##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AviII BalI BamHI BbeI BbrPI
BbvCI BcgI BclI BcnI BfrI
BglII BlpI Bpu1102I BsaBI BseAI
BsiWI BspDI BspEI BspHI BsrBI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I CelII
ClaI CpoI DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI MamI MfeI
MflI MluI MluNI MroI MscI
MunI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I SwaI Tth111I XbaI
XcmI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 223 GTCTAC GT^[AC][GT]AC
AcsI 819 AAATTT [GA]^AATT[CT]
AcyI 36 GACGCC G[GA]^CG[CT]C
AflIII 1712 ACATGT A^C[GA][CT]GT
Alw44I 692 GTGCAC G^TGCAC
AlwNI 653 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 692 GTGCAC G^TGCAC
ApoI 819 AAATTT [GA]^AATT[CT]
AvrII 54 CCTAGG C^CTAGG
BanI 50 GGCACC G^G[CT][GA]CC
BlnI 54 CCTAGG C^CTAGG
BsaAI 1526 TACGTA [CT]AC^GT[GA]
BsaHI 36 GACGCC G[GA]^CG[CT]C
BsaWI 571 TCCGGT [AT]^CCGG[AT]
BsgI 716 CTGCAC GTGCAG(16/14)
BsmBI 34 GAGACG CGTCTC(1/5)
Bsp1407I 1600 TGTACA T^GTACA
BspLU11I 1712 ACATGT A^CATGT
BsrFI 1032 GCCGGT [GA]^CCGG[CT]
BsrGI 1600 TGTACA T^GTACA
Cfr10I 1032 GCCGGT [GA]^CCGG[CT]
FauI 24 GCGGG CCCGC(4/6)
HaeII 149 AGCGCC [GA]GCGC^[CT]
Hin1I 36 GACGCC G[GA]^CG[CT]C
KspI 180 CCGCGG CCGC^GG
NcoI 92 CCATGG C^CATGG
PvuII 752 CAGCTG CAG^CTG
SacII 180 CCGCGG CCGC^GG
ScaI 632 AGTACT AGT^ACT
SfcI 1281 CTGTAG C^T[GA][CT]AG
SnaBI 1526 TACGTA TAC^GTA
SspBI 1600 TGTACA T^GTACA
SspI 644 AATATT AAT^ATT
TaqI 876 TCGA T^CGA
Tsp45I 121 GTCAC ^GT[GC]AC
TthHB8I 876 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AocI 442 CCTGAGG CC^T[ACGT]AGG
1537 CCTTAGG
Asp700I 478 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1623 GAAGTGGTTC
AspHI 692 GTGCAC G[AT]GC[AT]^C
1194 GAGCAC
AvaI 112 CCCGAG C^[CT]CG[GA]G
229 CCCGAG
BbsI 1126 GAAGAC GAAGAC(2/6)
1210 GTCTTC
BciVI 438 GTATCC GTATCC(6/5)
929 GTATCC
BpuAI 1126 GAAGAC GAAGAC(2/6)
1210 GTCTTC
BsaI 103 GAGACC GGTCTC(1/5)
711 GGTCTC
BsiEI 7 CGGTCG CG[GA][CT]^CG
177 CGACCG
BsiHKAI 692 GTGCAC G[AT]GC[AT]^C
1194 GAGCAC
BsmFI 302 GGGAC GGGAC(10/14)
432 GGGAC
BsoBI 112 CCCGAG C^[CT]CG[GA]G
229 CCCGAG
BspMI 720 ACCTGC ACCTGC(4/8)
995 ACCTGC
BsrDI 350 GCAATG GCAATG(2/0)
1355 GCAATG
BsrI 1312 ACTGG ACTGG(1/-1)
744 CCAGT
Bsu36I 442 CCTGAGG CC^T[ACGT]AGG
1537 CCTTAGG
CfoI 61 GCGC GCG^C
150 GCGC
DsaI 92 CCATGG C^C[GA][CT]GG
180 CCGCGG
Eco81I 442 CCTGAGG CC^T[ACGT]AGG
1537 CCTTAGG
HapII 572 CCGG C^CGG
1033 CCGG
HhaI 61 GCGC GCG^C
150 GCGC
HinP1I 61 GCGC G^CGC
150 GCGC
HpaII 572 CCGG C^CGG
1033 CCGG
MaeIII 121 GTCAC ^GT[ACGT]AC
575 GTTAC
MspI 572 CCGG C^CGG
1033 CCGG
PflMI 549 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1048 CCAAATGATGG
PpuMI 558 GGGTCCT [GA]G^G[AT]CC[CT]
1082 AGGACCC
SapI 135 GAAGAGC GCTCTTC(1/4)
1484 GAAGAGC
Van91I 549 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1048 CCAAATGATGG
XmnI 478 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1623 GAAGTGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BanII BbvI BfaI BglI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmI Bsp1286I BssKI
BstAPI BstF5I BstNI BstUI Cac8I
Cfr13I DdeI DpnII DraI DraII
DraIII EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HgaI HinfI
HphI ItaI Ksp632I MaeI MaeII
MboI MboII MnlI MseI MslI
MspA1I MvaI MvnI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StuI StyI TfiI Tru9I TseI
Tsp509I TspRI