##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AccIII AgeI AscI AseI
AsnI AspI BglII BsaBI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BsrGI Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EcoRI EcoRV
HindIII HpaI MamI MfeI MroI
MunI NdeI NheI NotI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI PvuI RcaI RsrII
SalI ScaI SexAI SfuI SnaBI
SpeI SspBI SspI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccI 1746 GTCTAC GT^[AC][GT]AC
AcsI 2172 GAATTT [GA]^AATT[CT]
AflII 3823 CTTAAG C^TTAAG
AhdI 1262 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1425 GTGCAC G^TGCAC
Aor51HI 2836 AGCGCT AGC^GCT
ApaLI 1425 GTGCAC G^TGCAC
ApoI 2172 GAATTT [GA]^AATT[CT]
Asp700I 2427 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1262 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 82 CCTAGG C^CTAGG
BamHI 533 GGATCC G^GATCC
BcgI 2294 GCAGCCCCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3823 CTTAAG C^TTAAG
BlnI 82 CCTAGG C^CTAGG
BsaWI 1330 TCCGGT [AT]^CCGG[AT]
BsmBI 4235 GAGACG CGTCTC(1/5)
BsmI 3392 GAATGC GAATGC(1/-1)
BspLU11I 2155 ACATGT A^CATGT
BssSI 2760 CTCGTG CACGAG(-5/-1)
Eam1105I 1262 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2836 AGCGCT AGC^GCT
EcoT22I 584 ATGCAT ATGCA^T
NruI 3265 TCGCGA TCG^CGA
NsiI 584 ATGCAT ATGCA^T
SmaI 1849 CCCGGG CCC^GGG
TaqI 5217 TCGA T^CGA
TthHB8I 5217 TCGA T^CGA
XmaCI 1849 CCCGGG C^CCGGG
XmaI 1849 CCCGGG C^CCGGG
XmnI 2427 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 1333 GGTACC G^GTACC
4255 GGTACC
AclI 2497 AACGTT AA^CGTT
5300 AACGTT
ApaI 1845 GGGCCC GGGCC^C
5109 GGGCCC
Asp718I 1333 GGTACC G^GTACC
4255 GGTACC
AviII 766 TGCGCA TGC^GCA
4686 TGCGCA
BclI 862 TGATCA T^GATCA
1834 TGATCA
BsaI 87 GGTCTC GGTCTC(1/5)
3800 GGTCTC
BstEII 2205 GGTCACC G^GT[ACGT]ACC
3291 GGTCACC
BstPI 2205 GGTCACC G^GT[ACGT]ACC
3291 GGTCACC
EagI 3029 CGGCCG C^GGCCG
3255 CGGCCG
EclXI 3029 CGGCCG C^GGCCG
3255 CGGCCG
Eco52I 3029 CGGCCG C^GGCCG
3255 CGGCCG
EcoO65I 2205 GGTCACC G^GT[ACGT]ACC
3291 GGTCACC
FbaI 862 TGATCA T^GATCA
1834 TGATCA
FseI 2362 GGCCGGCC GGCCGG^CC
3256 GGCCGGCC
FspI 766 TGCGCA TGC^GCA
4686 TGCGCA
HincII 2826 GTTGAC GT[CT]^[GA]AC
3078 GTTGAC
HindII 2826 GTTGAC GT[CT]^[GA]AC
3078 GTTGAC
KpnI 1333 GGTACC GGTAC^C
4255 GGTACC
KspI 2617 CCGCGG CCGC^GG
5155 CCGCGG
MluI 3074 ACGCGT A^CGCGT
5172 ACGCGT
MseI 3824 TTAA T^TAA
4033 TTAA
NcoI 1363 CCATGG C^CATGG
3549 CCATGG
Psp1406I 2497 AACGTT AA^CGTT
5300 AACGTT
SacII 2617 CCGCGG CCGC^GG
5155 CCGCGG
SgrAI 2881 CGCCGGCG C[GA]^CCGG[CT]G
3113 CGCCGGCG
Tru9I 3824 TTAA T^TAA
4033 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbrPI BbsI BbvCI BbvI
BciVI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssHII BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SfiI SfoI
SmlI SphI Sse8387I StuI StyI
TfiI TseI Tsp45I Tsp509I TspRI
Van91I XcmI XhoII