##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BanI BbeI BbvCI BcgI
BclI BfrI BlnI BsaHI BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BsrBI BssHII Bst1107I BstEII BstPI
BstUI BstZ17I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV FbaI FseI HgaI
Hin1I HindIII KasI KpnI KspI
MluI MroI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I RsrII SacII
SalI SfiI SfoI SgrAI SnaBI
SpeI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AocI 3315 CCTGAGG CC^T[ACGT]AGG
AviII 3758 TGCGCA TGC^GCA
BamHI 732 GGATCC G^GATCC
BbrPI 2503 CACGTG CAC^GTG
BglI 755 GCCGGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1263 AGATCT A^GATCT
BsaAI 2503 CACGTG [CT]AC^GT[GA]
BsiEI 3127 CGATCG CG[GA][CT]^CG
Bsp1407I 3785 TGTACA T^GTACA
BspLU11I 335 ACATGT A^CATGT
BsrDI 608 CATTGC GCAATG(2/0)
BsrGI 3785 TGTACA T^GTACA
BssSI 2024 CTCGTG CACGAG(-5/-1)
BstBI 4486 TTCGAA TT^CGAA
Bsu36I 3315 CCTGAGG CC^T[ACGT]AGG
DraIII 299 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 3315 CCTGAGG CC^T[ACGT]AGG
EcoNI 1708 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 3186 GCGGG CCCGC(4/6)
FspI 3758 TGCGCA TGC^GCA
HaeII 965 GGCGCT [GA]GCGC^[CT]
HpaI 1013 GTTAAC GTT^AAC
MfeI 4761 CAATTG C^AATTG
MunI 4761 CAATTG C^AATTG
NspV 4486 TTCGAA TT^CGAA
PflMI 448 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2503 CACGTG CAC^GTG
PmlI 2503 CACGTG CAC^GTG
PpuMI 719 AGGTCCC [GA]G^G[AT]CC[CT]
PvuI 3127 CGATCG CGAT^CG
SapI 2935 GCTCTTC GCTCTTC(1/4)
SbfI 217 CCTGCAGG CCTGCA^GG
SexAI 199 ACCTGGT A^CC[AT]GGT
SfuI 4486 TTCGAA TT^CGAA
Sse8387I 217 CCTGCAGG CCTGCA^GG
SspBI 3785 TGTACA T^GTACA
StuI 3370 AGGCCT AGG^CCT
SwaI 4395 ATTTAAAT ATTT^AAAT
Van91I 448 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwI 733 GATCC GGATC(4/5)
732 GGATC
AlwNI 789 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2169 CAGATGCTG
BciVI 1774 GGATAC GTATCC(6/5)
2101 GGATAC
BsmI 2325 GCATTC GAATGC(1/-1)
4029 GCATTC
BsrFI 2393 GCCGGT [GA]^CCGG[CT]
3202 GCCGGT
BstAPI 2384 GCAGTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4029 GCATTCGTTGC
BstYI 732 GGATCC [GA]^GATC[CT]
1263 AGATCT
CfoI 966 GCGC GCG^C
3759 GCGC
Cfr10I 2393 GCCGGT [GA]^CCGG[CT]
3202 GCCGGT
DraII 719 AGGTCCC [GA]G^G[ACGT]CC[CT]
3282 AGGCCCT
DrdI 263 GACAGAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3736 GACTGACCTGTC
DsaI 661 CCATGG C^C[GA][CT]GG
3977 CCATGG
EcoO109I 719 AGGTCCC [GA]G^G[ACGT]CC[CT]
3282 AGGCCCT
EcoT22I 1116 ATGCAT ATGCA^T
4368 ATGCAT
HhaI 966 GCGC GCG^C
3759 GCGC
HinP1I 966 GCGC G^CGC
3759 GCGC
HincII 1013 GTTAAC GT[CT]^[GA]AC
1328 GTTGAC
HindII 1013 GTTAAC GT[CT]^[GA]AC
1328 GTTGAC
MflI 732 GGATCC [GA]^GATC[CT]
1263 AGATCT
NcoI 661 CCATGG C^CATGG
3977 CCATGG
NsiI 1116 ATGCAT ATGCA^T
4368 ATGCAT
PvuII 476 CAGCTG CAG^CTG
4039 CAGCTG
SacI 402 GAGCTC GAGCT^C
2933 GAGCTC
SmaI 16 CCCGGG CCC^GGG
2699 CCCGGG
SmlI 2930 CTTGAG C^T[CT][GA]AG
3956 CTTGAG
SphI 3761 GCATGC GCATG^C
4370 GCATGC
XbaI 1030 TCTAGA T^CTAGA
1600 TCTAGA
XhoII 732 GGATCC [GA]^GATC[CT]
1263 AGATCT
XmaCI 16 CCCGGG C^CCGGG
2699 CCCGGG
XmaI 16 CCCGGG C^CCGGG
2699 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
ApoI Asp700I AspHI AvaI AvaII
BalI BanII BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaBI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrI BssKI BstF5I BstNI BstXI
Cac8I CelII Cfr13I DdeI DpnII
DraI EaeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XmnI