##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        Alw44I      
AlwNI       Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspI        AviII       AvrII       
BalI        BanI        BbeI        BbrPI       BcgI        
BciVI       BclI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmAI       BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstUI       BstZ17I     Cac8I       CelII       CfoI        
Cfr10I      ClaI        CpoI        DraIII      DrdI        
EaeI        EagI        EclXI       Eco47III    Eco52I      
EcoNI       EcoRV       FauI        FbaI        FseI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        SwaI        
TaqI        Tth111I     TthHB8I     Van91I      XcmI        
XhoI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI         1175  GTCTAC               GT^[AC][GT]AC
AhdI          431  GACAGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          418  CCTCAGG              CC^T[ACGT]AGG
ApaI          610  GGGCCC               GGGCC^C
Asp700I       304  GAATGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         431  GACAGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          613  CCCGGG               C^[CT]CG[GA]G
AvaII         438  GGTCC                G^G[AT]CC
BamHI         513  GGATCC               G^GATCC
BanII         610  GGGCCC               G[GA]GC[CT]^C
BbvCI         588  CCTCAGC              CCTCAGC(-5/-2)
BpmI          780  CTGGAG               CTGGAG(16/14)
BsoBI         613  CCCGGG               C^[CT]CG[GA]G
BspMI         162  ACCTGC               ACCTGC(4/8)
BssSI         334  CTCGTG               CACGAG(-5/-1)
BstEII         38  GGTCACC              G^GT[ACGT]ACC
BstPI          38  GGTCACC              G^GT[ACGT]ACC
Bsu36I        418  CCTCAGG              CC^T[ACGT]AGG
Eam1105I      431  GACAGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          357  GAAGAG               CTCTTC(1/4)
Eco81I        418  CCTCAGG              CC^T[ACGT]AGG
EcoO65I        38  GGTCACC              G^GT[ACGT]ACC
EcoRI        1100  GAATTC               G^AATTC
EcoT14I       696  CCATGG               C^C[AT][AT]GG
HapII         614  CCGG                 C^CGG
HgaI         1173  GCGTC                GACGC(5/10)
HincII       1424  GTTGAC               GT[CT]^[GA]AC
HindII       1424  GTTGAC               GT[CT]^[GA]AC
HpaII         614  CCGG                 C^CGG
Ksp632I       357  GAAGAG               CTCTTC(1/4)
MspI          614  CCGG                 C^CGG
NcoI          696  CCATGG               C^CATGG
NspI          388  ACATGC               [GA]CATG^[CT]
SmaI          613  CCCGGG               CCC^GGG
SspI          868  AATATT               AAT^ATT
StyI          696  CCATGG               C^C[AT][AT]GG
XbaI          202  TCTAGA               T^CTAGA
XmaCI         613  CCCGGG               C^CCGGG
XmaI          613  CCCGGG               C^CCGGG
XmnI          304  GAATGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AciI         1157  CCGC                 CCGC(-3/-1)
              837  GCGG                
AfaI          574  GTAC                 GT^AC
             1151  GTAC                
AspHI         976  GAGCAC               G[AT]GC[AT]^C
             1571  GTGCTC              
BcnI          613  CCCGG                CC^[GC]GG
              614  CCGGG               
BsiHKAI       976  GAGCAC               G[AT]GC[AT]^C
             1571  GTGCTC              
BsiYI         504  CCCCCTTCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              552  CCTGGAAACGG         
BslI          504  CCCCCTTCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              552  CCTGGAAACGG         
BsmFI         715  GGGAC                GGGAC(10/14)
              962  GTCCC               
BsmI         1440  GAATGC               GAATGC(1/-1)
             1644  GCATTC              
BstXI         471  CCAGGAAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              965  CCAGGTTTATGG        
DraI         1256  TTTAAA               TTT^AAA
             1311  TTTAAA              
DsaI          696  CCATGG               C^C[GA][CT]GG
             1753  CCACGG              
EcoT22I      1563  ATGCAT               ATGCA^T
             1737  ATGCAT              
MaeII           2  ACGT                 A^CGT
             1715  ACGT                
MslI          945  CACCCTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1740  CATTATTGTG          
NciI          613  CCCGG                CC^[GC]GG
              614  CCGGG               
NsiI         1563  ATGCAT               ATGCA^T
             1737  ATGCAT              
PleI          548  GACTC                GAGTC(4/5)
             1704  GAGTC               
PstI          349  CTGCAG               CTGCA^G
              493  CTGCAG              
RsaI          574  GTAC                 GT^AC
             1151  GTAC                
Tsp45I         39  GTCAC                ^GT[GC]AC
             1501  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AcsI        AluI        AlwI        ApoI        BbsI        
BbvI        BfaI        BglII       BmyI        BpuAI       
BsaJI       BseRI       Bsp1286I    BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cfr13I      
DdeI        DpnII       DraII       EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspA1I      MvaI        
MwoI        NdeII       NlaIII      NlaIV       PvuII       
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
XhoII