##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
Alw44I AlwNI AocI ApaLI AscI
Asp718I AspEI AviII AvrII BalI
BamHI BbrPI BbvCI BcgI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII EaeI EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoRV FauI FseI FspI HgaI
KpnI KspI MamI MluI MluNI
MroI MscI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacII SalI SbfI SexAI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SwaI Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 929 GACGTC GACGT^C
AclI 1705 AACGTT AA^CGTT
AflIII 526 ACATGT A^C[GA][CT]GT
Aor51HI 1434 AGCGCT AGC^GCT
ApaI 1646 GGGCCC GGGCC^C
AseI 2387 ATTAAT AT^TAAT
AsnI 2387 ATTAAT AT^TAAT
AvaI 1244 CCCGAG C^[CT]CG[GA]G
BbeI 12 GGCGCC GGCGC^C
BbsI 1556 GAAGAC GAAGAC(2/6)
BciVI 2139 GTATCC GTATCC(6/5)
BclI 654 TGATCA T^GATCA
BcnI 125 CCCGG CC^[GC]GG
BglI 1801 GCCAAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1556 GAAGAC GAAGAC(2/6)
BsgI 2376 GTGCAG GTGCAG(16/14)
BsiYI 1483 CCTTGGCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1483 CCTTGGCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 2504 GCATTC GAATGC(1/-1)
BsoBI 1244 CCCGAG C^[CT]CG[GA]G
BspHI 707 TCATGA T^CATGA
BspLU11I 526 ACATGT A^CATGT
BstXI 1781 CCAGGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 923 GACTCAGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 60 CCGTGG C^C[GA][CT]GG
Eco47III 1434 AGCGCT AGC^GCT
EcoNI 1483 CCTTGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 961 GAATTC G^AATTC
EcoT22I 2502 ATGCAT ATGCA^T
FbaI 654 TGATCA T^GATCA
HincII 2359 GTTAAC GT[CT]^[GA]AC
HindII 2359 GTTAAC GT[CT]^[GA]AC
HpaI 2359 GTTAAC GTT^AAC
KasI 12 GGCGCC G^GCGCC
NarI 12 GGCGCC GG^CGCC
NciI 125 CCCGG CC^[GC]GG
NsiI 2502 ATGCAT ATGCA^T
PpuMI 1193 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 2387 ATTAAT AT^TAAT
Psp1406I 1705 AACGTT AA^CGTT
RcaI 707 TCATGA T^CATGA
ScaI 838 AGTACT AGT^ACT
SfiI 1800 GGCCAAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 12 GGCGCC GGC^GCC
SmlI 1613 CTCAAG C^T[CT][GA]AG
StuI 1783 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 932 GTCTAC GT^[AC][GT]AC
2025 GTAGAC
AccII 39 CGCG CG^CG
79 CGCG
AciI 10 GCGG CCGC(-3/-1)
80 GCGG
AcyI 12 GGCGCC G[GA]^CG[CT]C
929 GACGTC
AspHI 1597 GAGCTC G[AT]GC[AT]^C
1762 GAGCTC
AspI 682 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1546 GACTATGTC
BanI 12 GGCGCC G^G[CT][GA]CC
1725 GGCACC
BsaHI 12 GGCGCC G[GA]^CG[CT]C
929 GACGTC
BseRI 2065 CTCCTC GAGGAG(10/8)
1131 GAGGAG
BsiHKAI 1597 GAGCTC G[AT]GC[AT]^C
1762 GAGCTC
BsmFI 955 GGGAC GGGAC(10/14)
229 GTCCC
BspMI 910 ACCTGC ACCTGC(4/8)
2079 ACCTGC
BsrDI 421 CATTGC GCAATG(2/0)
2443 GCAATG
BsrI 952 ACTGG ACTGG(1/-1)
826 CCAGT
BstUI 39 CGCG CG^CG
79 CGCG
HaeII 12 GGCGCC [GA]GCGC^[CT]
1434 AGCGCT
HapII 120 CCGG C^CGG
126 CCGG
Hin1I 12 GGCGCC G[GA]^CG[CT]C
929 GACGTC
HpaII 120 CCGG C^CGG
126 CCGG
HphI 745 GGTGA GGTGA(8/7)
1377 TCACC
MaeII 930 ACGT A^CGT
1706 ACGT
MspI 120 CCGG C^CGG
126 CCGG
MvnI 39 CGCG CG^CG
79 CGCG
PflFI 682 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1546 GACTATGTC
PleI 923 GACTC GAGTC(4/5)
1493 GACTC
SacI 1597 GAGCTC GAGCT^C
1762 GAGCTC
SphI 1048 GCATGC GCATG^C
2017 GCATGC
TaqI 369 TCGA T^CGA
1268 TCGA
Tsp45I 589 GTGAC ^GT[GC]AC
1417 GTCAC
Tth111I 682 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1546 GACTATGTC
TthHB8I 369 TCGA T^CGA
1268 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I AvaII BanII BbvI BfaI
BmyI BpmI BsaJI BsmAI Bsp1286I
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HhaI HinP1I HindIII
HinfI ItaI Ksp632I MaeI MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MunI MvaI
MwoI NdeII NlaIII NlaIV NspI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI TfiI Tru9I TseI Tsp509I
TspRI XhoII XmnI