##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
Acc65I AccII AclI AflII AflIII
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI BalI BbeI BbrPI
BfrI BsaAI BsaBI BsiEI BsiWI
BsmBI BsoBI BspDI BspLU11I BssHII
BstAPI BstUI ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoRI EcoRV EcoT22I HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MscI MunI MvnI
NarI NcoI NdeI NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI Tth111I XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 1970 GACGTC GACGT^C
AccIII 1584 TCCGGA T^CCGGA
AcyI 1970 GACGTC G[GA]^CG[CT]C
AlwNI 625 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AviII 2486 TGCGCA TGC^GCA
BbsI 775 GAAGAC GAAGAC(2/6)
BbvCI 2999 CCTCAGC CCTCAGC(-5/-2)
BcgI 1264 GCAGGAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1600 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1600 GCTCAGC GC^T[ACGT]AGC
BpuAI 775 GAAGAC GAAGAC(2/6)
BsaHI 1970 GACGTC G[GA]^CG[CT]C
BseAI 1584 TCCGGA T^CCGGA
BsgI 170 GTGCAG GTGCAG(16/14)
BsmI 2508 GCATTC GAATGC(1/-1)
Bsp1407I 1116 TGTACA T^GTACA
BspEI 1584 TCCGGA T^CCGGA
BspHI 3019 TCATGA T^CATGA
BsrDI 2226 CATTGC GCAATG(2/0)
BsrFI 79 GCCGGC [GA]^CCGG[CT]
BsrGI 1116 TGTACA T^GTACA
Bst1107I 3460 GTATAC GTA^TAC
BstBI 2621 TTCGAA TT^CGAA
BstZ17I 3460 GTATAC GTA^TAC
CelII 1600 GCTCAGC GC^T[ACGT]AGC
Cfr10I 79 GCCGGC [GA]^CCGG[CT]
DraI 3429 TTTAAA TTT^AAA
FseI 78 GGCCGGCC GGCCGG^CC
FspI 2486 TGCGCA TGC^GCA
HgaI 2107 GACGC GACGC(5/10)
Hin1I 1970 GACGTC G[GA]^CG[CT]C
HincII 2410 GTCAAC GT[CT]^[GA]AC
HindII 2410 GTCAAC GT[CT]^[GA]AC
MroI 1584 TCCGGA T^CCGGA
NaeI 79 GCCGGC GCC^GGC
NgoMIV 79 GCCGGC G^CCGGC
NspI 2489 GCATGC [GA]CATG^[CT]
NspV 2621 TTCGAA TT^CGAA
PflMI 3378 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 2536 CAGCTG CAG^CTG
RcaI 3019 TCATGA T^CATGA
SapI 637 GAAGAGC GCTCTTC(1/4)
SfuI 2621 TTCGAA TT^CGAA
SphI 2489 GCATGC GCATG^C
SspBI 1116 TGTACA T^GTACA
SspI 3200 AATATT AAT^ATT
Van91I 3378 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3568 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 1954 AAATTC [GA]^AATT[CT]
3472 AAATTT
AocI 553 CCTGAGG CC^T[ACGT]AGG
676 CCTGAGG
Aor51HI 1193 AGCGCT AGC^GCT
2000 AGCGCT
ApoI 1954 AAATTC [GA]^AATT[CT]
3472 AAATTT
AvrII 319 CCTAGG C^CTAGG
3042 CCTAGG
BamHI 2176 GGATCC G^GATCC
3194 GGATCC
BciVI 42 GGATAC GTATCC(6/5)
1817 GGATAC
BcnI 1520 CCCGG CC^[GC]GG
2216 CCGGG
BlnI 319 CCTAGG C^CTAGG
3042 CCTAGG
BsaWI 1584 TCCGGA [AT]^CCGG[AT]
1943 ACCGGA
BsrBI 1073 CCGCTC CCGCTC(-3/-3)
1225 CCGCTC
BssSI 875 CACGAG CACGAG(-5/-1)
3251 CACGAG
BstEII 2113 GGTTACC G^GT[ACGT]ACC
3232 GGTAACC
BstPI 2113 GGTTACC G^GT[ACGT]ACC
3232 GGTAACC
BstXI 1189 CCAGAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2788 CCAGATCCTTGG
Bsu36I 553 CCTGAGG CC^T[ACGT]AGG
676 CCTGAGG
EaeI 336 TGGCCG [CT]^GGCC[GA]
523 CGGCCA
Eco47III 1193 AGCGCT AGC^GCT
2000 AGCGCT
Eco81I 553 CCTGAGG CC^T[ACGT]AGG
676 CCTGAGG
EcoO65I 2113 GGTTACC G^GT[ACGT]ACC
3232 GGTAACC
HaeII 1193 AGCGCT [GA]GCGC^[CT]
2000 AGCGCT
MspA1I 1813 CAGCGG C[AC]G^C[GT]G
2536 CAGCTG
NciI 1520 CCCGG CC^[GC]GG
2216 CCGGG
ScaI 1560 AGTACT AGT^ACT
3291 AGTACT
XcmI 1410 CCACTTTGTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2628 CCATTTTGTCCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
ApaI AspHI AvaII BanI BanII
BbvI BclI BfaI BglI BglII
BmyI BpmI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PpuMI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII