##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccII AclI AcyI AflIII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI AviII
AvrII BbeI BbrPI BciVI BglI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsiEI BsiWI BspLU11I BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
CpoI DraIII EagI EclXI Eco47III
Eco52I Eco81I EcoRI EcoT22I FseI
FspI HaeII HhaI Hin1I HinP1I
HpaI KasI KspI MluI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 2231 GGTACC G^GTACC
AccIII 3788 TCCGGA T^CCGGA
AflII 27 CTTAAG C^TTAAG
AhdI 1536 GACAGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 2231 GGTACC G^GTACC
AspEI 1536 GACAGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2714 GAGCTC G[AT]GC[AT]^C
BcgI 1953 GCAGCGGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3428 TGATCA T^GATCA
BcnI 3821 CCCGG CC^[GC]GG
BfrI 27 CTTAAG C^TTAAG
BglII 263 AGATCT A^GATCT
BsaBI 3355 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3788 TCCGGA T^CCGGA
BseRI 1835 GAGGAG GAGGAG(10/8)
BsiHKAI 2714 GAGCTC G[AT]GC[AT]^C
BspDI 1044 ATCGAT AT^CGAT
BspEI 3788 TCCGGA T^CCGGA
BspHI 3431 TCATGA T^CATGA
BsrDI 2729 GCAATG GCAATG(2/0)
ClaI 1044 ATCGAT AT^CGAT
DraI 804 TTTAAA TTT^AAA
Eam1105I 1536 GACAGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 3428 TGATCA T^GATCA
HgaI 1542 GCGTC GACGC(5/10)
HindIII 2941 AAGCTT A^AGCTT
KpnI 2231 GGTACC GGTAC^C
MamI 3355 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1784 CAATTG C^AATTG
MroI 3788 TCCGGA T^CCGGA
MunI 1784 CAATTG C^AATTG
NciI 3821 CCCGG CC^[GC]GG
NcoI 3707 CCATGG C^CATGG
NdeI 3312 CATATG CA^TATG
PaeR7I 3778 CTCGAG C^TCGAG
PshAI 403 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3431 TCATGA T^CATGA
SacI 2714 GAGCTC GAGCT^C
SalI 1544 GTCGAC G^TCGAC
ScaI 897 AGTACT AGT^ACT
SphI 2042 GCATGC GCATG^C
StuI 119 AGGCCT AGG^CCT
XbaI 4254 TCTAGA T^CTAGA
XcmI 465 CCAAGAAACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3778 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 1544 GTCGAC GT^[AC][GT]AC
4115 GTCTAC
AlwNI 866 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
3836 CAGAGACTG
Asp700I 215 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2253 GAAGCTCTTC
AvaI 2407 CCCGAG C^[CT]CG[GA]G
3778 CTCGAG
BanI 373 GGCACC G^G[CT][GA]CC
2231 GGTACC
BsaI 3683 GAGACC GGTCTC(1/5)
1958 GGTCTC
BsaWI 341 TCCGGT [AT]^CCGG[AT]
3788 TCCGGA
BsmBI 3732 CGTCTC CGTCTC(1/5)
3451 GAGACG
BsoBI 2407 CCCGAG C^[CT]CG[GA]G
3778 CTCGAG
Bsp1407I 164 TGTACA T^GTACA
4170 TGTACA
BsrBI 2307 CCGCTC CCGCTC(-3/-3)
2647 GAGCGG
BsrGI 164 TGTACA T^GTACA
4170 TGTACA
BstEII 940 GGTGACC G^GT[ACGT]ACC
1516 GGTAACC
BstPI 940 GGTGACC G^GT[ACGT]ACC
1516 GGTAACC
EcoNI 997 CCTGAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1433 CCTGTGGTAGG
EcoO65I 940 GGTGACC G^GT[ACGT]ACC
1516 GGTAACC
HincII 1544 GTCGAC GT[CT]^[GA]AC
3935 GTCAAC
HindII 1544 GTCGAC GT[CT]^[GA]AC
3935 GTCAAC
MaeII 3553 ACGT A^CGT
3731 ACGT
NspI 2042 GCATGC [GA]CATG^[CT]
3582 GCATGT
PflMI 116 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3579 CCAGCATGTGG
PvuII 1873 CAGCTG CAG^CTG
2797 CAGCTG
SapI 2256 GCTCTTC GCTCTTC(1/4)
3956 GCTCTTC
SexAI 1512 ACCTGGT A^CC[AT]GGT
4020 ACCTGGT
SspBI 164 TGTACA T^GTACA
4170 TGTACA
SspI 1139 AATATT AAT^ATT
2152 AATATT
Van91I 116 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3579 CCAGCATGTGG
XmnI 215 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2253 GAAGCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspI AvaII BalI BamHI
BanII BbsI BbvCI BbvI BfaI
BmyI BpmI BpuAI BsaJI BsgI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI EcoO109I EcoRII EcoRV
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PflFI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XhoII