##################################
# NO CUT ENZYMES (   80 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        Asp718I     AspEI       AviII       BamHI       
BbeI        BbrPI       BglI        BsaAI       BsaBI       
BsaHI       BsiWI       BssHII      BssSI       Bst1107I    
BstUI       BstZ17I     CpoI        DraIII      EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MunI        MvnI        
NarI        NcoI        NdeI        NheI        NotI        
NruI        PacI        PaeR7I      PmaCI       PmeI        
PmlI        PshAI       Psp1406I    RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SnaBI       
SphI        Sse8387I    SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI         3551  GTCTAC               GT^[AC][GT]AC
AccIII        717  TCCGGA               T^CCGGA
AgeI         1817  ACCGGT               A^CCGGT
AocI         3689  CCTTAGG              CC^T[ACGT]AGG
AspI         4058  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         3587  TGGCCA               TGG^CCA
BanII        1110  GAGCCC               G[GA]GC[CT]^C
BcgI          806  GCAGTGCGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         714  GTATCC               GTATCC(6/5)
BlpI         1698  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     1698  GCTAAGC              GC^T[ACGT]AGC
BseAI         717  TCCGGA               T^CCGGA
BsiEI         812  CGATCG               CG[GA][CT]^CG
BspEI         717  TCCGGA               T^CCGGA
BsrBI         621  GAGCGG               CCGCTC(-3/-3)
BsrDI        1708  CATTGC               GCAATG(2/0)
BstBI        2014  TTCGAA               TT^CGAA
BstEII        450  GGTGACC              G^GT[ACGT]ACC
BstPI         450  GGTGACC              G^GT[ACGT]ACC
BstXI        4278  CCAAAGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       3689  CCTTAGG              CC^T[ACGT]AGG
CelII        1698  GCTAAGC              GC^T[ACGT]AGC
DrdI         4058  GACTCTGTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       3689  CCTTAGG              CC^T[ACGT]AGG
EcoO65I       450  GGTGACC              G^GT[ACGT]ACC
EcoRI        2868  GAATTC               G^AATTC
EcoRV        5345  GATATC               GAT^ATC
HaeII         257  AGCGCC               [GA]GCGC^[CT]
MluNI        3587  TGGCCA               TGG^CCA
MroI          717  TCCGGA               T^CCGGA
MscI         3587  TGGCCA               TGG^CCA
MspA1I       1123  CAGCTG               C[AC]G^C[GT]G
NaeI          221  GCCGGC               GCC^GGC
NgoMIV        221  GCCGGC               G^CCGGC
NspV         2014  TTCGAA               TT^CGAA
PflFI        4058  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1817  ACCGGT               A^CCGGT
PvuI          812  CGATCG               CGAT^CG
PvuII        1123  CAGCTG               CAG^CTG
SfuI         2014  TTCGAA               TT^CGAA
SmaI          183  CCCGGG               CCC^GGG
SpeI         2255  ACTAGT               A^CTAGT
StuI         3054  AGGCCT               AGG^CCT
Tth111I      4058  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI         183  CCCGGG               C^CCGGG
XmaI          183  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AflII        1083  CTTAAG               C^TTAAG
             3213  CTTAAG              
AspHI        1597  GAGCAC               G[AT]GC[AT]^C
             3355  GTGCTC              
AvaI          183  CCCGGG               C^[CT]CG[GA]G
             4145  CCCGAG              
AvrII        3051  CCTAGG               C^CTAGG
             5531  CCTAGG              
BanI          191  GGCACC               G^G[CT][GA]CC
             4165  GGTGCC              
BbvCI        4136  CCTCAGC              CCTCAGC(-5/-2)
             4268  CCTCAGC             
BclI         1900  TGATCA               T^GATCA
             3146  TGATCA              
BfrI         1083  CTTAAG               C^TTAAG
             3213  CTTAAG              
BglII        2019  AGATCT               A^GATCT
             3574  AGATCT              
BlnI         3051  CCTAGG               C^CTAGG
             5531  CCTAGG              
BseRI          46  GAGGAG               GAGGAG(10/8)
              284  GAGGAG              
BsiHKAI      1597  GAGCAC               G[AT]GC[AT]^C
             3355  GTGCTC              
BsmBI         158  GAGACG               CGTCTC(1/5)
             4208  GAGACG              
BsmI         3536  GAATGC               GAATGC(1/-1)
             5244  GCATTC              
BsoBI         183  CCCGGG               C^[CT]CG[GA]G
             4145  CCCGAG              
BspDI        1416  ATCGAT               AT^CGAT
             1833  ATCGAT              
BspHI        3623  TCATGA               T^CATGA
             5009  TCATGA              
BsrFI         221  GCCGGC               [GA]^CCGG[CT]
             1817  ACCGGT              
BstAPI        758  GCAGCATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4410  GCAAGTTGTGC         
CfoI          258  GCGC                 GCG^C
             4087  GCGC                
Cfr10I        221  GCCGGC               [GA]^CCGG[CT]
             1817  ACCGGT              
ClaI         1416  ATCGAT               AT^CGAT
             1833  ATCGAT              
EcoT22I      1423  ATGCAT               ATGCA^T
             5457  ATGCAT              
FbaI         1900  TGATCA               T^GATCA
             3146  TGATCA              
HhaI          258  GCGC                 GCG^C
             4087  GCGC                
HinP1I        258  GCGC                 G^CGC
             4087  GCGC                
NsiI         1423  ATGCAT               ATGCA^T
             5457  ATGCAT              
PflMI         900  CCAGAATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4107  CCACTTCCTGG         
PstI         1396  CTGCAG               CTGCA^G
             1906  CTGCAG              
RcaI         3623  TCATGA               T^CATGA
             5009  TCATGA              
Van91I        900  CCAGAATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4107  CCACTTCCTGG         
XcmI         2497  CCAAAGAGACTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4782  CCAATATAGATTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        AseI        AsnI        
Asp700I     AvaII       BbsI        BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaI        
BsaJI       BsaWI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    Bsp1407I    BspLU11I    
BspMI       BsrGI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HindIII     
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PpuMI       PshBI       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspBI       SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmnI