##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AclI AcyI AflIII AgeI
AhdI Aor51HI AscI AspEI AviII
BbeI BcnI BlpI Bpu1102I BsaHI
BsiEI BsiWI BsmBI BspDI BspHI
BspLU11I BsrFI Bst1107I BstBI BstZ17I
CelII Cfr10I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV FseI FspI HgaI
Hin1I HpaI KasI KspI MfeI
MluI MunI NaeI NarI NciI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SwaI XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 1399 GGTACC G^GTACC
AccII 2503 CGCG CG^CG
AccIII 71 TCCGGA T^CCGGA
AflII 794 CTTAAG C^TTAAG
Alw44I 2494 GTGCAC G^TGCAC
AlwNI 1281 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 2494 GTGCAC G^TGCAC
AseI 3410 ATTAAT AT^TAAT
AsnI 3410 ATTAAT AT^TAAT
Asp718I 1399 GGTACC G^GTACC
AspI 2160 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1720 CCCGAG C^[CT]CG[GA]G
AvrII 3952 CCTAGG C^CTAGG
BamHI 167 GGATCC G^GATCC
BanI 1399 GGTACC G^G[CT][GA]CC
BbrPI 2497 CACGTG CAC^GTG
BcgI 4517 CGAAGCTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 794 CTTAAG C^TTAAG
BglI 2352 GCCTTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3952 CCTAGG C^CTAGG
BsaAI 2497 CACGTG [CT]AC^GT[GA]
BsaBI 1179 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 656 GAGACC GGTCTC(1/5)
BseAI 71 TCCGGA T^CCGGA
BsoBI 1720 CCCGAG C^[CT]CG[GA]G
Bsp1407I 3474 TGTACA T^GTACA
BspEI 71 TCCGGA T^CCGGA
BsrBI 1395 GAGCGG CCGCTC(-3/-3)
BsrGI 3474 TGTACA T^GTACA
BssHII 2502 GCGCGC G^CGCGC
BssSI 562 CACGAG CACGAG(-5/-1)
BstAPI 3440 GCAGTATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2503 CGCG CG^CG
BstXI 3849 CCACACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 2337 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3967 GACACCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 4461 ATGCAT ATGCA^T
HaeII 3640 AGCGCC [GA]GCGC^[CT]
HindIII 4519 AAGCTT A^AGCTT
KpnI 1399 GGTACC GGTAC^C
MamI 1179 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 71 TCCGGA T^CCGGA
MvnI 2503 CGCG CG^CG
NcoI 2018 CCATGG C^CATGG
NsiI 4461 ATGCAT ATGCA^T
PflFI 2160 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2497 CACGTG CAC^GTG
PmlI 2497 CACGTG CAC^GTG
PshBI 3410 ATTAAT AT^TAAT
SexAI 3596 ACCAGGT A^CC[AT]GGT
SpeI 3216 ACTAGT A^CTAGT
SspBI 3474 TGTACA T^GTACA
StuI 4675 AGGCCT AGG^CCT
Tth111I 2160 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 949 GTCTAC GT^[AC][GT]AC
1771 GTCTAC
ApaI 3492 GGGCCC GGGCC^C
4495 GGGCCC
Asp700I 1293 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2035 GAAAAGATTC
BalI 2746 TGGCCA TGG^CCA
3709 TGGCCA
BciVI 1178 GGATAC GTATCC(6/5)
1087 GTATCC
BglII 1250 AGATCT A^GATCT
4311 AGATCT
BpmI 1131 CTGGAG CTGGAG(16/14)
1272 CTGGAG
BsaWI 71 TCCGGA [AT]^CCGG[AT]
1710 ACCGGA
BsmI 2632 GAATGC GAATGC(1/-1)
1221 GCATTC
BstEII 3039 GGTCACC G^GT[ACGT]ACC
4534 GGTAACC
BstPI 3039 GGTCACC G^GT[ACGT]ACC
4534 GGTAACC
DsaI 1755 CCGTGG C^C[GA][CT]GG
2018 CCATGG
EcoO65I 3039 GGTCACC G^GT[ACGT]ACC
4534 GGTAACC
FauI 2375 CCCGC CCCGC(4/6)
2470 CCCGC
HapII 72 CCGG C^CGG
1711 CCGG
HincII 309 GTCAAC GT[CT]^[GA]AC
3106 GTTGAC
HindII 309 GTCAAC GT[CT]^[GA]AC
3106 GTTGAC
HpaII 72 CCGG C^CGG
1711 CCGG
MluNI 2746 TGGCCA TGG^CCA
3709 TGGCCA
MscI 2746 TGGCCA TGG^CCA
3709 TGGCCA
MspI 72 CCGG C^CGG
1711 CCGG
PstI 804 CTGCAG CTGCA^G
3917 CTGCAG
XmnI 1293 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2035 GAAAAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AspHI AvaII BanII
BbsI BbvCI BbvI BclI BfaI
BmyI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII