##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BcgI BglI BlnI BlpI BpmI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsoBI BspDI
BspEI BspHI BspLU11I BsrFI BssHII
BssSI BstAPI BstEII BstPI Bsu36I
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I FseI FspI HaeII
HgaI Hin1I KasI KpnI KspI
MamI MluI MluNI MroI MscI
NaeI NarI NdeI NgoMIV NotI
NruI PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AlwI 3125 GATCC GGATC(4/5)
AlwNI 928 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 2680 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2904 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 3223 GGCACC G^G[CT][GA]CC
BbsI 641 GAAGAC GAAGAC(2/6)
BbvCI 2999 CCTCAGC CCTCAGC(-5/-2)
BclI 1526 TGATCA T^GATCA
BcnI 4 CCGGG CC^[GC]GG
BglII 891 AGATCT A^GATCT
BmyI 1362 GTGCCC G[AGT]GC[ACT]^C
BpuAI 641 GAAGAC GAAGAC(2/6)
BseRI 1433 CTCCTC GAGGAG(10/8)
BsmBI 3071 GAGACG CGTCTC(1/5)
BsmI 3399 GCATTC GAATGC(1/-1)
Bsp1286I 1362 GTGCCC G[AGT]GC[ACT]^C
BspMI 2975 GCAGGT ACCTGC(4/8)
BsrBI 177 GAGCGG CCGCTC(-3/-3)
BsrDI 241 GCAATG GCAATG(2/0)
Bst1107I 1938 GTATAC GTA^TAC
BstBI 2600 TTCGAA TT^CGAA
BstXI 3145 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 891 AGATCT [GA]^GATC[CT]
BstZ17I 1938 GTATAC GTA^TAC
DsaI 172 CCATGG C^C[GA][CT]GG
EcoRI 2351 GAATTC G^AATTC
EcoRV 260 GATATC GAT^ATC
EcoT22I 1721 ATGCAT ATGCA^T
FauI 17 CCCGC CCCGC(4/6)
FbaI 1526 TGATCA T^GATCA
HapII 4 CCGG C^CGG
HpaI 1342 GTTAAC GTT^AAC
HpaII 4 CCGG C^CGG
MfeI 1835 CAATTG C^AATTG
MflI 891 AGATCT [GA]^GATC[CT]
MspI 4 CCGG C^CGG
MunI 1835 CAATTG C^AATTG
NciI 4 CCGGG CC^[GC]GG
NcoI 172 CCATGG C^CATGG
NheI 527 GCTAGC G^CTAGC
NsiI 1721 ATGCAT ATGCA^T
NspV 2600 TTCGAA TT^CGAA
PflFI 2904 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3028 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1828 CTGCAG CTGCA^G
PvuII 2225 CAGCTG CAG^CTG
SapI 2794 GCTCTTC GCTCTTC(1/4)
SfuI 2600 TTCGAA TT^CGAA
SphI 2245 GCATGC GCATG^C
StuI 2866 AGGCCT AGG^CCT
Tth111I 2904 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2861 CCAAGAGGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 891 AGATCT [GA]^GATC[CT]
XmnI 2680 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflII 1503 CTTAAG C^TTAAG
3569 CTTAAG
AseI 2128 ATTAAT AT^TAAT
2143 ATTAAT
AsnI 2128 ATTAAT AT^TAAT
2143 ATTAAT
AvaII 1920 GGTCC G^G[AT]CC
3020 GGACC
BciVI 226 GGATAC GTATCC(6/5)
1705 GGATAC
BfrI 1503 CTTAAG C^TTAAG
3569 CTTAAG
BsaAI 1672 CACGTA [CT]AC^GT[GA]
1685 CACGTA
BsgI 389 CTGCAC GTGCAG(16/14)
2935 GTGCAG
BsmFI 3019 GGGAC GGGAC(10/14)
1792 GTCCC
CfoI 11 GCGC GCG^C
20 GCGC
EcoT14I 172 CCATGG C^C[AT][AT]GG
3135 CCTTGG
HhaI 11 GCGC GCG^C
20 GCGC
HinP1I 11 GCGC G^CGC
20 GCGC
HindIII 953 AAGCTT A^AGCTT
3566 AAGCTT
MspA1I 48 CCGCTG C[AC]G^C[GT]G
2225 CAGCTG
PleI 1254 GACTC GAGTC(4/5)
2908 GAGTC
PshBI 2128 ATTAAT AT^TAAT
2143 ATTAAT
SfaNI 293 GATGC GCATC(5/9)
913 GCATC
StyI 172 CCATGG C^C[AT][AT]GG
3135 CCTTGG
SwaI 1100 ATTTAAAT ATTT^AAAT
3523 ATTTAAAT
XbaI 1145 TCTAGA T^CTAGA
1946 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI ApoI BbvI BfaI BsaI
BsaJI BsiYI BslI BsmAI Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstUI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRII Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI RsaI Sau3AI Sau96I
ScrFI SfcI SmlI SspBI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I