##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Aor51HI AscI Asp700I AspEI AviII
BbeI BciVI BfrI BglII BsaBI
BsiWI BsmBI BsmI Bsp1407I BspDI
BsrGI BssHII Bst1107I BstZ17I ClaI
CpoI DraI Eam1105I Eco47III EcoT22I
FseI FspI HindIII KasI KspI
MamI MfeI MluI MunI NaeI
NarI NgoMIV NheI NotI NruI
NsiI PacI PinAI PmeI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 1578 GGTACC G^GTACC
AccI 3523 GTCTAC GT^[AC][GT]AC
AcyI 1252 GGCGTC G[GA]^CG[CT]C
AflIII 2045 ACATGT A^C[GA][CT]GT
AseI 3896 ATTAAT AT^TAAT
AsnI 3896 ATTAAT AT^TAAT
Asp718I 1578 GGTACC G^GTACC
AspI 3090 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2653 CCTAGG C^CTAGG
BbrPI 760 CACGTG CAC^GTG
BlnI 2653 CCTAGG C^CTAGG
BsaHI 1252 GGCGTC G[GA]^CG[CT]C
BsiEI 2145 CGGCCG CG[GA][CT]^CG
BspLU11I 2045 ACATGT A^CATGT
BsrFI 82 ACCGGC [GA]^CCGG[CT]
BssSI 2678 CACGAG CACGAG(-5/-1)
BstBI 162 TTCGAA TT^CGAA
BstEII 2622 GGTCACC G^GT[ACGT]ACC
BstPI 2622 GGTCACC G^GT[ACGT]ACC
Cfr10I 82 ACCGGC [GA]^CCGG[CT]
DraIII 1307 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2145 CGGCCG C^GGCCG
EclXI 2145 CGGCCG C^GGCCG
Eco52I 2145 CGGCCG C^GGCCG
EcoO65I 2622 GGTCACC G^GT[ACGT]ACC
EcoRI 358 GAATTC G^AATTC
Hin1I 1252 GGCGTC G[GA]^CG[CT]C
HpaI 4028 GTTAAC GTT^AAC
KpnI 1578 GGTACC GGTAC^C
NdeI 4054 CATATG CA^TATG
NspV 162 TTCGAA TT^CGAA
PflFI 3090 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 760 CACGTG CAC^GTG
PmlI 760 CACGTG CAC^GTG
PshBI 3896 ATTAAT AT^TAAT
PstI 3440 CTGCAG CTGCA^G
SapI 487 GAAGAGC GCTCTTC(1/4)
SfuI 162 TTCGAA TT^CGAA
SmaI 1739 CCCGGG CCC^GGG
SphI 1986 GCATGC GCATG^C
SspI 172 AATATT AAT^ATT
Tth111I 3090 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1739 CCCGGG C^CCGGG
XmaI 1739 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 1237 CGCG CG^CG
1655 CGCG
AcsI 358 GAATTC [GA]^AATT[CT]
3570 AAATTC
Alw44I 832 GTGCAC G^TGCAC
1805 GTGCAC
ApaI 2191 GGGCCC GGGCC^C
2516 GGGCCC
ApaLI 832 GTGCAC G^TGCAC
1805 GTGCAC
ApoI 358 GAATTC [GA]^AATT[CT]
3570 AAATTC
BamHI 1997 GGATCC G^GATCC
2310 GGATCC
BbsI 265 GAAGAC GAAGAC(2/6)
2590 GAAGAC
BbvCI 1078 CCTCAGC CCTCAGC(-5/-2)
3591 CCTCAGC
BcgI 3338 CGATGCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2135 GCAGCCAGATCG
BclI 2094 TGATCA T^GATCA
3369 TGATCA
BlpI 1672 GCTGAGC GC^T[ACGT]AGC
2662 GCTGAGC
Bpu1102I 1672 GCTGAGC GC^T[ACGT]AGC
2662 GCTGAGC
BpuAI 265 GAAGAC GAAGAC(2/6)
2590 GAAGAC
BsaAI 760 CACGTG [CT]AC^GT[GA]
1702 TACGTG
BspHI 2579 TCATGA T^CATGA
4000 TCATGA
BstAPI 1044 GCACGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1807 GCACAGCTTGC
BstUI 1237 CGCG CG^CG
1655 CGCG
CelII 1672 GCTGAGC GC^T[ACGT]AGC
2662 GCTGAGC
DrdI 148 GACTCCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
871 GACCCCAGTGTC
EcoRV 3201 GATATC GAT^ATC
3996 GATATC
FbaI 2094 TGATCA T^GATCA
3369 TGATCA
HaeII 1634 GGCGCT [GA]GCGC^[CT]
1822 GGCGCT
HgaI 1253 GCGTC GACGC(5/10)
1887 GCGTC
HincII 2173 GTCAAC GT[CT]^[GA]AC
4028 GTTAAC
HindII 2173 GTCAAC GT[CT]^[GA]AC
4028 GTTAAC
MvnI 1237 CGCG CG^CG
1655 CGCG
PaeR7I 1066 CTCGAG C^TCGAG
3144 CTCGAG
PflMI 252 CCAAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3414 CCATTTGGTGG
RcaI 2579 TCATGA T^CATGA
4000 TCATGA
SexAI 1001 ACCAGGT A^CC[AT]GGT
1308 ACCTGGT
Van91I 252 CCAAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3414 CCATTTGGTGG
XhoI 1066 CTCGAG C^TCGAG
3144 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccIII AciI AfaI AluI AlwI
AlwNI AocI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII