##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AclI AcsI AflII AgeI AhdI
AocI Aor51HI ApoI AscI AspEI
AvrII BcgI BciVI BfrI BlnI
BsaI BsmI BspDI BspLU11I Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI Eam1105I Eco47III Eco81I EcoRI
EcoRV EcoT22I FseI HpaI MfeI
MluI MunI NdeI NheI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI Psp1406I PvuI RsrII SalI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 989 GACGTC GACGT^C
Acc65I 3048 GGTACC G^GTACC
AccIII 1669 TCCGGA T^CCGGA
AflIII 1638 ACACGT A^C[GA][CT]GT
AseI 791 ATTAAT AT^TAAT
AsnI 791 ATTAAT AT^TAAT
Asp718I 3048 GGTACC G^GTACC
BamHI 3087 GGATCC G^GATCC
BbeI 4252 GGCGCC GGCGC^C
BfaI 2867 CTAG C^TAG
BsaBI 2998 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1669 TCCGGA T^CCGGA
BsiWI 3096 CGTACG C^GTACG
Bsp1407I 2475 TGTACA T^GTACA
BspEI 1669 TCCGGA T^CCGGA
BspHI 1032 TCATGA T^CATGA
BsrDI 2466 GCAATG GCAATG(2/0)
BsrGI 2475 TGTACA T^GTACA
DrdI 314 GACGACAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3825 CCTTGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 525 AAGCTT A^AGCTT
KasI 4252 GGCGCC G^GCGCC
KpnI 3048 GGTACC GGTAC^C
KspI 1645 CCGCGG CCGC^GG
MaeI 2867 CTAG C^TAG
MamI 2998 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1669 TCCGGA T^CCGGA
MseI 792 TTAA T^TAA
NarI 4252 GGCGCC GG^CGCC
NotI 4277 GCGGCCGC GC^GGCCGC
PshBI 791 ATTAAT AT^TAAT
RcaI 1032 TCATGA T^CATGA
SacII 1645 CCGCGG CCGC^GG
SapI 2764 GCTCTTC GCTCTTC(1/4)
SbfI 2059 CCTGCAGG CCTGCA^GG
SfoI 4252 GGCGCC GGC^GCC
SphI 66 GCATGC GCATG^C
Sse8387I 2059 CCTGCAGG CCTGCA^GG
SspBI 2475 TGTACA T^GTACA
TfiI 3820 GAATC G^A[AT]TC
Tru9I 792 TTAA T^TAA
Tsp509I 3691 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 1082 GTCTAC GT^[AC][GT]AC
2600 GTCTAC
AviII 1135 TGCGCA TGC^GCA
1281 TGCGCA
BbrPI 1151 CACGTG CAC^GTG
1547 CACGTG
BsmBI 2446 CGTCTC CGTCTC(1/5)
535 GAGACG
BssSI 1994 CACGAG CACGAG(-5/-1)
1049 CTCGTG
BstEII 3708 GGTGACC G^GT[ACGT]ACC
4184 GGTGACC
BstPI 3708 GGTGACC G^GT[ACGT]ACC
4184 GGTGACC
BstXI 1260 CCACGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3559 CCACCCAGGTGG
DraIII 1547 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
3456 CACCAGGTG
EcoO65I 3708 GGTGACC G^GT[ACGT]ACC
4184 GGTGACC
FspI 1135 TGCGCA TGC^GCA
1281 TGCGCA
PmaCI 1151 CACGTG CAC^GTG
1547 CACGTG
PmlI 1151 CACGTG CAC^GTG
1547 CACGTG
PshAI 1659 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2734 GACCATCGTC
SacI 1076 GAGCTC GAGCT^C
2792 GAGCTC
SmlI 368 CTCAAG C^T[CT][GA]AG
1175 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
Asp700I AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BclI BcnI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssHII BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmaI StuI StyI TaqI TseI
Tsp45I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmaCI XmaI XmnI