##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AgeI        
Alw44I      AlwNI       ApaLI       AscI        AseI        
AsnI        Asp718I     BamHI       BbeI        BfrI        
BsaWI       BseAI       BsiWI       BspDI       BspEI       
BssSI       Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        DraI        DrdI        EcoRI       EcoT22I     
HindIII     HpaI        KasI        KpnI        KspI        
MfeI        MluI        MroI        MunI        NarI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacII       ScaI        SexAI       SfiI        
SfoI        SfuI        SmaI        SnaBI       SpeI        
SspI        SwaI        Van91I      XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AatII        2399  GACGTC               GACGT^C
AccI          650  GTCGAC               GT^[AC][GT]AC
AcsI         1793  GAATTT               [GA]^AATT[CT]
AhdI          346  GACGGCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      2129  AGCGCT               AGC^GCT
ApoI         1793  GAATTT               [GA]^AATT[CT]
Asp700I      2695  GAACAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         346  GACGGCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         2843  CTCGGG               C^[CT]CG[GA]G
AvrII         199  CCTAGG               C^CTAGG
BbvCI         919  GCTGAGG              CCTCAGC(-5/-2)
BcgI          642  GCACTCACGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1155  GTATCC               GTATCC(6/5)
BclI         1419  TGATCA               T^GATCA
BlnI          199  CCTAGG               C^CTAGG
BsaBI        2644  GATCAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        1257  GAGACG               CGTCTC(1/5)
BsmI         3354  GCATTC               GAATGC(1/-1)
BsoBI        2843  CTCGGG               C^[CT]CG[GA]G
Bsp1407I     2268  TGTACA               T^GTACA
BspHI        1492  TCATGA               T^CATGA
BspLU11I      693  ACATGT               A^CATGT
BsrGI        2268  TGTACA               T^GTACA
BssHII       2363  GCGCGC               G^CGCGC
BstAPI       1017  GCAACAAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        964  GGTGACC              G^GT[ACGT]ACC
BstPI         964  GGTGACC              G^GT[ACGT]ACC
DraIII        569  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         1101  CGGCCG               C^GGCCG
Eam1105I      346  GACGGCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1101  CGGCCG               C^GGCCG
Eco47III     2129  AGCGCT               AGC^GCT
Eco52I       1101  CGGCCG               C^GGCCG
EcoO65I       964  GGTGACC              G^GT[ACGT]ACC
EcoRV        1577  GATATC               GAT^ATC
FbaI         1419  TGATCA               T^GATCA
FseI         1102  GGCCGGCC             GGCCGG^CC
MamI         2644  GATCAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
RcaI         1492  TCATGA               T^CATGA
SalI          650  GTCGAC               G^TCGAC
SgrAI         783  CACCGGCG             C[GA]^CCGG[CT]G
SmlI         1628  CTCAAG               C^T[CT][GA]AG
SspBI        2268  TGTACA               T^GTACA
StuI         1510  AGGCCT               AGG^CCT
XmnI         2695  GAACAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AflIII        693  ACATGT               A^C[GA][CT]GT
             2154  ACACGT              
AocI          776  CCTGAGG              CC^T[ACGT]AGG
             2278  CCTGAGG             
AspI         2033  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2396  GACGACGTC           
BanI          781  GGCACC               G^G[CT][GA]CC
             1716  GGCACC              
BbrPI         755  CACGTG               CAC^GTG
             1358  CACGTG              
BglI          296  GCCTATGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3100  GCCCTCTTGGC         
BlpI          994  GCTCAGC              GC^T[ACGT]AGC
             2135  GCTGAGC             
Bpu1102I      994  GCTCAGC              GC^T[ACGT]AGC
             2135  GCTGAGC             
BsaI         1130  GAGACC               GGTCTC(1/5)
             1599  GAGACC              
BsiEI         853  CGACCG               CG[GA][CT]^CG
             1101  CGGCCG              
Bsu36I        776  CCTGAGG              CC^T[ACGT]AGG
             2278  CCTGAGG             
CelII         994  GCTCAGC              GC^T[ACGT]AGC
             2135  GCTGAGC             
Eco81I        776  CCTGAGG              CC^T[ACGT]AGG
             2278  CCTGAGG             
MseI         3378  TTAA                 T^TAA
             3385  TTAA                
NaeI         1103  GCCGGC               GCC^GGC
             1194  GCCGGC              
NcoI           57  CCATGG               C^CATGG
             1839  CCATGG              
NgoMIV       1103  GCCGGC               G^CCGGC
             1194  GCCGGC              
PflFI        2033  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2396  GACGACGTC           
PmaCI         755  CACGTG               CAC^GTG
             1358  CACGTG              
PmlI          755  CACGTG               CAC^GTG
             1358  CACGTG              
SacI          242  GAGCTC               GAGCT^C
             2681  GAGCTC              
SapI         2527  GCTCTTC              GCTCTTC(1/4)
             2683  GCTCTTC             
SbfI         1888  CCTGCAGG             CCTGCA^GG
             3189  CCTGCAGG            
SphI          420  GCATGC               GCATG^C
             2469  GCATGC              
Sse8387I     1888  CCTGCAGG             CCTGCA^GG
             3189  CCTGCAGG            
Tru9I        3378  TTAA                 T^TAA
             3385  TTAA                
Tth111I      2033  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2396  GACGACGTC           
XcmI          279  CCATCCCAGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2337  CCATGATCCGCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        ApaI        AspHI       AvaII       AviII       
BalI        BanII       BbsI        BbvI        BcnI        
BfaI        BglII       BmyI        BpmI        BpuAI       
BsaAI       BsaHI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrBI       BsrDI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        FspI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TaqI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII