##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AflIII      
AlwNI       AocI        Aor51HI     ApaI        AscI        
AseI        AsnI        Asp718I     AviII       AvrII       
BalI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BfrI        BlnI        BsaAI       BsgI        
BsiWI       BsmBI       Bsp1407I    BspDI       BspLU11I    
BsrBI       BsrGI       BssHII      BssSI       BstAPI      
BstBI       Bsu36I      ClaI        CpoI        DrdI        
Eco47III    Eco81I      EcoRV       FseI        FspI        
HaeII       HincII      HindII      HpaI        KasI        
KpnI        MfeI        MluI        MluNI       MscI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NotI        NruI        NspV        PacI        PaeR7I      
PflMI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SalI        
SbfI        SexAI       SfoI        SfuI        SmlI        
SnaBI       SpeI        Sse8387I    SspBI       SwaI        
Van91I      XhoI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AccI         2173  GTATAC               GT^[AC][GT]AC
AccIII        164  TCCGGA               T^CCGGA
AcyI          105  GGCGTC               G[GA]^CG[CT]C
AgeI         1772  ACCGGT               A^CCGGT
AhdI         1761  GACATGCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2265  GTGCAC               G^TGCAC
ApaLI        2265  GTGCAC               G^TGCAC
Asp700I      1008  GAATACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1761  GACATGCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2367  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        2465  GGATCC               G^GATCC
BanII         700  GAGCTC               G[GA]GC[CT]^C
BlpI          237  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      237  GCTCAGC              GC^T[ACGT]AGC
BsaBI         502  GATCCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         105  GGCGTC               G[GA]^CG[CT]C
BseAI         164  TCCGGA               T^CCGGA
BsiEI         217  CGGCCG               CG[GA][CT]^CG
BsmI         1482  GCATTC               GAATGC(1/-1)
BspEI         164  TCCGGA               T^CCGGA
BspHI         980  TCATGA               T^CATGA
BsrDI         776  GCAATG               GCAATG(2/0)
Bst1107I     2173  GTATAC               GTA^TAC
BstZ17I      2173  GTATAC               GTA^TAC
CelII         237  GCTCAGC              GC^T[ACGT]AGC
DraIII       1134  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          217  CGGCCG               C^GGCCG
Eam1105I     1761  GACATGCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         217  CGGCCG               C^GGCCG
Eco52I        217  CGGCCG               C^GGCCG
EcoRI        1070  GAATTC               G^AATTC
FauI           96  GCGGG                CCCGC(4/6)
Hin1I         105  GGCGTC               G[GA]^CG[CT]C
HindIII       846  AAGCTT               A^AGCTT
KspI           78  CCGCGG               CCGC^GG
MamI          502  GATCCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          164  TCCGGA               T^CCGGA
MslI          348  CATCAGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI          910  GCTAGC               G^CTAGC
PflFI        2367  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1772  ACCGGT               A^CCGGT
PpuMI          44  GGGTCCC              [GA]G^G[AT]CC[CT]
PstI          895  CTGCAG               CTGCA^G
PvuII         994  CAGCTG               CAG^CTG
RcaI          980  TCATGA               T^CATGA
SacI          700  GAGCTC               GAGCT^C
SacII          78  CCGCGG               CCGC^GG
SapI          702  GCTCTTC              GCTCTTC(1/4)
ScaI         1112  AGTACT               AGT^ACT
SfiI          630  GGCCATGAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI        1771  CACCGGTG             C[GA]^CCGG[CT]G
SmaI           40  CCCGGG               CCC^GGG
SphI         1437  GCATGC               GCATG^C
SspI         2238  AATATT               AAT^ATT
StuI          638  AGGCCT               AGG^CCT
Tth111I      2367  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1378  CCAGAGATCTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI          40  CCCGGG               C^CCGGG
XmaI           40  CCCGGG               C^CCGGG
XmnI         1008  GAATACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AvaI           40  CCCGGG               C^[CT]CG[GA]G
               57  CTCGGG              
BanI         1366  GGCACC               G^G[CT][GA]CC
             2322  GGTGCC              
BbsI          298  GAAGAC               GAAGAC(2/6)
             1420  GAAGAC              
BglII        1221  AGATCT               A^GATCT
             1382  AGATCT              
BpuAI         298  GAAGAC               GAAGAC(2/6)
             1420  GAAGAC              
BsaI          661  GGTCTC               GGTCTC(1/5)
             1738  GGTCTC              
BsaWI         164  TCCGGA               [AT]^CCGG[AT]
             1772  ACCGGT              
BseRI         718  CTCCTC               GAGGAG(10/8)
             1601  GAGGAG              
BsoBI          40  CCCGGG               C^[CT]CG[GA]G
               57  CTCGGG              
BspMI        2279  ACCTGC               ACCTGC(4/8)
              474  GCAGGT              
DraI         2475  TTTAAA               TTT^AAA
             2495  TTTAAA              
DraII          44  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             1719  GGGGCCT             
EaeI          217  CGGCCG               [CT]^GGCC[GA]
             1691  TGGCCG              
EcoO109I       44  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             1719  GGGGCCT             
EcoT22I       157  ATGCAT               ATGCA^T
              712  ATGCAT              
HgaI          106  GCGTC                GACGC(5/10)
             1696  GCGTC               
MaeII        1732  ACGT                 A^CGT
             2456  ACGT                
NsiI          157  ATGCAT               ATGCA^T
              712  ATGCAT              
NspI         1437  GCATGC               [GA]CATG^[CT]
             1762  ACATGC              
SfcI          895  CTGCAG               C^T[GA][CT]AG
             1371  CTACAG              
XbaI         2092  TCTAGA               T^CTAGA
             2428  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AspHI       AvaII       BbvI        
BclI        BcnI        BfaI        BglI        BmyI        
BpmI        BsaJI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrFI       BsrI        
BssKI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DsaI        
EarI        EcoNI       EcoO65I     EcoRII      EcoT14I     
FbaI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII