##################################
# NO CUT ENZYMES (   59 enzymes) #
##################################

Acc65I      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        Asp718I     AspEI       
AspI        AviII       AvrII       BglII       BlnI        
BsiWI       BspDI       BssHII      Bst1107I    BstBI       
BstZ17I     Bsu36I      ClaI        CpoI        DraIII      
Eam1105I    Eco47III    Eco81I      FspI        HpaI        
KpnI        KspI        MluI        NdeI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshAI       PvuI        RsrII       
SacII       SapI        SbfI        SfiI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    StuI        
SwaI        Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII        1753  GACGTC               GACGT^C
AccI         3791  GTCGAC               GT^[AC][GT]AC
AccIII       2319  TCCGGA               T^CCGGA
BamHI          50  GGATCC               G^GATCC
BanI         1497  GGCGCC               G^G[CT][GA]CC
BbeI         1497  GGCGCC               GGCGC^C
BbrPI        2803  CACGTG               CAC^GTG
BbsI         2911  GTCTTC               GAAGAC(2/6)
BcgI          882  CGAGGAAGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2074  GTATCC               GTATCC(6/5)
BlpI         2047  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2047  GCTGAGC              GC^T[ACGT]AGC
BpuAI        2911  GTCTTC               GAAGAC(2/6)
BseAI        2319  TCCGGA               T^CCGGA
BsmI         3389  GAATGC               GAATGC(1/-1)
Bsp1407I     4476  TGTACA               T^GTACA
BspEI        2319  TCCGGA               T^CCGGA
BspLU11I      198  ACATGT               A^CATGT
BspMI        4373  GCAGGT               ACCTGC(4/8)
BsrBI        1491  GAGCGG               CCGCTC(-3/-3)
BsrGI        4476  TGTACA               T^GTACA
BssSI        1513  CACGAG               CACGAG(-5/-1)
BstAPI       2659  GCAGAAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2423  GGTCACC              G^GT[ACGT]ACC
BstPI        2423  GGTCACC              G^GT[ACGT]ACC
CelII        2047  GCTGAGC              GC^T[ACGT]AGC
DrdI          712  GACCTGGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1257  CCTGGCAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      2423  GGTCACC              G^GT[ACGT]ACC
EcoRI         282  GAATTC               G^AATTC
EcoT22I       937  ATGCAT               ATGCA^T
FseI         1456  GGCCGGCC             GGCCGG^CC
KasI         1497  GGCGCC               G^GCGCC
MfeI         2951  CAATTG               C^AATTG
MroI         2319  TCCGGA               T^CCGGA
MunI         2951  CAATTG               C^AATTG
NaeI         1457  GCCGGC               GCC^GGC
NarI         1497  GGCGCC               GG^CGCC
NgoMIV       1457  GCCGGC               G^CCGGC
NsiI          937  ATGCAT               ATGCA^T
PmaCI        2803  CACGTG               CAC^GTG
PmlI         2803  CACGTG               CAC^GTG
PstI         2612  CTGCAG               CTGCA^G
SacI          442  GAGCTC               GAGCT^C
SalI         3791  GTCGAC               G^TCGAC
SexAI        1353  ACCTGGT              A^CC[AT]GGT
SfoI         1497  GGCGCC               GGC^GCC
SphI         2013  GCATGC               GCATG^C
SspBI        4476  TGTACA               T^GTACA
SspI         3994  AATATT               AAT^ATT
XcmI         1778  CCAAATACACCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AclI         3223  AACGTT               AA^CGTT
             4198  AACGTT              
AflII        1024  CTTAAG               C^TTAAG
             2935  CTTAAG              
AflIII        198  ACATGT               A^C[GA][CT]GT
             2773  ACGTGT              
AseI         3371  ATTAAT               AT^TAAT
             4426  ATTAAT              
AsnI         3371  ATTAAT               AT^TAAT
             4426  ATTAAT              
BalI         1472  TGGCCA               TGG^CCA
             3748  TGGCCA              
BbvCI        1894  GCTGAGG              CCTCAGC(-5/-2)
             4417  GCTGAGG             
BclI         2444  TGATCA               T^GATCA
             4262  TGATCA              
BfrI         1024  CTTAAG               C^TTAAG
             2935  CTTAAG              
BglI          892  GCCTTCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1383  GCCCTTGGGGC         
BpmI          586  CTCCAG               CTGGAG(16/14)
             2705  CTCCAG              
BsaAI        2803  CACGTG               [CT]AC^GT[GA]
             2922  CACGTA              
BsaBI        1366  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3879  GATTTTGATC          
BsaI         1252  GAGACC               GGTCTC(1/5)
             2283  GAGACC              
BsaWI        2319  TCCGGA               [AT]^CCGG[AT]
             3042  TCCGGT              
BsmBI        1755  CGTCTC               CGTCTC(1/5)
             2515  GAGACG              
BspHI         278  TCATGA               T^CATGA
              990  TCATGA              
BsrDI        2816  CATTGC               GCAATG(2/0)
             4090  CATTGC              
BsrFI        1457  GCCGGC               [GA]^CCGG[CT]
             2652  ACCGGC              
BstXI        2130  CCAGCTAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3250  CCAGATCCATGG        
Cfr10I       1457  GCCGGC               [GA]^CCGG[CT]
             2652  ACCGGC              
EarI          913  CTCTTC               CTCTTC(1/4)
             3351  GAAGAG              
FbaI         2444  TGATCA               T^GATCA
             4262  TGATCA              
Ksp632I       913  CTCTTC               CTCTTC(1/4)
             3351  GAAGAG              
MamI         1366  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3879  GATTTTGATC          
MluNI        1472  TGGCCA               TGG^CCA
             3748  TGGCCA              
MscI         1472  TGGCCA               TGG^CCA
             3748  TGGCCA              
NheI         2154  GCTAGC               G^CTAGC
             2545  GCTAGC              
PshBI        3371  ATTAAT               AT^TAAT
             4426  ATTAAT              
Psp1406I     3223  AACGTT               AA^CGTT
             4198  AACGTT              
PvuII        1679  CAGCTG               CAG^CTG
             3409  CAGCTG              
RcaI          278  TCATGA               T^CATGA
              990  TCATGA              
ScaI         3115  AGTACT               AGT^ACT
             3649  AGTACT              
SmaI           31  CCCGGG               CCC^GGG
              140  CCCGGG              
XmaCI          31  CCCGGG               C^CCGGG
              140  CCCGGG              
XmaI           31  CCCGGG               C^CCGGG
              140  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        ApoI        
Asp700I     AspHI       AvaI        AvaII       BanII       
BbvI        BcnI        BfaI        BmyI        BsaHI       
BsaJI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EaeI        EagI        EclXI       Eco52I      EcoO109I    
EcoRII      EcoRV       EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HinfI       HpaII       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       XmnI