI        SspBI       SspI        StuI        SwaI        
Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AflIII       1356  ACGTGT               A^C[GA][CT]GT
BalI         1332  TGGCCA               TGG^CCA
BamHI         171  GGATCC               G^GATCC
BbsI          280  GAAGAC               GAAGAC(2/6)
BcgI         2096  GCAGCCAAGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        2478  AGATCT               A^GATCT
BpuAI         280  GAAGAC               GAAGAC(2/6)
BsmBI         706  CGTCTC               CGTCTC(1/5)
BstAPI        256  GCAGTACATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII       1670  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         2779  GACCCTCGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        2550  GAATTC               G^AATTC
EcoRV        2446  GATATC               GAT^ATC
HincII       2739  GTCAAC               GT[CT]^[GA]AC
HindII       2739  GTCAAC               GT[CT]^[GA]AC
MluNI        1332  TGGCCA               TGG^CCA
MscI         1332  TGGCCA               TGG^CCA
NheI         2116  GCTAGC               G^CTAGC
NspI          261  ACATGC               [GA]CATG^[CT]
PaeR7I       2671  CTCGAG               C^TCGAG
PflMI         319  CCAGCCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1011  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI         715  GACGCCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI         1476  GCTCTTC              GCTCTTC(1/4)
SbfI         2989  CCTGCAGG             CCTGCA^GG
SexAI        2658  ACCTGGT              A^CC[AT]GGT
SgrAI        1404  CACCGGCG             C[GA]^CCGG[CT]G
SmlI         2671  CTCGAG               C^T[CT][GA]AG
Sse8387I     2989  CCTGCAGG             CCTGCA^GG
Van91I        319  CCAGCCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         2671  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AatII          54  GACGTC               GACGT^C
              895  GACGTC              
Alw44I       1556  GTGCAC               G^TGCAC
             1925  GTGCAC              
AlwI          172  GATCC                GGATC(4/5)
              171  GGATC               
AocI         1093  CCTCAGG              CC^T[ACGT]AGG
             2223  CCTCAGG             
ApaLI        1556  GTGCAC               G^TGCAC
             1925  GTGCAC              
Asp700I      2065  GAAACCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2680  GAATTTTTTC          
BanI         1030  GGTGCC               G^G[CT][GA]CC
             1402  GGCACC              
BbvCI        1210  CCTCAGC              CCTCAGC(-5/-2)
             2817  GCTGAGG             
BfaI          407  CTAG                 C^TAG
             2117  CTAG                
BsaI         2843  GAGACC               GGTCTC(1/5)
              214  GGTCTC              
BsgI         1172  CTGCAC               GTGCAG(16/14)
             2416  GTGCAG              
BstXI         957  CCAAAGTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1518  CCACCAGGCTGG        
BstYI         171  GGATCC               [GA]^GATC[CT]
             2478  AGATCT              
Bsu36I       1093  CCTCAGG              CC^T[ACGT]AGG
             2223  CCTCAGG             
DpnII         172  GATC                 ^GATC
             2479  GATC                
Eco81I       1093  CCTCAGG              CC^T[ACGT]AGG
             2223  CCTCAGG             
EcoNI        1576  CCTGTCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1950  CCTCGCTCAGG         
HindIII       519  AAGCTT               A^AGCTT
             3010  AAGCTT              
KspI          165  CCGCGG               CCGC^GG
             1422  CCGCGG              
MaeI          407  CTAG                 C^TAG
             2117  CTAG                
MboI          172  GATC                 ^GATC
             2479  GATC                
MflI          171  GGATCC               [GA]^GATC[CT]
             2478  AGATCT              
NaeI          613  GCCGGC               GCC^GGC
             1775  GCCGGC              
NdeII         172  GATC                 ^GATC
             2479  GATC                
NgoMIV        613  GCCGGC               G^CCGGC
             1775  GCCGGC              
SacII         165  CCGCGG               CCGC^GG
             1422  CCGCGG              
Sau3AI        172  GATC                 ^GATC
             2479  GATC                
SmaI          184  CCCGGG               CCC^GGG
             1901  CCCGGG              
TfiI         2614  GATTC                G^A[AT]TC
             2637  GAATC               
XhoII         171  GGATCC               [GA]^GATC[CT]
             2478  AGATCT              
XmaCI         184  CCCGGG               C^CCGGG
             1901  CCCGGG              
XmaI          184  CCCGGG               C^CCGGG
             1901  CCCGGG              
XmnI         2065  GAAACCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2680  GAATTTTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        ApaI        ApoI        AspHI       AvaI        
AvaII       BanII       BbvI        BcnI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BsaHI       
BsaJI       BseRI       BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BssSI       BstF5I      BstNI       BstUI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DraII       DsaI        EaeI        EagI        EarI        
EclXI       Eco52I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboII       MnlI        MseI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NlaIII      NlaIV       PleI        
PstI        PvuII       RsaI        SacI        Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XcmI