##################################
# NO CUT ENZYMES (   79 enzymes) #
##################################

AatII       Acc65I      AccIII      AflIII      AgeI        
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspI        BamHI       BbrPI       
BbvCI       BcgI        BglI        BsaBI       BseAI       
BsiEI       BsiWI       BsmBI       BspDI       BspEI       
BspLU11I    BsrBI       BssHII      BssSI       Bst1107I    
BstZ17I     ClaI        CpoI        EagI        EclXI       
Eco47III    Eco52I      EcoNI       FseI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspI        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       PvuI        RsrII       
SacI        SacII       SalI        SbfI        SfiI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
StuI        SwaI        Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI         2612  GTAGAC               GT^[AC][GT]AC
AclI         1005  AACGTT               AA^CGTT
AflII        4101  CTTAAG               C^TTAAG
AlwNI        2801  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         4066  CCTAAGG              CC^T[ACGT]AGG
ApaI         3866  GGGCCC               GGGCC^C
AspHI        1880  GTGCTC               G[AT]GC[AT]^C
AvaI         2765  CCCGGG               C^[CT]CG[GA]G
AviII        2032  TGCGCA               TGC^GCA
BbsI          992  GAAGAC               GAAGAC(2/6)
BciVI        2785  GTATCC               GTATCC(6/5)
BfrI         4101  CTTAAG               C^TTAAG
BlpI         2684  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2684  GCTCAGC              GC^T[ACGT]AGC
BpuAI         992  GAAGAC               GAAGAC(2/6)
BsaAI         813  CACGTA               [CT]AC^GT[GA]
BsaWI        3042  TCCGGT               [AT]^CCGG[AT]
BsiHKAI      1880  GTGCTC               G[AT]GC[AT]^C
BsoBI        2765  CCCGGG               C^[CT]CG[GA]G
BspHI        1749  TCATGA               T^CATGA
BsrDI        4160  CATTGC               GCAATG(2/0)
BsrFI          11  GCCGGT               [GA]^CCGG[CT]
BstAPI       3191  GCACAGAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2363  GGTTACC              G^GT[ACGT]ACC
BstPI        2363  GGTTACC              G^GT[ACGT]ACC
Bsu36I       4066  CCTAAGG              CC^T[ACGT]AGG
CelII        2684  GCTCAGC              GC^T[ACGT]AGC
Cfr10I         11  GCCGGT               [GA]^CCGG[CT]
DraI         3023  TTTAAA               TTT^AAA
DrdI         2615  GACAATATTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          135  CCACGG               C^C[GA][CT]GG
Eco81I       4066  CCTAAGG              CC^T[ACGT]AGG
EcoO65I      2363  GGTTACC              G^GT[ACGT]ACC
EcoRV        1845  GATATC               GAT^ATC
FspI         2032  TGCGCA               TGC^GCA
HgaI          333  GACGC                GACGC(5/10)
HindIII      3005  AAGCTT               A^AGCTT
PpuMI        3054  GGGACCC              [GA]G^G[AT]CC[CT]
Psp1406I     1005  AACGTT               AA^CGTT
PstI         2807  CTGCAG               CTGCA^G
RcaI         1749  TCATGA               T^CATGA
ScaI         1308  AGTACT               AGT^ACT
SmaI         2765  CCCGGG               CCC^GGG
XbaI         2750  TCTAGA               T^CTAGA
XmaCI        2765  CCCGGG               C^CCGGG
XmaI         2765  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AcyI         1227  GGCGCC               G[GA]^CG[CT]C
             3032  GGCGCC              
AhdI         2754  GACCTTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3056  GACCCCCAGTC         
AspEI        2754  GACCTTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3056  GACCCCCAGTC         
AvrII        1627  CCTAGG               C^CTAGG
             3395  CCTAGG              
BalI         2128  TGGCCA               TGG^CCA
             2797  TGGCCA              
BanI         1227  GGCGCC               G^G[CT][GA]CC
             3032  GGCGCC              
BanII        3783  GAGCCC               G[GA]GC[CT]^C
             3866  GGGCCC              
BbeI         1227  GGCGCC               GGCGC^C
             3032  GGCGCC              
BglII         703  AGATCT               A^GATCT
             1265  AGATCT              
BlnI         1627  CCTAGG               C^CTAGG
             3395  CCTAGG              
BsaHI        1227  GGCGCC               G[GA]^CG[CT]C
             3032  GGCGCC              
BsaI         1131  GAGACC               GGTCTC(1/5)
             1632  GGTCTC              
BsgI         2831  CTGCAC               GTGCAG(16/14)
             3189  CTGCAC              
BstBI         363  TTCGAA               TT^CGAA
             1356  TTCGAA              
DraIII       2472  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3680  CACAATGTG           
EaeI         2128  TGGCCA               [CT]^GGCC[GA]
             2797  TGGCCA              
Eam1105I     2754  GACCTTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3056  GACCCCCAGTC         
EcoRI         360  GAATTC               G^AATTC
             2226  GAATTC              
EcoT22I      3464  ATGCAT               ATGCA^T
             4075  ATGCAT              
HaeII        1227  GGCGCC               [GA]GCGC^[CT]
             3032  GGCGCC              
Hin1I        1227  GGCGCC               G[GA]^CG[CT]C
             3032  GGCGCC              
KasI         1227  GGCGCC               G^GCGCC
             3032  GGCGCC              
MluNI        2128  TGGCCA               TGG^CCA
             2797  TGGCCA              
MscI         2128  TGGCCA               TGG^CCA
             2797  TGGCCA              
MslI         3495  CATCAGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             3538  CATTCAAATG          
NarI         1227  GGCGCC               GG^CGCC
             3032  GGCGCC              
NsiI         3464  ATGCAT               ATGCA^T
             4075  ATGCAT              
NspV          363  TTCGAA               TT^CGAA
             1356  TTCGAA              
PflMI        2471  CCACCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2789  CCATTACTTGG         
SapI         3780  GAAGAGC              GCTCTTC(1/4)
             2352  GCTCTTC             
SfoI         1227  GGCGCC               GGC^GCC
             3032  GGCGCC              
SfuI          363  TTCGAA               TT^CGAA
             1356  TTCGAA              
Van91I       2471  CCACCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2789  CCATTACTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        Asp700I     AvaII       BbvI        
BclI        BcnI        BfaI        BmyI        BpmI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    Bsp1407I    BspMI       
BsrGI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EarI        
EcoO109I    EcoRII      EcoT14I     FauI        FbaI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HincII      HindII      HinfI       HpaI        
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SexAI       SfaNI       SfcI        
SmlI        SspBI       SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII       XmnI